Research group: Host-Pathogen InteractionGroup leader

Phone: +33 (0)4 91 16 41 24

Room: BM303

Publications

2022

2022
SARS-CoV-2 detection using a nanobody-functionalized voltammetric device

Quentin Pagneux, Alain Roussel, Hiba Saada, Christian Cambillau, Béatrice Amigues, Vincent Delauzun, Ilka Engelmann, Enagnon Kazali Alidjinou, Judith Ogiez, Anne Sophie Rolland, Emmanuel Faure, Julien Poissy, Alain Duhamel, Rabah Boukherroub, David Devos, Sabine Szunerits

Communications Medicine 2:56 (2022)10.1038/s43856-022-00113-8

2022
[Review] The role of the surface ligand on the performance of electrochemical SARS-CoV-2 antigen biosensors

Sabine Szunerits, Quentin Pagneux, Abir Swaidan, Vladyslav Mishyn, Alain Roussel, Christian Cambillau, David Devos, Ilka Engelmann, Enagnon Kazali Alidjinou, Henri Happy, Rabah Boukherroub

Analytical and Bioanalytical Chemistry 414:pp 103-113 (2022)10.1007/s00216-020-03137-y

2021

2021
A new non-classical fold of varroa odorant-binding proteins reveals a wide open internal cavity

Beatrice Amigues, Jiao Zhu, Anais Gaubert, Giovanni Renzone, Philippe Leone, Simona Arena, Isabella Maria Fischer, Harald Paulsen, Wolfgang Knoll, Andrea Scaloni, Alain Roussel, Christian Cambillau, Paolo Pelosi

Scientific Reports (2021)

2021
Preanalytical issues and cycle threshold values in SARS-CoV-2 real-time RT-PCR testing: should test results include these?

Ilka Engelmann, Enagnon Kazali Alidjinou, Judith Ogiez, Quentin Pagneux, Sana Miloudi, Ilyes Benhalima, Mahdi Ouafi, Famara Sané, Didier Hober, Alain Roussel, Christian Cambillau, David Devos, Rabah Boukherroub, Sabine Szunerits

ACS Omega 6:6528-6536 (2021)10.1021/acsomega.1c00166

2021
Anchoring the T6SS to the Cell Wall: Crystal Structure of the Peptidoglycan Binding Domain of the TagL Accessory Protein

Van Son Nguyen, Silvia Spinelli, Éric Cascales, Alain Roussel, Christian Cambillau, Philippe Leone

PLoS ONE (2021)

2021
Anchoring the T6SS to the cell wall: Crystal structure of the peptidoglycan binding domain of the TagL accessory protein

van Son Nguyen, Silvia Spinelli, E. Cascales, Alain Roussel, Christian Cambillau, Philippe Leone

PLoS ONE 16:e0254232 (2021)10.1371/journal.pone.0254232

2021
Crystal structures of two camelid nanobodies raised against GldL, a component of the Type IX Secretion System from Flavobacterium johnsoniae

Thi Trang Nhung, Anaïs Gaubert, Pauline Melani, Christian Cambillau, Alain Roussel, Philippe Leone

Acta crystallographica Section F : Structural biology communications [2014-...] (2021)

2021
Macromolecular interactions in vitro, comparing classical and novel approaches

Christophe Velours, Magali Aumont-Nicaise, Stephan Uebel, Patrick England, Adrian Velazquez-Campoy, David Stroebel, Guillaume Bec, Pierre Soule, Christophe Quétard, Christine Ebel, Alain Roussel, Jean-Baptiste Charbonnier, Paloma Fernández Varela

European Biophysics Journal 50:313-330 (2021)10.1007/s00249-021-01517-5

2020

2020
Extracellular vesicles as a platform to study cell-surface membrane proteins

Vincent Delauzun, Beatrice Amigues, Anais Gaubert, Philippe Leone, Magali Grange, Laurent Gauthier, Alain Roussel

Methods 180:35-44 (2020)10.1016/j.ymeth.2020.03.004

2020
Crystal structure of Type IX secretion system PorE C-terminal domain from Porphyromonas gingivalis in complex with a peptidoglycan fragment

Nhung Thi Trang Trinh, Hieu Quang Tran, Quyen van Dong, Christian Cambillau, Alain Roussel, Philippe Leone

Scientific Reports 10 (2020)10.1038/s41598-020-64115-z

2019

2019
A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography

Raphaël de Wijn, Oliver Hennig, Jennifer Roche, Sylvain Engilberge, Kevin Rollet, Pablo Fernandez-Millan, Karl Brillet, Heike Betat, Mario Mörl, Alain Roussel, Eric Girard, Christoph Mueller-Dieckmann, Gavin Fox, Vincent Olieric, José Gavira, Bernard Lorber, Claude Sauter

International Union of Crystallography journal 6:454-464 (2019)10.1107/S2052252519003622

2019
Cas9 Allosteric Inhibition by the Anti-CRISPR Protein AcrIIA6

Olivier Fuchsbauer, Paolo Swuec, Claire Zimberger, Beatrice Amigues, Sébastien Levesque, Daniel Agudelo, Alexis Duringer, Antonio Chaves-Sanjuan, Silvia Spinelli, Geneviève Rousseau, Minja Velimirovic, Martino Bolognesi, Alain Roussel, Christian Cambillau, Sylvain Moineau, Yannick Doyon, Adeline Goulet

Molecular Cell 76:922-937.e7 (2019)10.1016/j.molcel.2019.09.012

2019
Blocking Antibodies Targeting the CD39/CD73 Immunosuppressive Pathway Unleash Immune Responses in Combination Cancer Therapies

Ivan Perrot, Henri-Alexandre Michaud, Marc Giraudon-Paoli, Severine Augier, Aurelie Docquier, Laurent Gros, Rachel Courtois, Cecile Dejou, Diana Jecko, Ondine Becquart, Helene Rispaud-Blanc, Laurent Gauthier, Benjamin Rossi, Stephanie Chanteux, Nicolas Gourdin, Beatrice Amigues, Alain Roussel, Armand Bensussan, Jean-Francois Eliaou, Jeremy Bastid, Francois Romagne, Yannis Morel, Emilie Narni-Mancinelli, Eric Vivier, Carine Paturel, Nathalie Bonnefoy

Cell Reports 27:2411-2425 (2019)10.1016/j.celrep.2019.04.091

2019
Novel ACTN1 variants in cases of thrombocytopenia

Anne Vincenot, Paul Saultier, Shinji Kunishima, Marjorie Poggi, Marie-Françoise Hurtaud-Roux, Alain Roussel, Christine Biron-Andreani, Jean-François Claisse, Remy Dulery, Eric Durot, Véronique Fiore, Rémi Favier, Pierre P. Fenaux, Felipe Guerrero, Véronique Le Cam-Duchez, Elisabeth Mazoyer, Stephanie Müller, Benedicte Neven, Catherine Pouymayou, Bruno Royer, Pierre Sie, Sandrine Thouvenin, Catherine Trichet, Nathalie Trillot, Karima Yakouben, Schlegel Nicole, Marie-Christine Alessi

Human Mutation 40:2258-2269 (2019)10.1002/humu.23840

2018

2018
Type IX secretion system PorM and gliding machinery GldM form extended arches spanning the periplasmic space

Philippe Leone, Jennifer Roche, Maxence S Vincent, Quang Hieu Tran, Aline Desmyter, E. Cascales, Christine Kellenberger, Christian Cambillau, Alain Roussel

Nature Communications 9 (2018)10.1038/s41467-017-02784-7

2018
Towards a complete structural deciphering of Type VI secretion system

van Son Nguyen, Badreddine Douzi, Eric Durand, Alain Roussel, E. Cascales, Christian Cambillau

Current Opinion in Structural Biology 49:77-84 (2018)10.1016/j.sbi.2018.01.007

2017

2017
Unraveling the Self-Assembly of the Pseudomonas aeruginosa XcpQ Secretin Periplasmic Domain Provides New Molecular Insights into Type II Secretion System Secreton Architecture and Dynamics

Badreddine Douzi, Nhung Trinh, Sandra Michel-Souzy, Aline Desmyter, Genevieve Ball, Pascale Barbier, Artemis Kosta, Eric Durand, Katrina Forest, Christian Cambillau, Alain Roussel, Romé Voulhoux

mBio 8 (2017)10.1128/mBio.01185-17

2017
Camelid nanobodies used as crystallization chaperones for different constructs of PorM, a component of the type IX secretion system from Porphyromonas gingivalis

Yoan Duhoo, Jennifer Roche, Thi Trang Nhung Trinh, Aline Desmyter, Anaïs Gaubert, Christine Kellenberger, Christian Cambillau, Alain Roussel, Philippe Leone

Acta crystallographica Section F : Structural biology communications [2014-...] 73:286-293 (2017)10.1107/S2053230X17005969

2017
Probing Conformational Changes and Interfacial Recognition Site of Lipases With Surfactants and Inhibitors

Eduardo Mateos Diaz, S, Amara, A Roussel, Sonia Longhi, Christian Cambillau, Frédéric Carrière

Methods in Enzymology 279-307 (2017)

2017
Type VI secretion TssK baseplate protein exhibits structural similarity with phage receptor-binding proteins and evolved to bind the membrane complex

van Son Nguyen, Laureen Logger, Silvia Spinelli, Pierre Legrand, Thi Thanh Huyen Pham, Thi Trang Nhung Trinh, Yassine Cherrak, Abdelrahim Zoued, Aline Desmyter, Eric Durand, Alain Roussel, Christine Kellenberger, E. Cascales, Christian Cambillau

Nature Microbiology 2:17103 (2017)10.1038/nmicrobiol.2017.103

2017
Characterization of the Porphyromonas gingivalis Type IX Secretion Trans-envelope PorKLMNP Core Complex

Maxence S Vincent, Mickaël J Canestrari, Philippe Leone, Julien Stathopulos, Bérengère Ize, Abdelrahim Zoued, Christian Cambillau, Christine Kellenberger, Alain Roussel, E. Cascales

Journal of Biological Chemistry 292:3252-3261 (2017)10.1074/jbc.M116.765081

2017
TssA: The cap protein of the Type VI secretion system tail

Abdelrahim Zoued, Eric Durand, Yoann G Santin, Laure Journet, Alain Roussel, Christian Cambillau, E. Cascales

BioEssays 39 (2017)10.1002/bies.201600262

2016

2016
A phospholipase A 1 antibacterial Type VI secretion effector interacts directly with the C-terminal domain of the VgrG spike protein for delivery

Nicolas Flaugnatti, Thi Thu Hang Le, Stéphane Canaan, Marie-Stéphanie Aschtgen, Vân Nguyên, Stephanie Blangy, Christine Kellenberger, Alain Roussel, Christian Cambillau, E. Cascales, Laure Journet

Molecular Microbiology 99:1099-1118 (2016)10.1111/mmi.13292

2016
Structure of the host-recognition device of Staphylococcus aureus phage phi 11

Cengiz Koç, Guoqing Xia, Petra Kuehner, Silvia Spinelli, Alain Roussel, Christian Cambillau, Thilo Stehle

Scientific Reports 6 (2016)10.1038/srep27581

2016
Cytokine Diedel and a viral homologue suppress the IMD pathway in Drosophila

Olivier Lamiable, Christine Kellenberger, Cordula Kemp, Laurent Troxler, Nadege Pelte, Michael Boutros, Joao Trindade Marques, Laurent Daeffler, Jules A. Hoffmann, Alain Roussel, Jean-Luc Imler

Proceedings of the National Academy of Sciences of the United States of America 113:698-703 (2016)10.1073/pnas.1516122113

2015

2015
Tissue-Specific Regulation of Drosophila NF-x03BA;B Pathway Activation by Peptidoglycan Recognition Protein SC

D. Costechareyre, F. Capo, A. Fabre, D. Chaduli, C. Kellenberger, A. Roussel, B. Charroux, J. Royet

Journal of Innate Immunity 8:67-80 (2015)10.1159/000437368

2015
Camelid nanobodies: killing two birds with one stone

Aline Desmyter, Silvia Spinelli, Alain Roussel, Christian Cambillau

Current Opinion in Structural Biology 32:1-8 (2015)10.1016/j.sbi.2015.01.001

2015
Biogenesis and structure of a Type VI secretion membrane core complex

Eric Durand, van Son Nguyen, Abdelrahim Zoued, Laureen Logger, Gerard Pehau-Arnaudet, Marie-Stéphanie Aschtgen, Silvia Spinelli, Aline Desmyter, Benjamin Bardiaux, Annick Dujeancourt, Alain Roussel, Christian Cambillau, E. Cascales, Rémi Fronzes

Nature 523:555-560 (2015)10.1038/nature14667

2015
X-ray and Cryo-electron Microscopy Structures of Monalysin Pore-forming Toxin Reveal Multimerization of the Pro-form

Philippe Leone, Cecilia Bebeacua, Onya Opota, Christine Kellenberger, Bruno Klaholz, Igor Orlov, Christian Cambillau, Bruno Lemaitre, Alain Roussel

Journal of Biological Chemistry 290:13191-13201 (2015)10.1074/jbc.M115.646109

2015
Inhibition of Type VI Secretion by an Anti-TssM Llama Nanobody

van Son Nguyen, Laureen Logger, Silvia Spinelli, Aline Desmyter, Thi Thu Hang Le, Christine Kellenberger, Badreddine Douzi, Eric Durand, Alain Roussel, E. Cascales, Christian Cambillau

PLoS ONE 10:1-14 (2015)

2015
Production, crystallization and X-ray diffraction analysis of a complex between a fragment of the TssM T6SS protein and a camelid nanobody

van Son Nguyen, Silvia Spinelli, Aline Desmyter, Thi Thu Hang Le, Christine Kellenberger, E. Cascales, Christian Cambillau, Alain Roussel

Acta crystallographica Section F : Structural biology communications [2014-...] 71:266-271 (2015)10.1107/S2053230X15000709

2015
Development and evaluation of a sublingual tablet based on recombinant Bet v 1 in birch pollen-allergic patients

E. Nony, J. Bouley, M. Le Mignon, P. Lemoine, K. Jain, S. Horiot, L. Mascarell, M. Pallardy, R. Vincentelli, Philippe Leone, A. Roussel, T. Batard, K. Abiteboul, B. Robin, O. de Beaumont, M. Arvidsson, S. Rak, P. Moingeon

Allergy 70:795-804 (2015)10.1111/all.12622

2015
Crystallization and preliminary X-ray analysis of the C-terminal fragment of PorM, a subunit of the Porphyromonas gingivalis type IX secretion system

Julien Stathopulos, Christian Cambillau, E. Cascales, Alain Roussel, Philippe Leone

Acta crystallographica Section F : Structural biology communications [2014-...] 71:71-74 (2015)10.1107/S2053230X1402559X

2014

2014
Bile-mediated activation of the acrAB and tolC multidrug efflux genes occurs mainly through transcriptional derepression of ramA in [i]Salmonella enterica[/i] serovar Typhimurium

Sylvie Baucheron-Monnier, Kunihiko Nishino, Isabelle Monchaux, Sylvie Canepa, Marie-Christine Maurel, Franck Coste, Alain Roussel, Axel Cloeckaert, Etienne Giraud

Journal of Antimicrobial Chemotherapy 69:2400-2406 (2014)10.1093/jac/dku140

2014
Crystal Structure and Self-Interaction of the Type VI Secretion Tail-Tube Protein from Enteroaggregative Escherichia coli

Badreddine Douzi, Silvia Spinelli, Stéphanie Blangy, Alain Roussel, Eric Durand, Yannick Brunet, E. Cascales, Christian Cambillau

PLoS ONE 9:e86918 (2014)10.1371/journal.pone.0086918

2014
A Cutinase from Trichoderma reesei with a Lid-Covered Active Site and Kinetic Properties of True Lipases

Alain Roussel, Sawsan Amara, Antti Nyyssölä, Eduardo Mateos-Diaz, Stéphanie Blangy, Hanna Kontkanen, Ann Westerholm-Parvinen, Frédéric Carrière, Christian Cambillau

Journal of Molecular Biology 426:3757-3772 (2014)10.1016/j.jmb.2014.09.003

2013

2013
Molecular engineering of fungal GH5 and GH26 beta-(1,4)-mannanases toward improvement of enzyme activity

Marie M. Couturier, Julia Feliu, Sophie Bozonnet, Alain Roussel, Jean-Guy J.-G. Berrin

PLoS ONE 8 (2013)10.1371/journal.pone.0079800

2013
Functional Analysis of PGRP-LA in Drosophila Immunity

Mathilde Gendrin, Anna Zaidman-Rémy, Nichole A Broderick, Juan Paredes, Mickaël Poidevin, Alain Roussel, Bruno Lemaitre

PLoS ONE 8:e69742 (2013)10.1371/journal.pone.0069742

2013
Crystallization and preliminary X-ray analysis of monalysin, a novel β-pore-forming toxin from the entomopathogen Pseudomonas entomophila

Philippe Leone, Maryline Blemont, Renaud Vincentelli, Christine Kellenberger, Onya Opota, Bruno Lemaitre, Alain Roussel

Acta Crystallographica Section F: Structural Biology and Crystallization Communications 69:930-933 (2013)10.1107/S174430911301885X

2012

2012
Binding of the RamR repressor to wild-type and mutated promoters of the RamA gene involved in efflux-mediated multidrug resistance in Salmonella enterica serovar Typhimurium.

Sylvie Baucheron, Franck Coste, Sylvie Canepa, Marie-Christine Maurel, Etienne Giraud, Françoise Culard, Bertrand Castaing, Alain Roussel, Axel Cloeckaert

Antimicrobial Agents and Chemotherapy 56:942-8 (2012)10.1128/AAC.05444-11

2012
Crystal structure of Diedel, a marker of the immune response of Drosophila melanogaster.

Franck Coste, Cordula Kemp, Vanessa Bobezeau, Charles Hetru, Christine Kellenberger, Jean-Luc Imler, Alain Roussel

PLoS ONE 7:e33416 (2012)10.1371/journal.pone.0033416

2012
Crystal structure of greglin, a novel non-classical Kazal inhibitor, in complex with subtilisin.

Chrystelle Derache, Christophe Epinette, Alain Roussel, Guillaume Gabant, Martine Cadene, Brice Korkmaz, Francis Gauthier, Christine Kellenberger

FEBS Journal 279:4466-78 (2012)10.1111/febs.12033

2011

2011
The Drosophila peptidoglycan-recognition protein LF interacts with peptidoglycan-recognition protein LC to downregulate the Imd pathway

Nada Basbous, Franck Coste, Philippe Leone, Renaud Vincentelli, Julien Royet, Christine Kellenberger, Alain Roussel

EMBO Reports 12:327-333 (2011)10.1038/embor.2011.19

2011
Structure-Function Analysis of Grass Clip Serine Protease Involved in Drosophila Toll Pathway Activation

Christine Kellenberger, Philippe Leone, Laurent Coquet, Thierry Jouenne, Jean-Marc Reichhart, Alain Roussel

Journal of Biological Chemistry 286:12300-12307 (2011)10.1074/jbc.M110.182741

2009

2009
Expression, purification, crystallization and preliminary X-ray analysis of the N-terminal domain of GNBP3 from Drosophila melanogaster

Yumiko Mishima, Franck Coste, Vanessa Bobezeau, Nadège Hervouet, Christine Kellenberger, Alain Roussel

Acta crystallographica Section F : Structural biology communications [2014-...] 65:870-873 (2009)10.1107/S1744309109014997

2009
The N-terminal Domain of Drosophila Gram-negative Binding Protein 3 (GNBP3) Defines a Novel Family of Fungal Pattern Recognition Receptors

Yumiko Mishima, Jessica Quintin, Vishukumar Aimanianda, Christine Kellenberger, Franck Coste, Cécile Clavaud, A. Hoffmann Jules, Jean-Paul Latgé, Dominique Ferrandon, Alain Roussel

Journal of Biological Chemistry 284:28687-28697 (2009)10.1074/jbc.M109.034587

2008

2008
Crystal structure of Drosophila PGRP-SD suggests binding to DAP-type but not lysine-type peptidoglycan.

P. Leone, V. Bischoff, C. Kellenberger, C. Hetru, J. Royet, A. Roussel

Molecular Immunology 45:2521-2530 (2008)

2008
Crystal structure of Drosophila PGRP-SD suggests binding to DAP-type but not lysine-type peptidoglycan

Philippe Leone, Vincent Bischoff, Christine Kellenberger, Charles Hetru, Julien Royetd, Alain Roussel

Molecular Immunology 45:2521-2530 (2008)10.1016/j.molimm.2008.01.015

2008
Structure of Locusta migratoria protease inhibitor 3 (LMPI-3) in complex with Fusarium oxysporum trypsin

Philippe Leone, Alain Roussel, Christine Kellenberger

Acta crystallographica Section D : Structural biology [1993-...] 64:1165-1171 (2008)10.1107/S0907444908030400

2007

2007
How much can a T-cell antigen receptor adapt to structurally distinct antigenic peptides?

Catherine Mazza, Nathalie Auphan-Anezin, Claude Gregoire, Annick Guimezanes, Christine Kellenberger, Alain Roussel, Alice Kearney, P Anton van Der Merwe, Anne-Marie Schmitt-Verhulst, Bernard Malissen

EMBO Journal 26:1972-83 (2007)10.1038/sj.emboj.7601605

2003

2003
Selective Inhibition of Trypsins by Insect Peptides: Role of P6−P10 Loop

Philippe Leone, C. Kellenberger, G. Ferrat, H. Darbon, A. Roussel

Biochemistry 42:13605-13612 (2003)10.1021/bi035318t

2002

2002
A medium-throughput crystallization approach

Gerlind Sulzenbacher, Arnaud Gruez, Véronique Roig-Zamboni, Silvia Spinelli, Christel Valencia, Fabienne Pagot, Renaud Vincentelli, Christophe Bignon, Aurelia Salomoni, Sacha Grisel, Damien Maurin, Céline Huyghe, Kent Johansson, Alice Grassick, Alain Roussel, Yves Bourne, Sophie Perrier, Linda Miallau, Phillippe Cantau, Eric Blanc, Michel Genevois, Alain Grossi, André Zenatti, Valérie Campanacci, Christian Cambillau

Acta crystallographica Section D : Structural biology [1993-...] 58:2109-2115 (2002)10.1107/s0907444902013938

2001

2001
The fusion glycoprotein shell from Semliki Forest Virus: an icosahedral assembly primed for fusogenic activation at endosomal pH

J. Lescar, A. Roussel, M.W. Wien, J. Navaza, S.D. Fuller, G. Wengler, F.A. Rey

Cell 137-148 (2001)

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