Research group: Host-Pathogen Interaction

Publications

2022

2022
SARS-CoV-2 detection using a nanobody-functionalized voltammetric device

Quentin Pagneux, Alain Roussel, Hiba Saada, Christian Cambillau, Béatrice Amigues, Vincent Delauzun, Ilka Engelmann, Enagnon Kazali Alidjinou, Judith Ogiez, Anne Sophie Rolland, Emmanuel Faure, Julien Poissy, Alain Duhamel, Rabah Boukherroub, David Devos, Sabine Szunerits

Communications Medicine 2:56 (2022)10.1038/s43856-022-00113-8

2022
[Review] The role of the surface ligand on the performance of electrochemical SARS-CoV-2 antigen biosensors

Sabine Szunerits, Quentin Pagneux, Abir Swaidan, Vladyslav Mishyn, Alain Roussel, Christian Cambillau, David Devos, Ilka Engelmann, Enagnon Kazali Alidjinou, Henri Happy, Rabah Boukherroub

Analytical and Bioanalytical Chemistry 414:pp 103-113 (2022)10.1007/s00216-020-03137-y

2021

2021
Preanalytical issues and cycle threshold values in SARS-CoV-2 real-time RT-PCR testing: should test results include these?

Ilka Engelmann, Enagnon Kazali Alidjinou, Judith Ogiez, Quentin Pagneux, Sana Miloudi, Ilyes Benhalima, Mahdi Ouafi, Famara Sané, Didier Hober, Alain Roussel, Christian Cambillau, David Devos, Rabah Boukherroub, Sabine Szunerits

ACS Omega 6:6528-6536 (2021)10.1021/acsomega.1c00166

2021
Anchoring the T6SS to the Cell Wall: Crystal Structure of the Peptidoglycan Binding Domain of the TagL Accessory Protein

Van Son Nguyen, Silvia Spinelli, Éric Cascales, Alain Roussel, Christian Cambillau, Philippe Leone

PLoS ONE (2021)

2021
Anchoring the T6SS to the cell wall: Crystal structure of the peptidoglycan binding domain of the TagL accessory protein

van Son Nguyen, Silvia Spinelli, E. Cascales, Alain Roussel, Christian Cambillau, Philippe Leone

PLoS ONE 16:e0254232 (2021)10.1371/journal.pone.0254232

2021
Crystal structures of two camelid nanobodies raised against GldL, a component of the Type IX Secretion System from Flavobacterium johnsoniae

Thi Trang Nhung, Anaïs Gaubert, Pauline Melani, Christian Cambillau, Alain Roussel, Philippe Leone

Acta crystallographica Section F : Structural biology communications [2014-...] (2021)

2021
Biogenesis of a Bacteriophage Long Non-Contractile Tail

Anait Seul, Sandrine Brasilès, Isabelle Petitpas, Rudi Lurz, Valérie Campanacci, Christian Cambillau, Frank Weise, Mohamed Zairi, Paulo Tavares, Isabelle Auzat

Journal of Molecular Biology 433:167112 (2021)10.1016/j.jmb.2021.167112

2020

2020
Revisiting the host adhesion determinants of Streptococcus thermophilus siphophages

Katherine Lavelle, Adeline Goulet, Brian Mcdonnell, Silvia Spinelli, Douwe Van Sinderen, Jennifer Mahony, Christian Cambillau

Microbial Biotechnology 13:1765-1779 (2020)10.1111/1751-7915.13593

2020
The CWPS Rubik’s cube: Linking diversity of cell wall polysaccharide structures with the encoded biosynthetic machinery of selected Lactococcus lactis strains

Jennifer Mahony, Cyril Frantzen, Evgeny Vinogradov, Irina Sadovskaya, Ilias Theodorou, Philip Kelleher, Marie‐pierre Chapot-Chartier, Christian Cambillau, Helge Holo, Douwe Sinderen

Molecular Microbiology 114:582-596 (2020)10.1111/mmi.14561

2020
Novel Genus of Phages Infecting Streptococcus thermophilus : Genomic and Morphological Characterization

Cécile Philippe, Sébastien Levesque, Moïra Dion, Denise Tremblay, Philippe Horvath, Natascha Lüth, Christian Cambillau, Charles Franz, Horst Neve, Christophe Fremaux, Knut Heller, Sylvain Moineau

Applied and Environmental Microbiology 86 (2020)10.1128/AEM.00227-20

2020
Crystal structure of Type IX secretion system PorE C-terminal domain from Porphyromonas gingivalis in complex with a peptidoglycan fragment

Nhung Thi Trang Trinh, Hieu Quang Tran, Quyen van Dong, Christian Cambillau, Alain Roussel, Philippe Leone

Scientific Reports 10 (2020)10.1038/s41598-020-64115-z

2019

2019
Cas9 Allosteric Inhibition by the Anti-CRISPR Protein AcrIIA6

Olivier Fuchsbauer, Paolo Swuec, Claire Zimberger, Beatrice Amigues, Sébastien Levesque, Daniel Agudelo, Alexis Duringer, Antonio Chaves-Sanjuan, Silvia Spinelli, Geneviève Rousseau, Minja Velimirovic, Martino Bolognesi, Alain Roussel, Christian Cambillau, Sylvain Moineau, Yannick Doyon, Adeline Goulet

Molecular Cell 76:922-937.e7 (2019)10.1016/j.molcel.2019.09.012

2019
Ubiquitous Carbohydrate Binding Modules Decorate 936 Lactococcal Siphophage Virions

Stephen Hayes, Jennifer Mahony, Renaud Vincentelli, Laurie Ramond, Arjen Nauta, Douwe van Sinderen, Christian Cambillau

Viruses 11:631 (2019)10.3390/v11070631

2018

2018
ANISEED 2017: extending the integrated ascidian database to the exploration and evolutionary comparison of genome-scale datasets

Matija Brozovic, Christelle Dantec, Justine Dardaillon, Delphine Dauga, Emmanuel Faure, Mathieu Gineste, Alexandra Louis, Magali Naville, Kazuhiro R. Nitta, Jacques Piette, Wendy Reeves, Celine Scornavacca, Paul Simion, Renaud Vincentelli, Maëlle Bellec, Sameh Ben Aicha, Marie Fagotto, Marion Gueroult-Bellone, Maximilian Haeussler, Edwin Jacox, Elijah K. Lowe, Mickaël Mendez, Alexis Roberge, Alberto Stolfi, Rui Yokomori, C. Titus Brown, Christian Cambillau, Lionel Christiaen, Frédéric Delsuc, Emmanuel J.P. Douzery, Rémi Dumollard, Takehiro Kusakabe, Kenta Nakai, Hiroki Nishida, Yutaka Satou, Billie Swalla, Michael Veeman, Jean-Nicolas Volff, Patrick Lemaire

Nucleic Acids Research 46:D718-D725 (2018)10.1093/nar/gkx1108

2018
Structure–Function Analysis of the C-Terminal Domain of the Type VI Secretion TssB Tail Sheath Subunit

Badreddine Douzi, Laureen Logger, Silvia Spinelli, Stephanie Blangy, Christian Cambillau, E. Cascales

Journal of Molecular Biology 430:297-309 (2018)10.1016/j.jmb.2017.11.015

2018
Functional carbohydrate binding modules identified in evolved dits from siphophages infecting various Gram-positive bacteria

Stephen Hayes, Renaud Vincentelli, Jennifer Mahony, Arjen Nauta, Laurie Ramond, Gabriele Lugli, Marco Ventura, Douwe van Sinderen, Christian Cambillau

Molecular Microbiology 110:777-795 (2018)10.1111/mmi.14124

2018
Identification of Dual Receptor Binding Protein Systems in Lactococcal 936 Group Phages

Stephen Hayes, Yoan Duhoo, Horst H. Neve, James Murphy, Jean-Paul Noben, Charles M. A. P. Franz, Christian Cambillau, Jennifer Mahony, Arjen Nauta, Douwe van Sinderen

Viruses 10:668 (2018)10.3390/v10120668

2018
Widespread anti-CRISPR proteins in virulent bacteriophages inhibit a range of Cas9 proteins

Alexander Hynes, Geneviève Rousseau, Daniel Agudelo, Adeline Goulet, Béatrice Amigues, Jeremy Loehr, Dennis Romero, Christophe Fremaux, Philippe Horvath, Yannick Doyon, Christian Cambillau, Sylvain S. Moineau

Nature Communications 9:2919 (2018)10.1038/s41467-018-05092-w

2018
A Decade of Streptococcus thermophilus Phage Evolution in an Irish Dairy Plant

Katherine Lavelle, James Murphy, Brian Fitzgerald, Gabriele Lugli, Aldert Zomer, Horst H. Neve, Marco Ventura, Charles M. A. P. Franz, Christian Cambillau, Douwe van Sinderen, Jennifer Mahony

Applied and Environmental Microbiology 84 (2018)10.1128/AEM.02855-17

2018
Towards a complete structural deciphering of Type VI secretion system

van Son Nguyen, Badreddine Douzi, Eric Durand, Alain Roussel, E. Cascales, Christian Cambillau

Current Opinion in Structural Biology 49:77-84 (2018)10.1016/j.sbi.2018.01.007

2018
Structure of the type VI secretion system TssK–TssF–TssG baseplate subcomplex revealed by cryo-electron microscopy

Young-Jun Park, Kaitlyn Lacourse, Christian Cambillau, Frank Dimaio, Joseph D. Mougous, David Veesler

Nature Communications 9:5385 (2018)10.1038/s41467-018-07796-5

2018
The gp27-like Hub of VgrG Serves as Adaptor to Promote Hcp Tube Assembly

Melvin G Renault, Jordi Zamarreño Beas, Badreddine Douzi, Maïalène Chabalier, Abdelrahim Zoued, Yannick R Brunet, Christian Cambillau, Laure Journet, E. Cascales

Journal of Molecular Biology 430:3143-3156 (2018)10.1016/j.jmb.2018.07.018

2017

2017
Bivalent Llama Single-Domain Antibody Fragments against Tumor Necrosis Factor Have Picomolar Potencies due to Intramolecular Interactions

Els Beirnaert, Aline Desmyter, Silvia Spinelli, Marc Lauwereys, Lucien Aarden, Torsten Dreier, Remy Loris, Karen Silence, Caroline Pollet, Christian Cambillau, Hans de Haard

Frontiers in Immunology 8:867 (2017)10.3389/fimmu.2017.00867

2017
Neutralization of Human Interleukin 23 by Multivalent Nanobodies Explained by the Structure of Cytokine–Nanobody Complex

Aline Desmyter, Silvia Spinelli, Carlo Boutton, Michael Saunders, Christophe Blachetot, Hans de Haard, Geertrui Denecker, Maarten van Roy, Christian Cambillau, Heidi Rommelaere

Frontiers in Immunology 8:884 (2017)10.3389/fimmu.2017.00884

2017
Evolved distal tail carbohydrate binding modules of L actobacillus phage J-1: a novel type of anti-receptor widespread among lactic acid bacteria phages

Maria-Eugenia Dieterle, Silvia Spinelli, Irina I. Sadovskaya, Mariana Piuri, Christian Cambillau

Molecular Microbiology 104:608-620 (2017)10.1111/mmi.13649

2017
Unraveling the Self-Assembly of the Pseudomonas aeruginosa XcpQ Secretin Periplasmic Domain Provides New Molecular Insights into Type II Secretion System Secreton Architecture and Dynamics

Badreddine Douzi, Nhung Trinh, Sandra Michel-Souzy, Aline Desmyter, Genevieve Ball, Pascale Barbier, Artemis Kosta, Eric Durand, Katrina Forest, Christian Cambillau, Alain Roussel, Romé Voulhoux

mBio 8 (2017)10.1128/mBio.01185-17

2017
Camelid nanobodies used as crystallization chaperones for different constructs of PorM, a component of the type IX secretion system from Porphyromonas gingivalis

Yoan Duhoo, Jennifer Roche, Thi Trang Nhung Trinh, Aline Desmyter, Anaïs Gaubert, Christine Kellenberger, Christian Cambillau, Alain Roussel, Philippe Leone

Acta crystallographica Section F : Structural biology communications [2014-...] 73:286-293 (2017)10.1107/S2053230X17005969

2017
Genetic and functional characterisation of the lactococcal P335 phage-host interactions

Jennifer Mahony, Joana Oliveira, Barry Collins, Laurens Hanemaaijer, Gabriele Andrea Lugli, Horst H. Neve, Marco Ventura, Thijs Kouwen, Christian Cambillau, Douwe van Sinderen

BMC Genomics 18:146 (2017)10.1186/s12864-017-3537-5

2017
Host recognition by lactic acid bacterial phages

Jennifer Mahony, Christian Cambillau, Douwe van Sinderen

FEMS Microbiology Reviews 41:S16-S26 (2017)10.1093/femsre/fux019

2017
Type VI secretion TssK baseplate protein exhibits structural similarity with phage receptor-binding proteins and evolved to bind the membrane complex

van Son Nguyen, Laureen Logger, Silvia Spinelli, Pierre Legrand, Thi Thanh Huyen Pham, Thi Trang Nhung Trinh, Yassine Cherrak, Abdelrahim Zoued, Aline Desmyter, Eric Durand, Alain Roussel, Christine Kellenberger, E. Cascales, Christian Cambillau

Nature Microbiology 2:17103 (2017)10.1038/nmicrobiol.2017.103

2017
Characterization of the Porphyromonas gingivalis Type IX Secretion Trans-envelope PorKLMNP Core Complex

Maxence S Vincent, Mickaël J Canestrari, Philippe Leone, Julien Stathopulos, Bérengère Ize, Abdelrahim Zoued, Christian Cambillau, Christine Kellenberger, Alain Roussel, E. Cascales

Journal of Biological Chemistry 292:3252-3261 (2017)10.1074/jbc.M116.765081

2017
Reverse chemical ecology: Olfactory proteins from the giant panda and their interactions with putative pheromones and bamboo volatiles

Jiao Zhu, Simona Arena, Silvia Spinelli, Dingzhen Liu, Guiquan Zhang, Rongping Wei, Christian Cambillau, Andrea Scaloni, Guirong Wang, Paolo Pelosi

Proceedings of the National Academy of Sciences of the United States of America 114:E9802-E9810 (2017)10.1073/pnas.1711437114

2017
TssA: The cap protein of the Type VI secretion system tail

Abdelrahim Zoued, Eric Durand, Yoann G Santin, Laure Journet, Alain Roussel, Christian Cambillau, E. Cascales

BioEssays 39 (2017)10.1002/bies.201600262

2016

2016
The Baseplate of Lactobacillus delbrueckii Bacteriophage Ld17 Harbors a Glycerophosphodiesterase

Anneleen Cornelissen, Irina Sadovskaya, Evgeny Vinogradov, Stephanie Blangy, Silvia Spinelli, Eoghan Casey, Jennifer Mahony, Jean-Paul Noben, Fabio Dal Bello, Christian Cambillau, Douwe Van Sinderen

Journal of Biological Chemistry 291:16816+ (2016)10.1074/jbc.M116.728279

2016
Characterization of prophages containing "evolved" Dit/Tal modules in the genome of Lactobacillus casei BL23.

María Eugenia Dieterle, Joaquina Fina Martin, Rosario Durán, Sergio I Nemirovsky, Carmen Sanchez Rivas, Charles Bowman, Daniel Russell, Graham F Hatfull, Christian Cambillau, Mariana Piuri

Applied Microbiology and Biotechnology 100:9201-9215 (2016)10.1007/s00253-016-7727-x

2016
Structure and specificity of the Type VI secretion system ClpV-TssC interaction in enteroaggregative Escherichia coli

Badreddine Douzi, Yannick R Brunet, Silvia Spinelli, Valentine Lensi, Stephanie Blangy, Anant Kumar, Laure Journet, Pierre Legrand, E. Cascales, Christian Cambillau

Scientific Reports 6 (2016)10.1038/srep34405

2016
A phospholipase A 1 antibacterial Type VI secretion effector interacts directly with the C-terminal domain of the VgrG spike protein for delivery

Nicolas Flaugnatti, Thi Thu Hang Le, Stéphane Canaan, Marie-Stéphanie Aschtgen, Vân Nguyên, Stephanie Blangy, Christine Kellenberger, Alain Roussel, Christian Cambillau, E. Cascales, Laure Journet

Molecular Microbiology 99:1099-1118 (2016)10.1111/mmi.13292

2016
Structural Mimicry of Receptor Interaction by Antagonistic Interleukin-6 (IL-6) Antibodies

Anna Hultberg, Anke Kretz-Rommel, Silvia Spinelli, Remy Loris, Christian Cambillau, Hans De Haard, Christophe Blanchetot, Natalie de Jonge, Aline Desmyter, Nico Ongenae, Erik Hofman, Alex Klarenbeek, Ava Sadi

Journal of Biological Chemistry 291:13846-13854 (2016)10.1074/jbc.M115.695528

2016
Combining somatic mutations present in different in vivo affinity-matured antibodies isolated from immunized Lama glama yields ultra-potent antibody therapeutics

Alex Klarenbeek, Christophe Blanchetot, Georg Schragel, Ava S. Sadi, Nico Ongenae, Wieger Hemrika, John Wijdenes, Silvia Spinelli, Aline Desmyter, Christian Cambillau, Anna Hultberg, Anke Kretz-Rommel, Torsten Dreier, Hans J. W. de Haard, Rob C. Roovers

Protein Engineering, Design and Selection 29:123-133 (2016)10.1093/protein/gzw003

2016
Structure of the host-recognition device of Staphylococcus aureus phage phi 11

Cengiz Koç, Guoqing Xia, Petra Kuehner, Silvia Spinelli, Alain Roussel, Christian Cambillau, Thilo Stehle

Scientific Reports 6 (2016)10.1038/srep27581

2016
The Atomic Structure of the Phage Tuc2009 Baseplate Tripod Suggests that Host Recognition Involves Two Different Carbohydrate Binding Modules

Pierre Legrand, Barry Collins, Stephanie Blangy, James Murphy, Silvia Spinelli, Carlos Gutierrez, Nicolas Richet, Christine Kellenberger, Aline Desmyter, Jennifer Mahony, Douwe Van Sinderen, Christian Cambillau

mBio 7 (2016)10.1128/mBio.01781-15

2016
An essential role for the baseplate protein Gp45 in phage adsorption to Staphylococcus aureus

Xuehua Li, Cengiz Koc, Petra Kuehner, York-Dieter Stierhof, Bernhard Krismer, Mark C. Enright, Jose R. Penades, Christiane Wolz, Thilo Stehle, Christian Cambillau, Andreas Peschel, Guoqing Xia

Scientific Reports 6 (2016)10.1038/srep26455

2016
Functional and structural dissection of the tape measure protein of lactococcal phage TP901-1

Jennifer Mahony, Mona Alqarni, Stephen Stockdale, Silvia Spinelli, Marine Feyereisen, Christian Cambillau, Douwe Van Sinderen

Scientific Reports 6 (2016)10.1038/srep36667

2016
Priming and polymerization of a bacterial contractile tail structure. 1" 2" 17

Abdelrahim Zoued, Eric Durand, Yannick R Brunet, Silvia Spinelli, Badreddine Douzi, Mathilde Guzzo, Nicolas Flaugnatti, Pierre Legrand, Laure Journet, Rémi Fronzes, Tam Mignot, Christian Cambillau, E. Cascales

Nature 531:59-63 (2016)10.1038/nature17182

2016
Structure–Function Analysis of the TssL Cytoplasmic Domain Reveals a New Interaction between the Type VI Secretion Baseplate and Membrane Complexes

Abdelrahim Zoued, Chloé J Cassaro, Eric Durand, Badreddine Douzi, Alexandre España, Christian Cambillau, Laure Journet, E. Cascales

Journal of Molecular Biology 428:4413-4423 (2016)10.1016/j.jmb.2016.08.030

2015

2015
Camelid nanobodies: killing two birds with one stone

Aline Desmyter, Silvia Spinelli, Alain Roussel, Christian Cambillau

Current Opinion in Structural Biology 32:1-8 (2015)10.1016/j.sbi.2015.01.001

2015
Camelid Ig V genes reveal significant human homology not seen in therapeutic target genes, providing for a powerful therapeutic antibody platform

Alex Klarenbeek, Khalil El Mazouari, Aline Desmyter, Christophe Blanchetot, Anna Hultberg, Natalie De Jonge, Rob C. Roovers, Christian Cambillau, Sylvia Spinelli, Jurgen Del-Favero, Theo Verrips, Hans J. De Haard, Ikbel Achour

mAbs 7:693-706 (2015)10.1080/19420862.2015.1046648

2015
X-ray and Cryo-electron Microscopy Structures of Monalysin Pore-forming Toxin Reveal Multimerization of the Pro-form

Philippe Leone, Cecilia Bebeacua, Onya Opota, Christine Kellenberger, Bruno Klaholz, Igor Orlov, Christian Cambillau, Bruno Lemaitre, Alain Roussel

Journal of Biological Chemistry 290:13191-13201 (2015)10.1074/jbc.M115.646109

2015
The targeted recognition of Lactococcus lactis phages to their polysaccharide receptors

Orla Mccabe, Silvia Spinelli, Carine Farenc, Myriam Labbe, Denise Tremblay, Stephanie Blangy, Stefan Oscarson, Sylvain Moineau, Christian Cambillau

Molecular Microbiology 96:875-886 (2015)10.1111/mmi.12978

2015
Inhibition of Type VI Secretion by an Anti-TssM Llama Nanobody

van Son Nguyen, Laureen Logger, Silvia Spinelli, Aline Desmyter, Thi Thu Hang Le, Christine Kellenberger, Badreddine Douzi, Eric Durand, Alain Roussel, E. Cascales, Christian Cambillau

PLoS ONE 10:1-14 (2015)

2015
Production, crystallization and X-ray diffraction analysis of a complex between a fragment of the TssM T6SS protein and a camelid nanobody

van Son Nguyen, Silvia Spinelli, Aline Desmyter, Thi Thu Hang Le, Christine Kellenberger, E. Cascales, Christian Cambillau, Alain Roussel

Acta crystallographica Section F : Structural biology communications [2014-...] 71:266-271 (2015)10.1107/S2053230X15000709

2015
Crystallization and preliminary X-ray analysis of the C-terminal fragment of PorM, a subunit of the Porphyromonas gingivalis type IX secretion system

Julien Stathopulos, Christian Cambillau, E. Cascales, Alain Roussel, Philippe Leone

Acta crystallographica Section F : Structural biology communications [2014-...] 71:71-74 (2015)10.1107/S2053230X1402559X

2015
Structure and Assembly of TP901-1 Virion Unveiled by Mutagenesis

Stephen R. Stockdale, Barry Collins, Silvia Spinelli, François P. Douillard, Jennifer Mahony, Christian Cambillau, Douwe van Sinderen

PLoS ONE 1-22 (2015)10.1371/journal.pone.0131676

2014

2014
Differences in lactococcal cell wall polysaccharide structure are major determining factors in bacteriophage sensitivity

Stuart Ainsworth, Irina Sadovskaya, Evguenii Vinogradov, Pascal Courtin, Yann Guerardel, Jennifer Mahony, Thierry Grard, Christian Cambillau, Marie-Pierre Chapot-Chartier, Douwe van Sinderen

mBio 5:np (2014)10.1128/mBio.00880-14

2014
Crystal Structure and Self-Interaction of the Type VI Secretion Tail-Tube Protein from Enteroaggregative Escherichia coli

Badreddine Douzi, Silvia Spinelli, Stéphanie Blangy, Alain Roussel, Eric Durand, Yannick Brunet, E. Cascales, Christian Cambillau

PLoS ONE 9:e86918 (2014)10.1371/journal.pone.0086918

2014
VgrG, Tae, Tle, and beyond: the versatile arsenal of Type VI secretion effectors.

Eric Durand, Christian Cambillau, E. Cascales, Laure Journet

Trends Microbiol. 22:498-507 (2014)10.1016/j.tim.2014.06.004

2014
VgrG, Tae, Tle, and beyond: the versatile arsenal of Type VI secretion effectors

Eric Durand, Christian Cambillau, E. Cascales, Laure Journet

Trends in Microbiology 22:498-507 (2014)10.1016/j.tim.2014.06.004

2014
A Cutinase from Trichoderma reesei with a Lid-Covered Active Site and Kinetic Properties of True Lipases

Alain Roussel, Sawsan Amara, Antti Nyyssölä, Eduardo Mateos-Diaz, Stéphanie Blangy, Hanna Kontkanen, Ann Westerholm-Parvinen, Frédéric Carrière, Christian Cambillau

Journal of Molecular Biology 426:3757-3772 (2014)10.1016/j.jmb.2014.09.003

2014
Architecture and assembly of the Type VI secretion system

Abdelrahim Zoued, Yannick Brunet, Eric Durand, Marie-Stéphanie Aschtgen, Laureen Logger, Badreddine Douzi, Laure Journet, Christian Cambillau, E. Cascales

Biochimica et Biophysica Acta - Molecular Cell Research 1843:1664-1673 (2014)10.1016/j.bbamcr.2014.03.018

2014
Architecture and assembly of the Type VI secretion system.

Abdelrahim Zoued, Yannick R Brunet, Eric Durand, Marie-Stéphanie Aschtgen, Laureen Logger, Badreddine Douzi, Laure Journet, Christian Cambillau, E. Cascales

Biochim. Biophys. Acta 1843:1664-73 (2014)10.1016/j.bbamcr.2014.03.018

2013

2013
Biochemical and structural characterization of non-glycosylated Yarrowia lipolytica LIP2 lipase

Ahmed Aloulou, Anaïs Bénarouche, Delphine Puccinelli, Silvia Spinelli, Jean-François Cavalier, Christian Cambillau, Frédéric Carrière

European Journal of Lipid Science and Technology 115:429-441 (2013)10.1002/ejlt.201200440

2013
X-ray structure of a superinfection exclusion lipoprotein from phage TP-J34 and identification of the tape measure protein as its target

Cecilia C. Bebeacua, Juan Carlos Lorenzo Fajardo, Stéphanie Blangy, Silvia Spinelli, Stefanie Bollmann, Horst H. Neve, Christian Cambillau, Knut Heller

Molecular Microbiology 89:152-165 (2013)10.1111/mmi.12267

2013
Dissection of the TssB-TssC Interface during Type VI Secretion Sheath Complex Formation

Xiang Y. Zhang, Yannick R Brunet, Laureen Logger, Badreddine Douzi, Christian Cambillau, Laure Journet, E. Cascales

PLoS ONE 8:e81074 (2013)10.1371/journal.pone.0081074

2013
TssK Is a Trimeric Cytoplasmic Protein Interacting with Components of Both Phage-like and Membrane Anchoring Complexes of the Type VI Secretion System

Abdelrahim Zoued, Eric Durand, Cecilia C. Bebeacua, Yannick R Brunet, Badreddine Douzi, Christian Cambillau, E. Cascales, Laure Journet

Journal of Biological Chemistry 288:27031-27041 (2013)10.1074/jbc.M113.499772

2012

2012
Structural biology of type VI secretion systems

E. Cascales, Christian Cambillau

Philosophical Transactions of the Royal Society B: Biological Sciences 367:1102-1111 (2012)10.1098/rstb.2011.0209

2012
Structural biology of type VI secretion systems.

E. Cascales, Christian Cambillau

Philos. Trans. R. Soc. Lond., B, Biol. Sci. 367:1102-11 (2012)10.1098/rstb.2011.0209

2012
Crystal structure of the VgrG1 actin cross-linking domain of the Vibrio cholerae type VI secretion system.

Eric Durand, Estelle Derrez, Gilles Audoly, Silvia Spinelli, Miguel Ortiz-Lombardia, Didier Raoult, E. Cascales, Christian Cambillau

Journal of Biological Chemistry 287:38190-9 (2012)10.1074/jbc.M112.390153

2012
Structural characterization and oligomerization of the TssL protein, a component shared by bacterial type VI and type IVb secretion systems.

Eric Durand, Abdelrahim Zoued, Silvia Spinelli, Paul J H Watson, Marie-Stéphanie Aschtgen, Laure Journet, Christian Cambillau, E. Cascales

Journal of Biological Chemistry 287:14157-68 (2012)10.1074/jbc.M111.338731

2012
Crystal Structure of the VgrG1 Actin Cross-linking Domain of the Vibrio cholerae Type VI Secretion System

Eric Durand, Estelle Derrez, Gilles Audoly, Silvia Spinelli, Miguel Ortiz-Lombardia, Didier Raoult, E. Cascales, Christian Cambillau

Journal of Biological Chemistry 287:38190-38199 (2012)10.1074/jbc.M112.390153

2012
Crystal Structure of ATVORF273, a New Fold for a Thermo- and Acido-Stable Protein from the Acidianus Two-Tailed Virus

Catarina Felisberto-Rodrigues, Stéphanie Blangy, Adeline Goulet, Gisle Vestergaard, Christian Cambillau, Roger Garrett, Miguel Ortiz-Lombardía

PLoS ONE 7:e45847 (2012)10.1371/journal.pone.0045847

2011

2011
Deciphering the Xcp Pseudomonas aeruginosa type II secretion machinery through multiple interactions with substrates.

Badreddine Douzi, Geneviève Ball, Christian Cambillau, Mariella Tegoni, Romé Voulhoux

Journal of Biological Chemistry 286:40792-801 (2011)10.1074/jbc.M111.294843

2011
Towards a Structural Comprehension of Bacterial Type VI Secretion Systems: Characterization of the TssJ-TssM Complex of an Escherichia coli Pathovar

Catarina Felisberto-Rodrigues, Eric Durand, Marie-Stéphanie Aschtgen, Stéphanie Blangy, Miguel Ortiz-Lombardia, Badreddine Douzi, Christian Cambillau, E. Cascales

PLoS Pathogens 7:e1002386 (2011)10.1371/journal.ppat.1002386

2011
Towards a structural comprehension of bacterial type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar

Catarina Felisberto-Rodrigues, Eric Durand, Marie-Stéphanie Aschtgen, Stephanie Blangy, Miguel Ortiz-Lombardia, Badreddine Douzi, Christian Cambillau, E. Cascales

PLoS Pathogens 7:e1002386 (2011)10.1371/journal.ppat.1002386

2011
The Opening of the SPP1 Bacteriophage Tail, a Prevalent Mechanism in Gram-positive-infecting Siphophages

Adeline Goulet, Joséphine Lai-Kee-Him, David Veesler, Isabelle Auzat, Gautier Robin, Dale Shepherd, Alison Ashcroft, Eric Richard, Julie Lichière, Paulo Tavares, Christian Cambillau, Patrick Bron

Journal of Biological Chemistry 286:25397-25405 (2011)10.1074/jbc.M111.243360

2010

2010
Solution and electron microscopy characterization of lactococcal phage baseplates expressed in Escherichia coli.

Valérie Campanacci, David Veesler, Julie Lichière, Stéphanie Blangy, Giuliano Sciara, Sylvain Moineau, Douwe van Sinderen, Patrick Bron, Christian Cambillau

Journal of Structural Biology 172:75-84 (2010)10.1016/j.jsb.2010.02.007

2010
Mammalian G protein-coupled receptor expression in Escherichia coli: II. Refolding and biophysical characterization of mouse cannabinoid receptor 1 and human parathyroid hormone receptor 1.

Kerstin Michalke, Céline Huyghe, Julie Lichière, Marie-Eve Gravière, Marina Siponen, Giuliano Sciara, Isabelle Lepaul, Renaud Wagner, Christine Magg, Rainer Rudolph, Christian Cambillau, Aline Desmyter

Analytical Biochemistry 401:74-80 (2010)10.1016/j.ab.2010.02.017

2010
Structure of lactococcal phage p2 baseplate and its mechanism of activation.

Giuliano Sciara, Cecilia Bebeacua, Patrick Bron, Denise Tremblay, Miguel Ortiz-Lombardia, Julie Lichière, Marin van Heel, Valérie Campanacci, Sylvain Moineau, Christian Cambillau

Proceedings of the National Academy of Sciences of the United States of America 107:6852-7 (2010)10.1073/pnas.1000232107

2010
Crystal structure of Bacillus subtilis SPP1 phage gp22 shares fold similarity with a domain of lactococcal phage p2 RBP

David Veesler, Stéphanie Blangy, Silvia Spinelli, Paulo Tavares, Valérie Campanacci, Christian Cambillau

Protein Science 19:1439-1443 (2010)10.1002/pro.416

2010
Crystal structure of Bacillus subtilis SPP1 phage gp23.1, a putative chaperone

David Veesler, Stéphanie Blangy, Julie Lichiere, Miguel Ortiz-Lombardía, Paulo Tavares, Valérie Campanacci, Christian Cambillau

Protein Science 19:1812-1816 (2010)10.1002/pro.464

2009

2009
Surfactant Poloxamer 188 as a New Crystallizing Agent for Urate Oxidase

Marion Giffard, Vanessa Delfosse, Giuliano Sciara, Claudine Mayer, Christian Cambillau, Mohamed El Hajji, Bertrand Castro, Françoise Bonneté

Crystal Growth & Design 9:4199-4206 (2009)10.1021/cg900473n

2009
Acidianus filamentous virus 1 coat proteins display a helical fold spanning the filamentous archaeal viruses lineage.

Adeline Goulet, Stéphanie Blangy, Peter Redder, David Prangishvili, Catarina Felisberto-Rodrigues, Patrick Forterre, Valérie Campanacci, Christian Cambillau

Proceedings of the National Academy of Sciences of the United States of America 106:21155-60 (2009)10.1073/pnas.0909893106

2009
The crystal structure of ORF14 from Sulfolobus islandicus filamentous virus

Adeline Goulet, Silvia Spinelli, Stéphanie Blangy, Herman van Tilbeurgh, Nicolas Leulliot, Tamara Basta, David Prangishvili, Christian Cambillau, Valérie Campanacci

Proteins - Structure, Function and Bioinformatics 76:1020-1022 (2009)10.1002/prot.22448

2009
The thermo- and acido-stable ORF-99 from the archaeal virus AFV1

Adeline Goulet, Silvia Spinelli, Stéphanie Blangy, Herman van Tilbeurgh, Nicolas Leulliot, Tamara Basta, David Prangishvili, Christian Cambillau, Valérie Campanacci

Protein Science 18:1316-1320 (2009)10.1002/pro.122

2009
Mammalian G-protein-coupled receptor expression in Escherichia coli: I. High-throughput large-scale production as inclusion bodies

Kerstin Michalke, Marie-Eve Gravière, Céline Huyghe, Renaud Vincentelli, Renaud Wagner, Franc Pattus, Kathrin Schroeder, Jan Oschmann, Rainer Rudolph, Christian Cambillau, Aline Desmyter

Analytical Biochemistry 386:147-155 (2009)10.1016/j.ab.2008.12.016

2009
Queen bee pheromone binding protein pH-induced domain swapping favors pheromone release

Marion E. Pesenti, Silvia Spinelli, Valérie Bézirard, Loïc Briand, J Claude Pernollet, Valérie Campanacci, Mariella Tegoni, Christian Cambillau

Journal of Molecular Biology 390:981-990 (2009)10.1016/j.jmb.2009.05.067

2009
Crystal structure of ORF12 from Lactococcus lactis phage p2 identifies a tape measure protein chaperone.

Marina Siponen, Giuliano Sciara, Manuela Villion, Silvia Spinelli, Julie Lichière, Christian Cambillau, Sylvain Moineau, Valérie Campanacci

Journal of Bacteriology 191:728-34 (2009)10.1128/JB.01363-08

2009
Production and biophysical characterization of the CorA transporter from Methanosarcina mazei.

David Veesler, Stéphanie Blangy, Marina Siponen, Renaud Vincentelli, Christian Cambillau, Giuliano Sciara

Analytical Biochemistry 388:115-21 (2009)10.1016/j.ab.2009.02.018

2008

2008
Structure of Human Pancreatic Lipase-Related Protein 2 with the Lid in an Open Conformation ,

Cécilia Eydoux, Silvia Spinelli, Tara Davis, John Walker, Alma Seitova, Sirano Dhe-Paganon, Alain de Caro, Christian Cambillau, Frédéric Carrière

Biochemistry 47:9553-9564 (2008)10.1021/bi8005576

2008
Structural basis of the honey bee PBP pheromone and pH-induced conformational change

Marion E. Pesenti, Silvia Spinelli, Valérie Bézirard, Loïc Briand, J Claude Pernollet, Mariella Tegoni, Christian Cambillau

Journal of Molecular Biology 380:158-169 (2008)10.1016/j.jmb.2008.04.048

2008
A Topological Model of the Baseplate of Lactococcal Phage Tuc2009

Giuliano Sciara, Stéphanie Blangy, Marina I Siponen, Stephen Mc Grath, Douwe van Sinderen, Mariella Tegoni, Christian Cambillau, Valérie Campanacci

Journal of Biological Chemistry 283:2716-2723 (2008)10.1074/jbc.M707533200

2008
There is a baby in the bath water: AcrB contamination is a major problem in membrane-protein crystallization.

David Veesler, Stéphanie Blangy, Christian Cambillau, Giuliano Sciara

Acta Crystallographica Section F: Structural Biology and Crystallization Communications 64:880-885 (2008)10.1107/S1744309108028248

2007

2007
Crystal structure of AFV3-109, a highly conserved protein from crenarchaeal viruses.

Jenny Keller, Nicolas Leulliot, Christian Cambillau, Valérie Campanacci, Stéphanie Porciero, David Prangishvili, Patrick Forterre, Diego Cortez, Sophie Quevillon-Cheruel, Herman van Tilbeurgh

Virology 4:12 (2007)10.1186/1743-422X-4-12

2007
ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome

Michael J. Taussing, Oda Stoevesandt, Carl a K Borrebaeck, Andrew R Bradbury, Dolores Cahill, Christian Cambillau, Antoine de Daruvar, Stefan Dübel, Jutta Eichler, Ronald Frank, Toby J. Gibson, David Gloriam, Larry Gold, Friedrich W. Herberg, Henning Hermjakob, Jörg D. Hoheisel, Thomas O. Joos, Olli Kallioniemi, Manfred Koegl, Zoltán Konthur, Bernhard Korn, Elisabeth Kremmer, Sylvia Krobitsch, Ulf Landegren, Silvère van Der Maarel, Serge Muyldermans, John Mccafferty, Per-Åke Nygren, Sandrine Palcy, Andreas Plückthun, Bojan Polic, Michael Przybylski, Petri Saviranta, Alan Sawyer, David James Sherman, Arne Skerra, Markus Templin, Marius Ueffing, Mathias Uhlén

Nature Methods 4:13-17 (2007)10.1038/nmeth0107-13

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