Research group: Lipolysis & Bacterial PathogenicityGroup leader

Phone: +33 (0)4 91 16 40 93

Room: BM311

Publications

2021

2021
Intrabacterial lipid inclusions in mycobacteria: unexpected key players in survival and pathogenesis?

Ivy Mallick, Pierre Santucci, Isabelle Poncin, Vanessa Point, Laurent Kremer, Jean-François Cavalier, Stéphane Canaan

FEMS Microbiology Reviews (2021)10.1093/femsre/fuab029

2021
Transcriptional adaptation of Mycobacterium ulcerans in an original mouse model: New insights into the regulation of mycolactone

Marie Robbe-Saule, Mélanie Foulon, Isabelle Poncin, Lucille Esnault, Hugo Varet, Rachel Legendre, Alban Besnard, Anna Grzegorzewicz, Mary Jackson, Stéphane Canaan, Laurent Marsollier, Estelle Marion

Virulence 12:1438-1451 (2021)10.1080/21505594.2021.1929749

2020

2020
Lipolytic enzymes inhibitors: A new way for antibacterial drugs discovery

Jean-François Cavalier, Christopher Spilling, Thierry Durand, Luc Camoin, Stéphane Canaan

European Journal of Medicinal Chemistry 112908 (2020)10.1016/j.ejmech.2020.112908

2020
Structural basis for loading and inhibition of a bacterial T6 SS phospholipase effector by the VgrG spike

Nicolas Flaugnatti, Chiara Rapisarda, Martial Rey, Solène Beauvois, Viet Anh Nguyen, Stéphane Canaan, Eric Durand, Julia Chamot‐rooke, E. Cascales, Rémi Fronzes, Laure Journet

EMBO Journal 39:e104129 (2020)10.15252/embj.2019104129

2020
A TLR2-Activating Fraction From Mycobacterium abscessus Rough Variant Demonstrates Vaccine and Diagnostic Potential

Vincent Le Moigne, Anne-Laure Roux, Aude Jobart-Malfait, Landry Blanc, Karima Chaoui, Odile Burlet-Schiltz, Jean-Louis Gaillard, Stéphane Canaan, Jérôme Nigou, Jean-Louis Herrmann

Frontiers in Cellular and Infection Microbiology 10 (2020)10.3389/fcimb.2020.00432

2020
Dissecting the antibacterial activity of oxadiazolone-core derivatives against Mycobacterium abscessus

Abdeldjalil Madani, Ivy Mallick, Alexandre Guy, Céline Crauste, Thierry Durand, Patrick Fourquet, Stéphane Audebert, Luc Camoin, Stéphane Canaan, Jean-François Cavalier

PLoS ONE 15:e0238178 (2020)10.1371/journal.pone.0238178

2019

2019
Haloarcula sebkhae sp. nov., an extremely halophilic archaeon from Algerian hypersaline environment

Hélène Barreteau, Manon Vandervennet, Laura Guedon, Vanessa Point, Stéphane Canaan, Sylvie Rebuffat, Jean Peduzzi, Alyssa Carré-Mlouka

International Journal of Systematic and Evolutionary Microbiology 69 (2019)10.1099/ijsem.0.003211

2019
Cyclipostins and Cyclophostin Analogues as Multitarget Inhibitors That Impair Growth of Mycobacterium abscessus

Abdeldjalil Madani, Jeremy Ridenour, Benjamin Martin, Rishi Paudel, Anosha Abdul Basir, Vincent Le Moigne, Jean-Louis Herrmann, Stéphane Audebert, Luc Camoin, Laurent Kremer, Christopher Spilling, Stéphane Canaan, Jean-François Cavalier

ACS Infectious Diseases (2019)10.1021/acsinfecdis.9b00172

2019
Worms’ Antimicrobial Peptides

Bruno Renato, Marc Maresca, Stéphane Canaan, Jean-François Cavalier, Kamel Mabrouk, Céline Boidin-Wichlacz, Hamza Olleik, D. Zeppilli, Priscille Brodin, François Massol, Didier Jollivet, Sacha Jung, Aurélie Tasiemski

Marine drugs 17:512 (2019)10.3390/md17090512

2019
Dissecting the membrane lipid binding properties and lipase activity of Mycobacterium tuberculosis LipY domains

Pierre Santucci, Nabil Smichi, Sadia Diomandé, Isabelle Poncin, Vanessa Point, Hélène Gaussier, Jean-François Cavalier, Laurent Kremer, Stéphane Canaan

FEBS Journal (2019)10.1111/febs.14864

2019
Nitrogen deprivation induces triacylglycerol accumulation, drug tolerance and hypervirulence in mycobacteria

Pierre Santucci, Matt Johansen, Vanessa Point, Isabelle Poncin, Albertus Viljoen, Jean-François Cavalier, Laurent Kremer, Stéphane Canaan

Scientific Reports 9 (2019)10.1038/s41598-019-45164-5

2019
Synthesis of Long-Chain β-Lactones and Their Antibacterial Activities against Pathogenic Mycobacteria

Pierre Santucci, Christina Dedaki, Alexandros Athanasoulis, Laura Gallorini, Anaïs Munoz, Stéphane Canaan, Jean-François Cavalier, Victoria Magrioti

ChemMedChem 14:349-358 (2019)10.1002/cmdc.201800720

2019
Methyl arachidonyl fluorophosphonate inhibits Mycobacterium tuberculosis thioesterase TesA and globally affects vancomycin susceptibility

Dong Yang, Guy Vandenbussche, Didier Vertommen, Damien Evrard, Romany Abskharon, Jean-François Cavalier, Gilles Berger, Stéphane Canaan, Mohammad Shahneawz Khan, Sheng Zeng, Alexandre Wohlkonig, Martine Prévost, Patrice Soumillion, Véronique Fontaine

FEBS Letters (2019)10.1002/1873-3468.13555

2018

2018
Biochemical and Structural Characterization of TesA, a Major Thioesterase Required for Outer-Envelope Lipid Biosynthesis in Mycobacterium tuberculosis

Phuong Chi Nguyen, van Son Nguyen, Benjamin Martin, Patrick Fourquet, Luc Camoin, Chistopher Spilling, Jean-François Cavalier, Christian Cambillau, Stéphane Canaan

Journal of Molecular Biology 430:5129-5136 (2018)10.1016/j.jmb.2018.09.017

2018
Oxadiazolone derivatives, new promising multi-target inhibitors against M. tuberculosis

Phuong Chi Nguyen, Vincent Delorme, Anaïs Benarouche, Alexandre Guy, Valérie Landry, Stéphane Audebert, Matthieu Pophillat, Luc Camoin, Céline Crauste, Jean-Marie Galano, Thierry Durand, Priscille Brodin, Stéphane Canaan, Jean-François Cavalier

Bioorganic Chemistry 81:414-424 (2018)10.1016/j.bioorg.2018.08.025

2018
Cyclophostin and Cyclipostins analogues, new promising molecules to treat mycobacterial-related diseases

Phuong Nguyen, Abdeldjalil Madani, Pierre Santucci, Benjamin Martin, Rishi R Paudel, Sandrine Delattre, Jean-Louis Herrmann, Christopher Spilling, Laurent Kremer, Jean-François Cavalier, Stéphane Canaan

International Journal of Antimicrobial Agents (2018)10.1016/j.ijantimicag.2017.12.001

2018
The potent effect of mycolactone on lipid membranes

Milene Nitenberg, Anais Benarouche, Ofelia Maniti, Estelle Marion, Laurent Marsollier, Julie Gean, Erick J. Dufourc, Jean-Francois Cavaller, Stéphane Canaan, Agnes P. Girard-Egrot

PLoS Pathogens 14:e1006814 (2018)10.1371/journal.ppat.1006814

2018
B cells response directed against Cut4 and CFP21 lipolytic enzymes in active and latent tuberculosis infections

Wendy Rénier, Arnaud Bourdin, Pierre-Alain Rubbo, Marianne Peries, Luc Dedieu, Sophie Bendriss, Laurent Kremer, Stéphane Canaan, Dominique Terru, Sylvain Godreuil, Nicolas Nagot, Philippe van de Perre, Edouard Tuaillon

PLoS ONE 13:e0196470 (2018)10.1371/journal.pone.0196470

2018
Delineating the Physiological Roles of the PE and Catalytic Domains of LipY in Lipid Consumption in Mycobacterium-Infected Foamy Macrophages

Pierre Santucci, Sadia Diomandé, Isabelle Poncin, Laetitia Alibaud, Albertus Viljoen, Laurent Kremer, Chantal de Chastellier, Stéphane Canaan

Infection and Immunity 86 (2018)10.1128/IAI.00394-18

2018
LipG a bifunctional phospholipase/thioesterase involved in mycobacterial envelope remodeling

Pierre Santucci, Vanessa Point, Isabelle Poncin, Alexandre Guy, Céline Crauste, Carole Serveau-Avesque, Jean marie Galano, Chistopher d. Spilling, Jean-François Cavalier, Stéphane Canaan

Bioscience Reports 38:BSR20181953 (2018)10.1042/BSR20181953

2018
Lipid Droplets Breakdown: Adipose Triglyceride Lipase Leads the Way

Pierre Santucci, Stéphane Canaan

Current Protein & Peptide Science 19:1131-1133 (2018)10.2174/1389203719666180809143000

2018
Cyclipostins and cyclophostin analogs inhibit the antigen 85C from Mycobacterium tuberculosis both in vitro and in vivo

Albertus Viljoen, Matthias Richard, Phuong Chi Nguyen, Patrick Fourquet, Luc Camoin, Rishi R. Paudal, Giri Gnawali, Christopher Spilling, Jean-François Cavalier, Stéphane Canaan, Mickaël Blaise, Laurent Kremer

Journal of Biological Chemistry 293:2755-2769 (2018)10.1074/jbc.RA117.000760

2017

2017
Mycobacterium canettii Infection of Adipose Tissues

Feriel Bouzid, Fabienne Bregeon, Isabelle Poncin, Pascal Weber, Michel Drancourt, Stéphane Canaan

Frontiers in Cellular and Infection Microbiology 7:189-189 (2017)10.3389/fcimb.2017.00189

2017
Cyclipostins and Cyclophostin analogs as promising compounds in the fight against tuberculosis

Phuong Chi Nguyen, Vincent Delorme, Anaïs Benarouche, Benjamin Martin, Rishi Paudel, Giri Gnawali, Abdeldjalil Madani, Rémy Puppo, Valérie Landry, Laurent Kremer, Priscille Brodin, Christopher Spilling, Jean-François Cavalier, Stéphane Canaan

Scientific Reports 7:11751 (2017)10.1038/s41598-017-11843-4

2017
Scrutiny of Mycobacterium tuberculosis 19 kDa antigen proteoforms provides new insights in the lipoglycoprotein biogenesis paradigm

Julien Parra, Julien Marcoux, Isabelle Poncin, Stéphane Canaan, Jean Herrmann, Jérôme Nigou, Odile Burlet-Schiltz, Michel Rivière

Scientific Reports 7:43682 (2017)10.1038/srep43682

2016

2016
Smooth Tubercle Bacilli: Neglected Opportunistic Tropical Pathogens

Aboubaker Djaltou, Bouzid Feriel, Stéphane Canaan, Michel Drancourt

Frontiers in Public Health 283 (2016)

2016
A phospholipase A 1 antibacterial Type VI secretion effector interacts directly with the C-terminal domain of the VgrG spike protein for delivery

Nicolas Flaugnatti, Thi Thu Hang Le, Stéphane Canaan, Marie-Stéphanie Aschtgen, Vân Nguyên, Stephanie Blangy, Christine Kellenberger, Alain Roussel, Christian Cambillau, E. Cascales, Laure Journet

Molecular Microbiology 99:1099-1118 (2016)10.1111/mmi.13292

2016
Lysosomal Lipases PLRP2 and LPLA2 Process Mycobacterial Multi-acylated Lipids and Generate T Cell Stimulatory Antigens

Martine Gilleron, Marco Lepore, Emilie Layre, Diane Cala-De paepe, Naila Mebarek, James a. Shayman, Stéphane Canaan, Lucia Mori, Frédéric Carrière, Germain Puzo, Gennaro De libero

Cell Chemical Biology 23:1147-1156 (2016)10.1016/j.chembiol.2016.07.021

2016
Pancreatic lipase-related protein 2 and lysosomal phospholipase A2 process mycobacterial multiacylated lipid antigens

Martine Gilleron, Marco Lepore, Emilie Layre, Diane Cala - de Paepe, Naila Mebarek, James Shayman, Stéphane Canaan, Lucia Mori, Frédéric Carrière, Germain Puzo, Gennaro de Libero

Cell Chemical Biology (2016)

2016
Experimental Evolution of Mycobacterium tuberculosis in Human Macrophages Results in Low-Frequency Mutations Not Associated with Selective Advantage

V Guerrini, Subbian Satheshkumar, P. Santucci, Stéphane Canaan, Ml Gennaro, G. Pozzi

PLoS ONE e0167989 (2016)

2016
Experimental Models of Foamy Macrophages and Approaches for Dissecting the Mechanisms of Lipid Accumulation and Consumption during Dormancy and Reactivation of Tuberculosis

Pierre Santucci, Eerie Bouzid, Nabil Smichi, Isabelle Poncin, Laurent Kremer, Chantal de Chastellier, Michel Drancourt, Stéphane Canaan

Frontiers in Cellular and Infection Microbiology 6:122 (2016)10.3389/fcimb.2016.00122

2016
New lipase assay using Pomegranate oil coating in microtiter plates

S Ulker,, C Placidi,, V Point,, Stéphane Canaan, B. Gadenne, Carole Serveau-Avesque, Frédéric Carrière, Jean-François Cavalier

Biochimie 110-118 (2016)

2015

2015
Bacterial phospholipases C as vaccine candidate antigens against cystic fibrosis respiratory pathogens: the Mycobacterium abscessus model

Vincent Le Moigne, Martin Rottman, Céline Goulard, Benoît Barteau, Isabelle Poncin, N Soismier, Stéphane Canaan, Bruno Pitard, Jl Gaillard, Jl Herrmann

Vaccine 33:2118-2124 (2015)10.1016/j.vaccine.2015.03.030

2014

2014
Supported inhibitor for fishing lipases in complex biological media and mass spectrometry identification

Vincent Delormea, Brigitt Raux, Rémy Puppo, Julien Leclaire, Sylvain Marc, Pavan-Kumar Kamarajugadda, Gérard Buono, Frédéric Fotiadu, Stéphane Canaan, Frédéric Carrière

Biochimie 107:124-134 (2014)10.1016/j.biochi.2014.07.015

2013

2013
Mycobacterial lipolytic enzymes: A gold mine for tuberculosis research

Luc Dedieu, C. Serveau-Avesque, L. Kremer, Stéphane Canaan

Biochimie 95:66-73 (2013)10.1016/j.biochi.2012.07.008

2012

2012
MmPPOX Inhibits Mycobacterium tuberculosis Lipolytic Enzymes Belonging to the Hormone-Sensitive Lipase Family and Alters Mycobacterial Growth

Vincent Delorme, Sadia V. Diomandé, Luc Dedieu, Jean-François Cavalier, Fredéric Carrière, Laurent Kremer, Julien Leclaire, Frédéric Fotiadu, Stéphane Canaan

PLoS ONE 7:e46493 (2012)10.1371/journal.pone.0046493

2011

2011
Mycobacterium tuberculosis Lipolytic Enzymes as Potential Biomarkers for the Diagnosis of Active Tuberculosis

Belinda Brust, Mélanie Lecoufle, Edouard Tuaillon, Luc Dedieu, Stéphane Canaan, Viviane Valverde, Laurent Kremer

PLoS ONE 6:e25078 (2011)10.1371/journal.pone.0025078

2011
Identification of putative residues involved in the accessibility of the substrate-binding site of lipoxygenase by site-directed mutagenesis studies.

Cynthia Palmieri-Thiers, Jean-Christophe Alberti, Stéphane Canaan, Virginie Brunini, Claude Gambotti, Félix Tomi, Ernst H Oliw, Liliane Berti, Jacques Maury

Archives of Biochemistry and Biophysics 509:82-9 (2011)10.1016/j.abb.2011.02.008

2010

2010
Two cutinase-like proteins secreted by Mycobacterium tuberculosis show very different lipolytic activities reflecting their physiological function.

Mathieu Schué, Damien Maurin, Rabeb Dhouib, Jean-Claude Bakala N'Goma, Vincent Delorme, Gérard Lambeau, Frédéric Carrière, Stéphane Canaan

FASEB Journal 24:1893-903 (2010)10.1096/fj.09-144766

2009

2009
A lipoxygenase with dual positional specificity is expressed in olives (Olea europaea L.) during ripening

Cynthia Palmieri-Thiers, Stéphane Canaan, Virginie Brunini, Vannina Lorenzi, Félix Tomi, Jean-Luc Desseyn, Ulrike Garscha, Ernst H Oliw, Liliane Berti, Jacques Maury

Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids 1791:339-346 (2009)10.1016/j.bbalip.2009.02.012

2009
A lipoxygenase with dual positional specificity is expressed in olives (Olea europaea L.) during ripening.

Cynthia Palmieri-Thiers, Stéphane Canaan, Virginie Brunini, Vannina Lorenzi, Félix Tomi, Jean-Luc Desseyn, Ulrike Garscha, Ernst H Oliw, Liliane Berti, Jacques Maury

Biochimica et Biophysica Acta - Molecular Cell Research 1791:339-46 (2009)10.1016/j.bbalip.2009.02.012

2008

2008
A genomic search approach to identify esterases in Propionibacterium freudenreichii involved in the formation of flavour in Emmental cheese

Julien Dherbecourt, Hélène Falentin, Stéphane Canaan, Anne Thierry

Microbial Cell Factories 7:1-14 (2008)10.1186/1475-2859-7-16

2007

2007
Gene overexpression and biochemical characterization of the biotechnologically relevant chlorogenic acid hydrolase from Aspergillus niger

Isabelle Benoit, Michèle Asther, Yves Bourne, David Navarro, Stéphane Canaan, Laurence Lesage-Meessen, Marga Herweijer, Pedro M. Coutinho, Marcel Asther, Eric Record

Applied and Environmental Microbiology 73:5624-5632 (2007)10.1128/AEM.00374-07

2007
Characterization of an exported monoglyceride lipase from Mycobacterium tuberculosis possibly involved in the metabolism of host cell membrane lipids

Karen Côtes, Rabeb Dhouib, Isabelle Douchet, Henri Chahinian, Alain de Caro, Frédéric Carrière, Stéphane Canaan

Biochemical Journal 408:417-427 (2007)10.1042/BJ20070745

2006

2006
Neurotoxicity and other pharmacological activities of the snake venom phospholipase A2 OS2: the N-terminal region is more important than enzymatic activity

M. Rouault, L.D. Rash, P. Escoubas, E. Boilard, J. Bollinger, B. Lomonte, T. Maurin, C. Guillaume, Stéphane Canaan, C. Deregnaucourt, J. Schrevel, A. Doglio, J.M. Gutierrez, M. Lazdunski, M. H. Gelb, G. Lambeau

Biochemistry 45:5800-5816 (2006)

2006
LppX is a lipoprotein required for the translocation of phthiocerol dimycocerosates to the surface of Mycobacterium tuberculosis

Gerlind Sulzenbacher, Stéphane Canaan, Yann Bordat, Olivier Neyrolles, Gustavo Stadthagen, Véronique Roig-Zamboni, Jean Rauzier, Damien Maurin, Françoise Laval, Mamadou Daffé, Christian Cambillau, Brigitte Gicquel, Yves Bourne, Mary Jackson

EMBO Journal (2006)10.1038/sj.emboj.7601048

2005

2005
Crystal structure of the conserved hypothetical protein Rv1155 from Mycobacterium tuberculosis

Stéphane Canaan, Gerlind Sulzenbacher, Véronique Roig-Zamboni, Loréna Scappuccini-Calvo, Frédéric Frassinetti, Damien Maurin, Christian Cambillau, Yves Bourne, Christian Griesinger

FEBS Letters (2005)10.1016/j.febslet.2004.11.069

2003

2003
Medium-Scale Structural Genomics: Strategies for Protein Expression and Crystallization

Renaud Vincentelli, Christophe Bignon, Arnaud Gruez, Stéphane Canaan, Gerlind Sulzenbacher, Mariella Tegoni, Rie Campanacci, Christian Cambillau

Accounts of Chemical Research (2003)10.1021/ar010130s

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