Cell contacts with the microenvironment orchestrate the development of organisms, homeostasis, and the functions of individual cells. When cells do not interact correctly or decode external molecular messages incorrectly, cellular responses are disturbed, and diseases can develop.
Therefore, understanding the structural basis of protein assemblies involved in the sophisticated mechanisms of action developed by cells to interact/communicate with their microenvironment is of main importance.
Our research is focused on the study of these membrane-associated protein complexes. The primary focus of our research is on how human Galectins interactions are regulated at the cell surface during cell-cell interactions.
This study involves protein-protein as well as protein-carbohydrates interactions. We also seek to understand the mechanisms regulating bacterial cell motility from structural studies of focal adhesion protein complexes in M. xanthus.
To pursue our goals, we use Nuclear Magnetic resonance (NMR) spectroscopy combined with biochemical and biophysical techniques in an integrated structural biology approach. NMR can provide an atomic-level view of the structure and dynamics of macromolecular systems in solution. A particular strength of NMR is its ability to reveal the structural details of specific interactions between biological macromolecules, even in the case of transient complexes between proteins and their targets in solution.
Overall, our aim is to decipher at atomic level the assembly of multi-protein complexes, determine their structures, gain insight into their activities and regulation, and identify roles for proteins or domains of unknown function.
Gauthier Dangla Pélissier, Nicolas Roux, Victoria Schmidt, Gaël Chambonnier, Moly Ba, Corinne Sebban-Kreuzer, Sophie de Bentzmann, Caroline Giraud, Christophe Bordi
Nucleic Acids Research 49:10956-10974 (2021)10.1093/nar/gkab827
Lucía Sanjurjo, Iris A. Schulkens, Pauline Touarin, Roy Heusschen, Ed Aanhane, Kitty C.M. Castricum, Tanja D. De Gruijl, Ulf J. Nilsson, Hakon Leffler, Arjan W. Griffioen, Latifa Elantak, Rory R. Koenen, Victor L.J.L. Thijssen
Communications Biology (2021)
Clarisse Roblin, Steve Chiumento, Olivier Bornet, Matthieu Nouailler, Christina S. Müller, Katy Jeannot, Christian Basset, Sylvie Kieffer-Jaquinod, Yohann Couté, Stéphane Torelli, Laurent Le pape, Volker Schünemann, Hamza Olleik, Bruno de la Villeon, Philippe Sockeel, Eric Di Pasquale, Cendrine Nicoletti, Nicolas Vidal, Leonora Poljak, Olga Iranzo, Thierry Giardina, Michel Fons, Estelle Devillard, Patrice Polard, Marc Maresca, Josette Perrier, Mohamed Atta, Francoise Guerlesquin, Mickaël Lafond, Victor Duarte
Proceedings of the National Academy of Sciences of the United States of America 117:19168-19177 (2020)10.1073/pnas.2004045117
Mickaël Canestrari, Bastien Serrano, Julia Bartoli, Valerie Prima, Olivier Bornet, Rémy Puppo, Emmanuelle Bouveret, Francoise Guerlesquin, Julie Viala
FEBS Letters (2019)10.1002/1873-3468.13593
Chiara Rapisarda, Yassine Cherrak, Romain Kooger, Victoria Schmidt, Riccardo Pellarin, Laureen Logger, E. Cascales, Martin Pilhofer, Eric Durand, Rémi Fronzes
EMBO Journal 38 (2019)10.15252/embj.2018100886
Victoria Schmidt, Marlon Sidore, Cherine Bechara, Jean-Pierre Duneau, James Sturgis
Biochimica et Biophysica Acta:Biomembranes 1861:431-440 (2019)10.1016/j.bbamem.2018.10.017
Victoria Schmidt, James Sturgis
Bio-protocol 8 (2018)10.21769/BioProtoc.2969
Victoria Schmidt, James Sturgis
Biochimica et Biophysica Acta:Biomembranes 1860:777-783 (2018)10.1016/j.bbamem.2017.12.012
Laetitia Houot, Romain Navarro, Matthieu Nouailler, Denis Duché, Francoise Guerlesquin, Roland Lloubès
Journal of Biological Chemistry 292:13584-13598 (2017)10.1074/jbc.M117.786061
Victoria Schmidt, James Sturgis
ACS Omega 2:3017-3027 (2017)10.1021/acsomega.7b00261
Yinghui Wang, Louise Pinet, Nadine Assrir, Latifa Elantak, Francoise Guerlesquin, Ali Badache, Ewen Lescop, Carine van Heijenoort
Biomolecular NMR Assignments 12:23-26 (2017)10.1007/s12104-017-9773-4
Romain Navarro, Olivier Bornet, Laetitia Houot, Roland Lloubes, Francoise Guerlesquin, Matthieu Nouailler
Biomol NMR Assign (2016)10.1007/s12104-016-9690-y
Jeremy Bonzi, Olivier Bornet, Stéphane Betzi, Brian Kasper, Lara Mahal, Stéphane Mancini, Claudine Schiff, Corinne Sebban-Kreuzer, Francoise Guerlesquin, Latifa Elantak
Nature Communications 6:6194 (2015)10.1038/ncomms7194
Stephane J C Mancini, Latifa El Antak, Annie Boned, Marion Espeli, Francoise Guerlesquin, Claudine Schiff
Methods in Molecular Biology 1207:169-84 (2015)10.1007/978-1-4939-1396-1_11
Victoria Schmidt, Pierre Hubert, James Sturgis
Eur. Biophys. J. 44:S217 (2015)
Olivier Bornet, Matthieu Nouailler, Michaël Feracci, Corinne Sebban-Kreuzer, Deborah Byrne, Hubert Halimi, Xavier Morelli, Ali Badache, Francoise Guerlesquin
FEBS Letters 588:2031-6 (2014)10.1016/j.febslet.2014.04.029
Edwige B Garcin, Olivier Bornet, Latifa El Antak, Matthieu Nouailler, Francoise Guerlesquin, Corinne Sebban-Kreuzer
Biomol NMR Assign 8:247-50 (2014)10.1007/s12104-013-9493-3
H Halimi, A. Rigato, D. Byrne, G. Ferracci, Corinne Sebban-Kreuzer, Latifa Elantak, Francoise Guerlesquin
PLoS ONE 9 (2014)
Victoria Schmidt, Pierre Hubert, Valerie Prima, James Sturgis
Biophysical Journal 108:499a-500a (2014)
Latifa Elantak, Marion Espeli, Annie Boned, Olivier Bornet, Jeremy Bonzi, Laurent Gauthier, Mikael Feracci, Philippe Roche, Francoise Guerlesquin, Claudine Schiff
Journal of Biological Chemistry 287:44703-44713 (2012)10.1074/jbc.m112.395152
Tania Puvirajesinghe, Latifa Elantak, Sabrina Lignon, Nathalie Franche, Marianne Ilbert, Mireille Ansaldi
Journal of Biological Chemistry 287:14169-14177 (2012)10.1074/jbc.m111.331462
Latifa Elantak, Susan Wagner, Anna Herrmannová, Martina Karásková, Edit Rutkai, Peter Lukavsky, Leoš Valášek
Journal of Molecular Biology 396:1097-1116 (2010)10.1016/j.jmb.2009.12.047
Latifa Elantak, Andreas Tzakos, Nicolas Locker, Peter Lukavsky
Journal of Biological Chemistry 282:8165-8174 (2007)10.1074/jbc.m610860200