Publications
2023
2023
Activity and Crystal Structure of the Adherent-Invasive Escherichia coli Tle3/Tli3 T6SS Effector/Immunity Complex Determined Using an AlphaFold2 Predicted Model
Thi Thu Hang Le, Christine Kellenberger, Marie Boyer, Pierre Santucci, Nicolas Flaugnatti, Eric Cascales, Alain Roussel, Stéphane Canaan, Laure Journet, Christian Cambillau
International Journal of Molecular Sciences 24:1740 (2023)10.3390/ijms24021740
2022
2022
Structure Prediction and Analysis of Hepatitis E Virus Non-Structural Proteins from the Replication and Transcription Machinery by AlphaFold2
Adeline Goulet, Christian Cambillau, Alain Roussel, Isabelle Imbert
Viruses 14 (2022)10.3390/v14071537
2022
SARS-CoV-2 detection using a nanobody-functionalized voltammetric device
Quentin Pagneux, Alain Roussel, Hiba Saada, Christian Cambillau, Béatrice Amigues, Vincent Delauzun, Ilka Engelmann, Enagnon Kazali Alidjinou, Judith Ogiez, Anne Sophie Rolland, Julien Poissy, Alain Duhamel, Rabah Boukherroub, David Devos, Sabine Szunerits
Communications Medicine 2:56 (2022)10.1038/s43856-022-00113-8
2022
[Review] The role of the surface ligand on the performance of electrochemical SARS-CoV-2 antigen biosensors
Sabine Szunerits, Quentin Pagneux, Abir Swaidan, Vladyslav Mishyn, Alain Roussel, Christian Cambillau, David Devos, Ilka Engelmann, Enagnon Kazali Alidjinou, Henri Happy, Rabah Boukherroub
Analytical and Bioanalytical Chemistry 414:pp 103-113 (2022)10.1007/s00216-020-03137-y
2021
2021
A new non-classical fold of varroa odorant-binding proteins reveals a wide open internal cavity
Beatrice Amigues, Jiao Zhu, Anais Gaubert, Giovanni Renzone, Philippe Leone, Simona Arena, Isabella Maria Fischer, Harald Paulsen, Wolfgang Knoll, Andrea Scaloni, Alain Roussel, Christian Cambillau, Paolo Pelosi
Scientific Reports (2021)
2021
Preanalytical issues and cycle threshold values in SARS-CoV-2 real-time RT-PCR testing: should test results include these?
Ilka Engelmann, Enagnon Kazali Alidjinou, Judith Ogiez, Quentin Pagneux, Sana Miloudi, Ilyes Benhalima, Mahdi Ouafi, Famara Sané, Didier Hober, Alain Roussel, Christian Cambillau, David Devos, Rabah Boukherroub, Sabine Szunerits
ACS Omega 6:6528-6536 (2021)10.1021/acsomega.1c00166
2021
Anchoring the T6SS to the Cell Wall: Crystal Structure of the Peptidoglycan Binding Domain of the TagL Accessory Protein
Van Son Nguyen, Silvia Spinelli, Éric Cascales, Alain Roussel, Christian Cambillau, Philippe Leone
PLoS ONE (2021)
2021
Anchoring the T6SS to the cell wall: Crystal structure of the peptidoglycan binding domain of the TagL accessory protein
van Son Nguyen, Silvia Spinelli, E. Cascales, Alain Roussel, Christian Cambillau, Philippe Leone
PLoS ONE 16:e0254232 (2021)10.1371/journal.pone.0254232
2021
Crystal structures of two camelid nanobodies raised against GldL, a component of the Type IX Secretion System from Flavobacterium johnsoniae
Thi Trang Nhung, Anaïs Gaubert, Pauline Melani, Christian Cambillau, Alain Roussel, Philippe Leone
Acta crystallographica Section F : Structural biology communications [2014-...] (2021)
2021
Macromolecular interactions in vitro, comparing classical and novel approaches
Christophe Velours, Magali Aumont-Nicaise, Stephan Uebel, Patrick England, Adrian Velazquez-Campoy, David Stroebel, Guillaume Bec, Pierre Soule, Christophe Quétard, Christine Ebel, Alain Roussel, Jean-Baptiste Charbonnier, Paloma Fernández Varela
European Biophysics Journal 50:313-330 (2021)10.1007/s00249-021-01517-5
2020
2020
Extracellular vesicles as a platform to study cell-surface membrane proteins
Vincent Delauzun, Beatrice Amigues, Anais Gaubert, Philippe Leone, Magali Grange, Laurent Gauthier, Alain Roussel
Methods 180:35-44 (2020)10.1016/j.ymeth.2020.03.004
2020
Crystal structure of Type IX secretion system PorE C-terminal domain from Porphyromonas gingivalis in complex with a peptidoglycan fragment
Nhung Thi Trang Trinh, Hieu Quang Tran, Quyen van Dong, Christian Cambillau, Alain Roussel, Philippe Leone
Scientific Reports 10 (2020)10.1038/s41598-020-64115-z
2019
2019
A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography
Raphaël de Wijn, Oliver Hennig, Jennifer Roche, Sylvain Engilberge, Kevin Rollet, Pablo Fernandez-Millan, Karl Brillet, Heike Betat, Mario Mörl, Alain Roussel, Eric Girard, Christoph Mueller-Dieckmann, Gavin Fox, Vincent Olieric, José Gavira, Bernard Lorber, Claude Sauter
International Union of Crystallography journal 6:454-464 (2019)10.1107/S2052252519003622
2019
Cas9 Allosteric Inhibition by the Anti-CRISPR Protein AcrIIA6
Olivier Fuchsbauer, Paolo Swuec, Claire Zimberger, Beatrice Amigues, Sébastien Levesque, Daniel Agudelo, Alexis Duringer, Antonio Chaves-Sanjuan, Silvia Spinelli, Geneviève Rousseau, Minja Velimirovic, Martino Bolognesi, Alain Roussel, Christian C. Cambillau, Sylvain Moineau, Yannick Doyon, Adeline Goulet
Molecular Cell 76:922-937.e7 (2019)10.1016/j.molcel.2019.09.012
2019
Multifunctional Natural Killer Cell Engagers Targeting NKp46 Trigger Protective Tumor Immunity
Laurent Gauthier, Ariane Morel, Nadia Anceriz, Benjamin Rossi, Audrey Blanchard-Alvarez, Gwendoline Grondin, Sylvia Trichard, Cedric Cesari, Melody Sapet, Frederic Bosco, Helene Rispaud-Blanc, Franceline Guillot, Stephanie Cornen, Alain Roussel, Beatrice Amigues, Guillaume Habif, Flavien Carague, Sandrine Arrufat, Romain Remark, Francois Romagne, Yannis Morel, Emilie Narni-Mancinelli, Eric Vivier
Cell 177:1701+ (2019)10.1016/j.cell.2019.04.041
2019
Blocking Antibodies Targeting the CD39/CD73 Immunosuppressive Pathway Unleash Immune Responses in Combination Cancer Therapies
Ivan Perrot, Henri-Alexandre Michaud, Marc Giraudon-Paoli, Severine Augier, Aurelie Docquier, Laurent Gros, Rachel Courtois, Cecile Dejou, Diana Jecko, Ondine Becquart, Helene Rispaud-Blanc, Laurent Gauthier, Benjamin Rossi, Stephanie Chanteux, Nicolas Gourdin, Beatrice Amigues, Alain Roussel, Armand Bensussan, Jean-Francois Eliaou, Jeremy Bastid, Francois Romagne, Yannis Morel, Emilie Narni-Mancinelli, Eric Vivier, Carine Paturel, Nathalie Bonnefoy
Cell Reports 27:2411-2425 (2019)10.1016/j.celrep.2019.04.091
2019
Novel ACTN1 variants in cases of thrombocytopenia
Anne Vincenot, Paul Saultier, Shinji Kunishima, Marjorie Poggi, Marie-Françoise Hurtaud-Roux, Alain Roussel, Christine Biron-Andreani, Jean-François Claisse, Remy Dulery, Eric Durot, Véronique Fiore, Rémi Favier, Pierre P. Fenaux, Felipe Guerrero, Véronique Le Cam-Duchez, Elisabeth Mazoyer, Stephanie Müller, Benedicte Neven, Catherine Pouymayou, Bruno Royer, Pierre Sie, Sandrine Thouvenin, Catherine Trichet, Nathalie Trillot, Karima Yakouben, Schlegel Nicole, Marie-Christine Alessi
Human Mutation 40:2258-2269 (2019)10.1002/humu.23840
2018
2018
Type IX secretion system PorM and gliding machinery GldM form extended arches spanning the periplasmic space
Philippe Leone, Jennifer Roche, Maxence S Vincent, Quang Hieu Tran, Aline Desmyter, E. Cascales, Christine Kellenberger, Christian Cambillau, Alain Roussel
Nature Communications 9 (2018)10.1038/s41467-017-02784-7
2018
Towards a complete structural deciphering of Type VI secretion system
van Son Nguyen, Badreddine Douzi, Eric Durand, Alain Roussel, E. Cascales, Christian Cambillau
Current Opinion in Structural Biology 49:77-84 (2018)10.1016/j.sbi.2018.01.007
2017
2017
Unraveling the Self-Assembly of the Pseudomonas aeruginosa XcpQ Secretin Periplasmic Domain Provides New Molecular Insights into Type II Secretion System Secreton Architecture and Dynamics
Badreddine Douzi, Nhung Trinh, Sandra Michel-Souzy, Aline Desmyter, Genevieve Ball, Pascale Barbier, Artemis Kosta, Eric Durand, Katrina Forest, Christian Cambillau, Alain Roussel, Romé Voulhoux
mBio 8 (2017)10.1128/mBio.01185-17
2017
Camelid nanobodies used as crystallization chaperones for different constructs of PorM, a component of the type IX secretion system from Porphyromonas gingivalis
Yoan Duhoo, Jennifer Roche, Thi Trang Nhung Trinh, Aline Desmyter, Anaïs Gaubert, Christine Kellenberger, Christian Cambillau, Alain Roussel, Philippe Leone
Acta crystallographica Section F : Structural biology communications [2014-...] 73:286-293 (2017)10.1107/S2053230X17005969
2017
Probing Conformational Changes and Interfacial Recognition Site of Lipases With Surfactants and Inhibitors
Eduardo Mateos Diaz, S, Amara, A Roussel, Sonia Longhi, Christian Cambillau, Frédéric Carrière
Methods in Enzymology 279-307 (2017)
2017
Type VI secretion TssK baseplate protein exhibits structural similarity with phage receptor-binding proteins and evolved to bind the membrane complex
van Son Nguyen, Laureen Logger, Silvia Spinelli, Pierre Legrand, Thi Thanh Huyen Pham, Thi Trang Nhung Trinh, Yassine Cherrak, Abdelrahim Zoued, Aline Desmyter, Eric Durand, Alain Roussel, Christine Kellenberger, E. Cascales, Christian Cambillau
Nature Microbiology 2:17103 (2017)10.1038/nmicrobiol.2017.103
2017
Characterization of the Porphyromonas gingivalis Type IX Secretion Trans-envelope PorKLMNP Core Complex
Maxence S Vincent, Mickaël J Canestrari, Philippe Leone, Julien Stathopulos, Bérengère Ize, Abdelrahim Zoued, Christian Cambillau, Christine Kellenberger, Alain Roussel, E. Cascales
Journal of Biological Chemistry 292:3252-3261 (2017)10.1074/jbc.M116.765081
2017
TssA: The cap protein of the Type VI secretion system tail
Abdelrahim Zoued, Eric Durand, Yoann G Santin, Laure Journet, Alain Roussel, Christian Cambillau, E. Cascales
BioEssays 39 (2017)10.1002/bies.201600262
2016
2016
A phospholipase A 1 antibacterial Type VI secretion effector interacts directly with the C-terminal domain of the VgrG spike protein for delivery
Nicolas Flaugnatti, Thi Thu Hang Le, Stéphane Canaan, Marie-Stéphanie Aschtgen, Vân Nguyên, Stephanie Blangy, Christine Kellenberger, Alain Roussel, Christian Cambillau, E. Cascales, Laure Journet
Molecular Microbiology 99:1099-1118 (2016)10.1111/mmi.13292
2016
Structure of the host-recognition device of Staphylococcus aureus phage phi 11
Cengiz Koç, Guoqing Xia, Petra Kuehner, Silvia Spinelli, Alain Roussel, Christian Cambillau, Thilo Stehle
Scientific Reports 6 (2016)10.1038/srep27581
2016
Cytokine Diedel and a viral homologue suppress the IMD pathway in Drosophila
Olivier Lamiable, Christine Kellenberger, Cordula Kemp, Laurent Troxler, Nadege Pelte, Michael Boutros, Joao Trindade Marques, Laurent Daeffler, Jules A. Hoffmann, Alain Roussel, Jean-Luc Imler
Proceedings of the National Academy of Sciences of the United States of America 113:698-703 (2016)10.1073/pnas.1516122113
2015
2015
Tissue-Specific Regulation of Drosophila NF-x03BA;B Pathway Activation by Peptidoglycan Recognition Protein SC
D. Costechareyre, F. Capo, A. Fabre, D. Chaduli, C. Kellenberger, A. Roussel, B. Charroux, J. Royet
Journal of Innate Immunity 8:67-80 (2015)10.1159/000437368
2015
Camelid nanobodies: killing two birds with one stone
Aline Desmyter, Silvia Spinelli, Alain Roussel, Christian Cambillau
Current Opinion in Structural Biology 32:1-8 (2015)10.1016/j.sbi.2015.01.001
2015
Biogenesis and structure of a Type VI secretion membrane core complex
Eric Durand, van Son Nguyen, Abdelrahim Zoued, Laureen Logger, Gerard Pehau-Arnaudet, Marie-Stéphanie Aschtgen, Silvia Spinelli, Aline Desmyter, Benjamin Bardiaux, Annick Dujeancourt, Alain Roussel, Christian Cambillau, E. Cascales, Rémi Fronzes
Nature 523:555-560 (2015)10.1038/nature14667
2015
X-ray and Cryo-electron Microscopy Structures of Monalysin Pore-forming Toxin Reveal Multimerization of the Pro-form
Philippe Leone, Cecilia Bebeacua, Onya Opota, Christine Kellenberger, Bruno Klaholz, Igor Orlov, Christian Cambillau, Bruno Lemaitre, Alain Roussel
Journal of Biological Chemistry 290:13191-13201 (2015)10.1074/jbc.M115.646109
2015
Inhibition of Type VI Secretion by an Anti-TssM Llama Nanobody
van Son Nguyen, Laureen Logger, Silvia Spinelli, Aline Desmyter, Thi Thu Hang Le, Christine Kellenberger, Badreddine Douzi, Eric Durand, Alain Roussel, E. Cascales, Christian Cambillau
PLoS ONE 10:1-14 (2015)
2015
Production, crystallization and X-ray diffraction analysis of a complex between a fragment of the TssM T6SS protein and a camelid nanobody
van Son Nguyen, Silvia Spinelli, Aline Desmyter, Thi Thu Hang Le, Christine Kellenberger, E. Cascales, Christian Cambillau, Alain Roussel
Acta crystallographica Section F : Structural biology communications [2014-...] 71:266-271 (2015)10.1107/S2053230X15000709
2015
Development and evaluation of a sublingual tablet based on recombinant Bet v 1 in birch pollen-allergic patients
E. Nony, J. Bouley, M. Le Mignon, P. Lemoine, K. Jain, S. Horiot, L. Mascarell, M. Pallardy, R. Vincentelli, Philippe Leone, A. Roussel, T. Batard, K. Abiteboul, B. Robin, O. de Beaumont, M. Arvidsson, S. Rak, P. Moingeon
Allergy 70:795-804 (2015)10.1111/all.12622
2015
Crystallization and preliminary X-ray analysis of the C-terminal fragment of PorM, a subunit of the Porphyromonas gingivalis type IX secretion system
Julien Stathopulos, Christian Cambillau, E. Cascales, Alain Roussel, Philippe Leone
Acta crystallographica Section F : Structural biology communications [2014-...] 71:71-74 (2015)10.1107/S2053230X1402559X
2014
2014
Bile-mediated activation of the acrAB and tolC multidrug efflux genes occurs mainly through transcriptional derepression of ramA in [i]Salmonella enterica[/i] serovar Typhimurium
Sylvie Baucheron-Monnier, Kunihiko Nishino, Isabelle Monchaux, Sylvie Canepa, Marie-Christine Maurel, Franck Coste, Alain Roussel, Axel Cloeckaert, Etienne Giraud
Journal of Antimicrobial Chemotherapy 69:2400-2406 (2014)10.1093/jac/dku140
2014
Crystal Structure and Self-Interaction of the Type VI Secretion Tail-Tube Protein from Enteroaggregative Escherichia coli
Badreddine Douzi, Silvia Spinelli, Stéphanie Blangy, Alain Roussel, Eric Durand, Yannick Brunet, E. Cascales, Christian Cambillau
PLoS ONE 9:e86918 (2014)10.1371/journal.pone.0086918
2014
A Cutinase from Trichoderma reesei with a Lid-Covered Active Site and Kinetic Properties of True Lipases
Alain Roussel, Sawsan Amara, Antti Nyyssölä, Eduardo Mateos-Diaz, Stéphanie Blangy, Hanna Kontkanen, Ann Westerholm-Parvinen, Frédéric Carrière, Christian Cambillau
Journal of Molecular Biology 426:3757-3772 (2014)10.1016/j.jmb.2014.09.003
2013
2013
Molecular engineering of fungal GH5 and GH26 beta-(1,4)-mannanases toward improvement of enzyme activity
Marie M. Couturier, Julia Feliu, Sophie Bozonnet, Alain Roussel, Jean-Guy J.-G. Berrin
PLoS ONE 8 (2013)10.1371/journal.pone.0079800
2013
Functional Analysis of PGRP-LA in Drosophila Immunity
Mathilde Gendrin, Anna Zaidman-Rémy, Nichole A Broderick, Juan Paredes, Mickaël Poidevin, Alain Roussel, Bruno Lemaitre
PLoS ONE 8:e69742 (2013)10.1371/journal.pone.0069742
2013
Crystallization and preliminary X-ray analysis of monalysin, a novel β-pore-forming toxin from the entomopathogen Pseudomonas entomophila
Philippe Leone, Maryline Blemont, Renaud Vincentelli, Christine Kellenberger, Onya Opota, Bruno Lemaitre, Alain Roussel
Acta Crystallographica Section F: Structural Biology and Crystallization Communications 69:930-933 (2013)10.1107/S174430911301885X
2012
2012
Binding of the RamR repressor to wild-type and mutated promoters of the RamA gene involved in efflux-mediated multidrug resistance in Salmonella enterica serovar Typhimurium.
Sylvie Baucheron, Franck Coste, Sylvie Canepa, Marie-Christine Maurel, Etienne Giraud, Françoise Culard, Bertrand Castaing, Alain Roussel, Axel Cloeckaert
Antimicrobial Agents and Chemotherapy 56:942-8 (2012)10.1128/AAC.05444-11
2012
Crystal structure of Diedel, a marker of the immune response of Drosophila melanogaster.
Franck Coste, Cordula Kemp, Vanessa Bobezeau, Charles Hetru, Christine Kellenberger, Jean-Luc Imler, Alain Roussel
PLoS ONE 7:e33416 (2012)10.1371/journal.pone.0033416
2012
Crystal structure of greglin, a novel non-classical Kazal inhibitor, in complex with subtilisin.
Chrystelle Derache, Christophe Epinette, Alain Roussel, Guillaume Gabant, Martine Cadene, Brice Korkmaz, Francis Gauthier, Christine Kellenberger
FEBS Journal 279:4466-78 (2012)10.1111/febs.12033
2011
2011
The Drosophila peptidoglycan-recognition protein LF interacts with peptidoglycan-recognition protein LC to downregulate the Imd pathway
Nada Basbous, Franck Coste, Philippe Leone, Renaud Vincentelli, Julien Royet, Christine Kellenberger, Alain Roussel
EMBO Reports 12:327-333 (2011)10.1038/embor.2011.19
2011
Structure-Function Analysis of Grass Clip Serine Protease Involved in Drosophila Toll Pathway Activation
Christine Kellenberger, Philippe Leone, Laurent Coquet, Thierry Jouenne, Jean-Marc Reichhart, Alain Roussel
Journal of Biological Chemistry 286:12300-12307 (2011)10.1074/jbc.M110.182741
2009
2009
Expression, purification, crystallization and preliminary X-ray analysis of the N-terminal domain of GNBP3 from Drosophila melanogaster
Yumiko Mishima, Franck Coste, Vanessa Bobezeau, Nadège Hervouet, Christine Kellenberger, Alain Roussel
Acta crystallographica Section F : Structural biology communications [2014-...] 65:870-873 (2009)10.1107/S1744309109014997
2009
The N-terminal Domain of Drosophila Gram-negative Binding Protein 3 (GNBP3) Defines a Novel Family of Fungal Pattern Recognition Receptors
Yumiko Mishima, Jessica Quintin, Vishukumar Aimanianda, Christine Kellenberger, Franck Coste, Cécile Clavaud, A. Hoffmann Jules, Jean-Paul Latgé, Dominique Ferrandon, Alain Roussel
Journal of Biological Chemistry 284:28687-28697 (2009)10.1074/jbc.M109.034587
2008
2008
Crystal structure of Drosophila PGRP-SD suggests binding to DAP-type but not lysine-type peptidoglycan.
P. Leone, V. Bischoff, C. Kellenberger, C. Hetru, J. Royet, A. Roussel
Molecular Immunology 45:2521-2530 (2008)
2008
Crystal structure of Drosophila PGRP-SD suggests binding to DAP-type but not lysine-type peptidoglycan
Philippe Leone, Vincent Bischoff, Christine Kellenberger, Charles Hetru, Julien Royetd, Alain Roussel
Molecular Immunology 45:2521-2530 (2008)10.1016/j.molimm.2008.01.015
2008
Structure of Locusta migratoria protease inhibitor 3 (LMPI-3) in complex with Fusarium oxysporum trypsin
Philippe Leone, Alain Roussel, Christine Kellenberger
Acta crystallographica Section D : Structural biology [1993-...] 64:1165-1171 (2008)10.1107/S0907444908030400
2007
2007
How much can a T-cell antigen receptor adapt to structurally distinct antigenic peptides?
Catherine Mazza, Nathalie Auphan-Anezin, Claude Gregoire, Annick Guimezanes, Christine Kellenberger, Alain Roussel, Alice Kearney, P Anton van Der Merwe, Anne-Marie Schmitt-Verhulst, Bernard Malissen
EMBO Journal 26:1972-83 (2007)10.1038/sj.emboj.7601605
2003
2003
Selective Inhibition of Trypsins by Insect Peptides: Role of P6−P10 Loop
Philippe Leone, C. Kellenberger, G. Ferrat, H. Darbon, A. Roussel
Biochemistry 42:13605-13612 (2003)10.1021/bi035318t
2002
2002
A medium-throughput crystallization approach
Gerlind Sulzenbacher, Arnaud Gruez, Véronique Roig-Zamboni, Silvia Spinelli, Christel Valencia, Fabienne Pagot, Renaud Vincentelli, Christophe Bignon, Aurelia Salomoni, Sacha Grisel, Damien Maurin, Céline Huyghe, Kent Johansson, Alice Grassick, Alain Roussel, Yves Bourne, Sophie Perrier, Linda Miallau, Phillippe Cantau, Eric Blanc, Michel Genevois, Alain Grossi, André Zenatti, Valérie Campanacci, Christian Cambillau
Acta crystallographica Section D : Structural biology [1993-...] 58:2109-2115 (2002)10.1107/s0907444902013938
2001
2001
The fusion glycoprotein shell from Semliki Forest Virus: an icosahedral assembly primed for fusogenic activation at endosomal pH
J. Lescar, A. Roussel, M.W. Wien, J. Navaza, S.D. Fuller, G. Wengler, F.A. Rey
Cell 137-148 (2001)