Stéphane Canaan
DR2, CNRS
Research group: Lipolysis & Bacterial PathogenicityGroup leader
Phone: +33 (0)4 91 16 40 93
Room: BM311
Publications
2021
2021
Intrabacterial lipid inclusions in mycobacteria: unexpected key players in survival and pathogenesis?
Ivy Mallick, Pierre Santucci, Isabelle Poncin, Vanessa Point, Laurent Kremer, Jean-François Cavalier, Stéphane Canaan
FEMS Microbiology Reviews (2021)10.1093/femsre/fuab029
2021
Transcriptional adaptation of Mycobacterium ulcerans in an original mouse model: New insights into the regulation of mycolactone
Marie Robbe-Saule, Mélanie Foulon, Isabelle Poncin, Lucille Esnault, Hugo Varet, Rachel Legendre, Alban Besnard, Anna Grzegorzewicz, Mary Jackson, Stéphane Canaan, Laurent Marsollier, Estelle Marion
Virulence 12:1438-1451 (2021)10.1080/21505594.2021.1929749
2020
2020
Lipolytic enzymes inhibitors: A new way for antibacterial drugs discovery
Jean-François Cavalier, Christopher Spilling, Thierry Durand, Luc Camoin, Stéphane Canaan
European Journal of Medicinal Chemistry 112908 (2020)10.1016/j.ejmech.2020.112908
2020
Structural basis for loading and inhibition of a bacterial T6 SS phospholipase effector by the VgrG spike
Nicolas Flaugnatti, Chiara Rapisarda, Martial Rey, Solène Beauvois, Viet Anh Nguyen, Stéphane Canaan, Eric Durand, Julia Chamot‐rooke, E. Cascales, Rémi Fronzes, Laure Journet
EMBO Journal 39:e104129 (2020)10.15252/embj.2019104129
2020
A TLR2-Activating Fraction From Mycobacterium abscessus Rough Variant Demonstrates Vaccine and Diagnostic Potential
Vincent Le Moigne, Anne-Laure Roux, Aude Jobart-Malfait, Landry Blanc, Karima Chaoui, Odile Burlet-Schiltz, Jean-Louis Gaillard, Stéphane Canaan, Jérôme Nigou, Jean-Louis Herrmann
Frontiers in Cellular and Infection Microbiology 10 (2020)10.3389/fcimb.2020.00432
2020
Dissecting the antibacterial activity of oxadiazolone-core derivatives against Mycobacterium abscessus
Abdeldjalil Madani, Ivy Mallick, Alexandre Guy, Céline Crauste, Thierry Durand, Patrick Fourquet, Stéphane Audebert, Luc Camoin, Stéphane Canaan, Jean-François Cavalier
PLoS ONE 15:e0238178 (2020)10.1371/journal.pone.0238178
2019
2019
Haloarcula sebkhae sp. nov., an extremely halophilic archaeon from Algerian hypersaline environment
Hélène Barreteau, Manon Vandervennet, Laura Guedon, Vanessa Point, Stéphane Canaan, Sylvie Rebuffat, Jean Peduzzi, Alyssa Carré-Mlouka
International Journal of Systematic and Evolutionary Microbiology 69 (2019)10.1099/ijsem.0.003211
2019
Cyclipostins and Cyclophostin Analogues as Multitarget Inhibitors That Impair Growth of Mycobacterium abscessus
Abdeldjalil Madani, Jeremy Ridenour, Benjamin Martin, Rishi Paudel, Anosha Abdul Basir, Vincent Le Moigne, Jean-Louis Herrmann, Stéphane Audebert, Luc Camoin, Laurent Kremer, Christopher Spilling, Stéphane Canaan, Jean-François Cavalier
ACS Infectious Diseases (2019)10.1021/acsinfecdis.9b00172
2019
Worms’ Antimicrobial Peptides
Bruno Renato, Marc Maresca, Stéphane Canaan, Jean-François Cavalier, Kamel Mabrouk, Céline Boidin-Wichlacz, Hamza Olleik, Daniela Zeppilli, Priscille Brodin, François Massol, Didier Jollivet, Sacha Jung, Aurélie Tasiemski
Marine drugs 17:512 (2019)10.3390/md17090512
2019
Dissecting the membrane lipid binding properties and lipase activity of Mycobacterium tuberculosis LipY domains
Pierre Santucci, Nabil Smichi, Sadia Diomandé, Isabelle Poncin, Vanessa Point, Hélène Gaussier, Jean-François Cavalier, Laurent Kremer, Stéphane Canaan
FEBS Journal (2019)10.1111/febs.14864
2019
Nitrogen deprivation induces triacylglycerol accumulation, drug tolerance and hypervirulence in mycobacteria
Pierre Santucci, Matt Johansen, Vanessa Point, Isabelle Poncin, Albertus Viljoen, Jean-François Cavalier, Laurent Kremer, Stéphane Canaan
Scientific Reports 9 (2019)10.1038/s41598-019-45164-5
2019
Synthesis of Long-Chain β-Lactones and Their Antibacterial Activities against Pathogenic Mycobacteria
Pierre Santucci, Christina Dedaki, Alexandros Athanasoulis, Laura Gallorini, Anaïs Munoz, Stéphane Canaan, Jean-François Cavalier, Victoria Magrioti
ChemMedChem 14:349-358 (2019)10.1002/cmdc.201800720
2019
Methyl arachidonyl fluorophosphonate inhibits Mycobacterium tuberculosis thioesterase TesA and globally affects vancomycin susceptibility
Dong Yang, Guy Vandenbussche, Didier Vertommen, Damien Evrard, Romany Abskharon, Jean-François Cavalier, Gilles Berger, Stéphane Canaan, Mohammad Shahneawz Khan, Sheng Zeng, Alexandre Wohlkonig, Martine Prévost, Patrice Soumillion, Véronique Fontaine
FEBS Letters (2019)10.1002/1873-3468.13555
2018
2018
Biochemical and Structural Characterization of TesA, a Major Thioesterase Required for Outer-Envelope Lipid Biosynthesis in Mycobacterium tuberculosis
Phuong Chi Nguyen, van Son Nguyen, Benjamin Martin, Patrick Fourquet, Luc Camoin, Chistopher Spilling, Jean-François Cavalier, Christian Cambillau, Stéphane Canaan
Journal of Molecular Biology 430:5129-5136 (2018)10.1016/j.jmb.2018.09.017
2018
Oxadiazolone derivatives, new promising multi-target inhibitors against M. tuberculosis
Phuong Chi Nguyen, Vincent Delorme, Anaïs Benarouche, Alexandre Guy, Valérie Landry, Stéphane Audebert, Matthieu Pophillat, Luc Camoin, Céline Crauste, Jean-Marie Galano, Thierry Durand, Priscille Brodin, Stéphane Canaan, Jean-François Cavalier
Bioorganic Chemistry 81:414-424 (2018)10.1016/j.bioorg.2018.08.025
2018
Cyclophostin and Cyclipostins analogues, new promising molecules to treat mycobacterial-related diseases
Phuong Nguyen, Abdeldjalil Madani, Pierre Santucci, Benjamin Martin, Rishi R Paudel, Sandrine Delattre, Jean-Louis Herrmann, Christopher Spilling, Laurent Kremer, Jean-François Cavalier, Stéphane Canaan
International Journal of Antimicrobial Agents (2018)10.1016/j.ijantimicag.2017.12.001
2018
The potent effect of mycolactone on lipid membranes
Milene Nitenberg, Anais Benarouche, Ofelia Maniti, Estelle Marion, Laurent Marsollier, Julie Gean, Erick J. Dufourc, Jean-Francois Cavaller, Stéphane Canaan, Agnes P. Girard-Egrot
PLoS Pathogens 14:e1006814 (2018)10.1371/journal.ppat.1006814
2018
B cells response directed against Cut4 and CFP21 lipolytic enzymes in active and latent tuberculosis infections
Wendy Rénier, Arnaud Bourdin, Pierre-Alain Rubbo, Marianne Peries, Luc Dedieu, Sophie Bendriss, Laurent Kremer, Stéphane Canaan, Dominique Terru, Sylvain Godreuil, Nicolas Nagot, Philippe van de Perre, Edouard Tuaillon
PLoS ONE 13:e0196470 (2018)10.1371/journal.pone.0196470
2018
Delineating the Physiological Roles of the PE and Catalytic Domains of LipY in Lipid Consumption in Mycobacterium-Infected Foamy Macrophages
Pierre Santucci, Sadia Diomandé, Isabelle Poncin, Laetitia Alibaud, Albertus Viljoen, Laurent Kremer, Chantal de Chastellier, Stéphane Canaan
Infection and Immunity 86 (2018)10.1128/IAI.00394-18
2018
LipG a bifunctional phospholipase/thioesterase involved in mycobacterial envelope remodeling
Pierre Santucci, Vanessa Point, Isabelle Poncin, Alexandre Guy, Céline Crauste, Carole Serveau-Avesque, Jean marie Galano, Chistopher d. Spilling, Jean-François Cavalier, Stéphane Canaan
Bioscience Reports 38:BSR20181953 (2018)10.1042/BSR20181953
2018
Lipid Droplets Breakdown: Adipose Triglyceride Lipase Leads the Way
Pierre Santucci, Stéphane Canaan
Current Protein & Peptide Science 19:1131-1133 (2018)10.2174/1389203719666180809143000
2018
Cyclipostins and cyclophostin analogs inhibit the antigen 85C from Mycobacterium tuberculosis both in vitro and in vivo
Albertus Viljoen, Matthias Richard, Phuong Chi Nguyen, Patrick Fourquet, Luc Camoin, Rishi R. Paudal, Giri Gnawali, Christopher Spilling, Jean-François Cavalier, Stéphane Canaan, Mickaël Blaise, Laurent Kremer
Journal of Biological Chemistry 293:2755-2769 (2018)10.1074/jbc.RA117.000760
2017
2017
Mycobacterium canettii Infection of Adipose Tissues
Feriel Bouzid, Fabienne Bregeon, Isabelle Poncin, Pascal Weber, Michel Drancourt, Stéphane Canaan
Frontiers in Cellular and Infection Microbiology 7:189-189 (2017)10.3389/fcimb.2017.00189
2017
Cyclipostins and Cyclophostin analogs as promising compounds in the fight against tuberculosis
Phuong Chi Nguyen, Vincent Delorme, Anaïs Benarouche, Benjamin Martin, Rishi Paudel, Giri Gnawali, Abdeldjalil Madani, Rémy Puppo, Valérie Landry, Laurent Kremer, Priscille Brodin, Christopher Spilling, Jean-François Cavalier, Stéphane Canaan
Scientific Reports 7:11751 (2017)10.1038/s41598-017-11843-4
2017
Scrutiny of Mycobacterium tuberculosis 19 kDa antigen proteoforms provides new insights in the lipoglycoprotein biogenesis paradigm
Julien Parra, Julien Marcoux, Isabelle Poncin, Stéphane Canaan, Jean Herrmann, Jérôme Nigou, Odile Burlet-Schiltz, Michel Rivière
Scientific Reports 7:43682 (2017)10.1038/srep43682
2016
2016
Smooth Tubercle Bacilli: Neglected Opportunistic Tropical Pathogens
Aboubaker Djaltou, Bouzid Feriel, Stéphane Canaan, Michel Drancourt
Frontiers in Public Health 283 (2016)10.3389/fpubh.2015.00283
2016
A phospholipase A 1 antibacterial Type VI secretion effector interacts directly with the C-terminal domain of the VgrG spike protein for delivery
Nicolas Flaugnatti, Thi Thu Hang Le, Stéphane Canaan, Marie-Stéphanie Aschtgen, Vân Nguyên, Stephanie Blangy, Christine Kellenberger, Alain Roussel, Christian Cambillau, E. Cascales, Laure Journet
Molecular Microbiology 99:1099-1118 (2016)10.1111/mmi.13292
2016
Lysosomal Lipases PLRP2 and LPLA2 Process Mycobacterial Multi-acylated Lipids and Generate T Cell Stimulatory Antigens
Martine Gilleron, Marco Lepore, Emilie Layre, Diane Cala-De paepe, Naila Mebarek, James a. Shayman, Stéphane Canaan, Lucia Mori, Frédéric Carrière, Germain Puzo, Gennaro De libero
Cell Chemical Biology 23:1147-1156 (2016)10.1016/j.chembiol.2016.07.021
2016
Pancreatic lipase-related protein 2 and lysosomal phospholipase A2 process mycobacterial multiacylated lipid antigens
Martine Gilleron, Marco Lepore, Emilie Layre, Diane Cala - de Paepe, Naila Mebarek, James Shayman, Stéphane Canaan, Lucia Mori, Frédéric Carrière, Germain Puzo, Gennaro de Libero
Cell Chemical Biology (2016)
2016
Experimental Evolution of Mycobacterium tuberculosis in Human Macrophages Results in Low-Frequency Mutations Not Associated with Selective Advantage
V Guerrini, Subbian Satheshkumar, P. Santucci, Stéphane Canaan, Ml Gennaro, G. Pozzi
PLoS ONE e0167989 (2016)
2016
Experimental Models of Foamy Macrophages and Approaches for Dissecting the Mechanisms of Lipid Accumulation and Consumption during Dormancy and Reactivation of Tuberculosis
Pierre Santucci, Eerie Bouzid, Nabil Smichi, Isabelle Poncin, Laurent Kremer, Chantal de Chastellier, Michel Drancourt, Stéphane Canaan
Frontiers in Cellular and Infection Microbiology 6:122 (2016)10.3389/fcimb.2016.00122
2016
New lipase assay using Pomegranate oil coating in microtiter plates
S Ulker,, C Placidi,, V Point,, Stéphane Canaan, B. Gadenne, Carole Serveau-Avesque, Frédéric Carrière, Jean-François Cavalier
Biochimie 110-118 (2016)
2015
2015
Bacterial phospholipases C as vaccine candidate antigens against cystic fibrosis respiratory pathogens: the Mycobacterium abscessus model
Vincent Le Moigne, Martin Rottman, Céline Goulard, Benoît Barteau, Isabelle Poncin, N Soismier, Stéphane Canaan, Bruno Pitard, Jl Gaillard, Jl Herrmann
Vaccine 33:2118-2124 (2015)10.1016/j.vaccine.2015.03.030
2014
2014
Supported inhibitor for fishing lipases in complex biological media and mass spectrometry identification
Vincent Delormea, Brigitt Raux, Rémy Puppo, Julien Leclaire, Sylvain Marc, Pavan-Kumar Kamarajugadda, Gérard Buono, Frédéric Fotiadu, Stéphane Canaan, Frédéric Carrière
Biochimie 107:124-134 (2014)10.1016/j.biochi.2014.07.015
2013
2013
Identification of Residues Involved in Substrate Specificity and Cytotoxicity of Two Closely Related Cutinases from Mycobacterium tuberculosis
Luc Dedieu, Carole Serveau-Avesque, Stéphane Canaan
PLoS ONE 8:e66913 (2013)10.1371/journal.pone.0066913
2013
Mycobacterial lipolytic enzymes: A gold mine for tuberculosis research
Luc Dedieu, C. Serveau-Avesque, L. Kremer, Stéphane Canaan
Biochimie 95:66-73 (2013)10.1016/j.biochi.2012.07.008
2012
2012
MmPPOX Inhibits Mycobacterium tuberculosis Lipolytic Enzymes Belonging to the Hormone-Sensitive Lipase Family and Alters Mycobacterial Growth
Vincent Delorme, Sadia V. Diomandé, Luc Dedieu, Jean-François Cavalier, Fredéric Carrière, Laurent Kremer, Julien Leclaire, Frédéric Fotiadu, Stéphane Canaan
PLoS ONE 7:e46493 (2012)10.1371/journal.pone.0046493
2011
2011
Mycobacterium tuberculosis Lipolytic Enzymes as Potential Biomarkers for the Diagnosis of Active Tuberculosis
Belinda Brust, Mélanie Lecoufle, Edouard Tuaillon, Luc Dedieu, Stéphane Canaan, Viviane Valverde, Laurent Kremer
PLoS ONE 6:e25078 (2011)10.1371/journal.pone.0025078
2011
Identification of putative residues involved in the accessibility of the substrate-binding site of lipoxygenase by site-directed mutagenesis studies.
Cynthia Palmieri-Thiers, Jean-Christophe Alberti, Stéphane Canaan, Virginie Brunini, Claude Gambotti, Félix Tomi, Ernst H Oliw, Liliane Berti, Jacques Maury
Archives of Biochemistry and Biophysics 509:82-9 (2011)10.1016/j.abb.2011.02.008
2010
2010
Two cutinase-like proteins secreted by Mycobacterium tuberculosis show very different lipolytic activities reflecting their physiological function.
Mathieu Schué, Damien Maurin, Rabeb Dhouib, Jean-Claude Bakala N'Goma, Vincent Delorme, Gérard Lambeau, Frédéric Carrière, Stéphane Canaan
FASEB Journal 24:1893-903 (2010)10.1096/fj.09-144766
2009
2009
A lipoxygenase with dual positional specificity is expressed in olives (Olea europaea L.) during ripening
Cynthia Palmieri-Thiers, Stéphane Canaan, Virginie Brunini, Vannina Lorenzi, Félix Tomi, Jean-Luc Desseyn, Ulrike Garscha, Ernst H Oliw, Liliane Berti, Jacques Maury
Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids 1791:339-346 (2009)10.1016/j.bbalip.2009.02.012
2009
A lipoxygenase with dual positional specificity is expressed in olives (Olea europaea L.) during ripening.
Cynthia Palmieri-Thiers, Stéphane Canaan, Virginie Brunini, Vannina Lorenzi, Félix Tomi, Jean-Luc Desseyn, Ulrike Garscha, Ernst H Oliw, Liliane Berti, Jacques Maury
Biochimica et Biophysica Acta - Molecular Cell Research 1791:339-46 (2009)10.1016/j.bbalip.2009.02.012
2008
2008
A genomic search approach to identify esterases in Propionibacterium freudenreichii involved in the formation of flavour in Emmental cheese
Julien Dherbecourt, Hélène Falentin, Stéphane Canaan, Anne Thierry
Microbial Cell Factories 7:1-14 (2008)10.1186/1475-2859-7-16
2007
2007
Gene overexpression and biochemical characterization of the biotechnologically relevant chlorogenic acid hydrolase from Aspergillus niger
Isabelle Benoit, Michèle Asther, Yves Bourne, David Navarro, Stéphane Canaan, Laurence Lesage-Meessen, Marga Herweijer, Pedro Coutinho, Marcel Asther, Eric Record
Applied and Environmental Microbiology 73:5624-5632 (2007)10.1128/AEM.00374-07
2007
Characterization of an exported monoglyceride lipase from Mycobacterium tuberculosis possibly involved in the metabolism of host cell membrane lipids
Karen Côtes, Rabeb Dhouib, Isabelle Douchet, Henri Chahinian, Alain de Caro, Frédéric Carrière, Stéphane Canaan
Biochemical Journal 408:417-427 (2007)10.1042/BJ20070745
2006
2006
Neurotoxicity and other pharmacological activities of the snake venom phospholipase A2 OS2: the N-terminal region is more important than enzymatic activity
M. Rouault, L.D. Rash, P. Escoubas, E. Boilard, J. Bollinger, B. Lomonte, T. Maurin, C. Guillaume, Stéphane Canaan, C. Deregnaucourt, J. Schrevel, A. Doglio, J.M. Gutierrez, M. Lazdunski, M. H. Gelb, G. Lambeau
Biochemistry 45:5800-5816 (2006)
2006
LppX is a lipoprotein required for the translocation of phthiocerol dimycocerosates to the surface of Mycobacterium tuberculosis
Gerlind Sulzenbacher, Stéphane Canaan, Yann Bordat, Olivier Neyrolles, Gustavo Stadthagen, Véronique Roig-Zamboni, Jean Rauzier, Damien Maurin, Françoise Laval, Mamadou Daffé, Christian Cambillau, Brigitte Gicquel, Yves Bourne, Mary Jackson
EMBO Journal (2006)10.1038/sj.emboj.7601048
2005
2005
Crystal structure of the conserved hypothetical protein Rv1155 from Mycobacterium tuberculosis
Stéphane Canaan, Gerlind Sulzenbacher, Véronique Roig-Zamboni, Loréna Scappuccini-Calvo, Frédéric Frassinetti, Damien Maurin, Christian Cambillau, Yves Bourne, Christian Griesinger
FEBS Letters (2005)10.1016/j.febslet.2004.11.069
2003
2003
Medium-Scale Structural Genomics: Strategies for Protein Expression and Crystallization
Renaud Vincentelli, Christophe Bignon, Arnaud Gruez, Stéphane Canaan, Gerlind Sulzenbacher, Mariella Tegoni, Rie Campanacci, Christian Cambillau
Accounts of Chemical Research (2003)10.1021/ar010130s