Understanding the structural basis of protein assemblies that are functionally important for various aspects of cell contacts with its microenvironment.

Cell contacts with the microenvironment orchestrate the development of organisms, homeostasis, and the functions of individual cells. When cells do not interact correctly or decode external molecular messages incorrectly, cellular responses are disturbed, and diseases can develop.

Therefore, understanding the structural basis of protein assemblies involved in the sophisticated mechanisms of action developed by cells to interact/communicate with their microenvironment is of main importance.

Our research is focused on the study of these membrane-associated protein complexes. The primary focus of our research is on how human Galectins interactions are regulated at the cell surface during cell-cell interactions.

This study involves protein-protein as well as protein-carbohydrates interactions. We also seek to understand the mechanisms regulating bacterial cell motility from structural studies of focal adhesion protein complexes in M. xanthus.

Exemple of structural investigations performed in the group on cell-cell interactions involving Galectin-1 during B cell development in the bone marrow
(Elantak et al. 2012, Bonzi et al. 2015).

To pursue our goals, we use Nuclear Magnetic resonance (NMR) spectroscopy combined with biochemical and biophysical techniques in an integrated structural biology approach. NMR can provide an atomic-level view of the structure and dynamics of macromolecular systems in solution. A particular strength of NMR is its ability to reveal the structural details of specific interactions between biological macromolecules, even in the case of transient complexes between proteins and their targets in solution.

Overall, our aim is to decipher at atomic level the assembly of multi-protein complexes, determine their structures, gain insight into their activities and regulation, and identify roles for proteins or domains of unknown function.

Publications

2020
The unusual structure of Ruminococcin C1 antimicrobial peptide confers clinical properties

Clarisse Roblin, Steve Chiumento, Olivier Bornet, Matthieu Nouailler, Christina S. Müller, Katy Jeannot, Christian Basset, Sylvie Kieffer-Jaquinod, Yohann Couté, Stéphane Torelli, Laurent Le pape, Volker Schünemann, Hamza Olleik, Bruno de la Villeon, Philippe Sockeel, Eric Di Pasquale, Cendrine Nicoletti, Nicolas Vidal, Leonora Poljak, Olga Iranzo, Thierry Giardina, Michel Fons, Estelle Devillard, Patrice Polard, Marc Maresca, Josette Perrier, Mohamed Atta, Francoise Guerlesquin, Mickaël Lafond, Victor Duarte

Proceedings of the National Academy of Sciences of the United States of America 117:19168-19177 (2020)10.1073/pnas.2004045117

2019
Deciphering the specific interaction between the acyl carrier protein IacP and the T3SS‐major hydrophobic translocator SipB from Salmonella

Mickaël Canestrari, Bastien Serrano, Julia Bartoli, Valerie Prima, Olivier Bornet, Rémy Puppo, Emmanuelle Bouveret, Francoise Guerlesquin, Julie Viala

FEBS Letters (2019)10.1002/1873-3468.13593

2019
In situ and high-resolution Cryo-EM structure of the Type VI secretion membrane complex

Chiara Rapisarda, Yassine Cherrak, Romain Kooger, Victoria Schmidt, Riccardo Pellarin, Laureen Logger, E. Cascales, Martin Pilhofer, Eric Durand, Rémi Fronzes

EMBO Journal 38 (2019)10.15252/embj.2018100886

2019
The lipid environment of Escherichia coli Aquaporin Z

Victoria Schmidt, Marlon Sidore, Chérine Bechara, Jean-Pierre Duneau, James Sturgis

Biochimica et Biophysica Acta:Biomembranes 1861:431-440 (2019)10.1016/j.bbamem.2018.10.017

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2018
Modifying styrene-maleic acid co-polymer for studying lipid nanodiscs

Victoria Schmidt, James Sturgis

Biochimica et Biophysica Acta:Biomembranes 1860:777-783 (2018)10.1016/j.bbamem.2017.12.012

2017
Electrostatic interactions between the CTX phage minor coat protein and the bacterial host receptor TolA drive the pathogenic conversion of Vibrio cholerae

Laetitia Houot, Romain Navarro, Matthieu Nouailler, Denis Duché, Francoise Guerlesquin, Roland Lloubès

Journal of Biological Chemistry 292:13584-13598 (2017)10.1074/jbc.M117.786061

2017
1H, 13C and 15N assignments of the C-terminal intrinsically disordered cytosolic fragment of the receptor tyrosine kinase ErbB2

Yinghui Wang, Louise Pinet, Nadine Assrir, Latifa Elantak, Francoise Guerlesquin, Ali Badache, Ewen Lescop, Carine van Heijenoort

Biomolecular NMR Assignments 12:23-26 (2017)10.1007/s12104-017-9773-4

2016
(1)H, (15)N and (13)C resonance assignments of the C-terminal domain of Vibrio cholerae TolA protein.

Romain Navarro, Olivier Bornet, Laetitia Houot, Roland Lloubes, Francoise Guerlesquin, Matthieu Nouailler

Biomol NMR Assign (2016)10.1007/s12104-016-9690-y

2015
Pre-B cell receptor binding to galectin-1 modifies galectin-1/carbohydrate affinity to modulate specific galectin-1/glycan lattice interactions

Jeremy Bonzi, Olivier Bornet, Stéphane Betzi, Brian Kasper, Lara Mahal, Stéphane Mancini, Claudine Schiff, Corinne Sebban-Kreuzer, Francoise Guerlesquin, Latifa Elantak

Nature Communications 6:6194 (2015)10.1038/ncomms7194

2015
Examination of galectin-induced lattice formation on early B-cell development.

Stephane J C Mancini, Latifa El Antak, Annie Boned, Marion Espeli, Francoise Guerlesquin, Claudine Schiff

Methods in Molecular Biology 1207:169-84 (2015)10.1007/978-1-4939-1396-1_11

2015
The E.coli Aquaporin Z interface : a puzzle?

Victoria Schmidt, Pierre Hubert, James Sturgis

Eur. Biophys. J. 44:S217 (2015)

2014
Identification of a Src kinase SH3 binding site in the C-terminal domain of the human ErbB2 receptor tyrosine kinase.

Olivier Bornet, Matthieu Nouailler, Michaël Feracci, Corinne Sebban-Kreuzer, Deborah Byrne, Hubert Halimi, Xavier Morelli, Ali Badache, Francoise Guerlesquin

FEBS Letters 588:2031-6 (2014)10.1016/j.febslet.2014.04.029

2014
1H, 13C and 15N backbone and side-chain chemical shift assignments for reduced unusual thioredoxin Patrx2 of Pseudomonas aeruginosa.

Edwige B Garcin, Olivier Bornet, Latifa El Antak, Matthieu Nouailler, Francoise Guerlesquin, Corinne Sebban-Kreuzer

Biomol NMR Assign 8:247-50 (2014)10.1007/s12104-013-9493-3

2014
Glycan Dependence of Galectin-3 Self-Association Properties

H Halimi, A. Rigato, D. Byrne, G. Ferracci, Corinne Sebban-Kreuzer, Latifa Elantak, Francoise Guerlesquin

PLoS ONE 9 (2014)

2014
Structural and functional characterization of the Clostridium perfringens N-acetylmannosamine-6-phosphate 2-epimerase essential for the sialic acid salvage pathway.

Marie-Cécile Pélissier, Corinne Sebban-Kreuzer, Francoise Guerlesquin, James A Brannigan, Yves Bourne, Florence Vincent

Journal of Biological Chemistry 289:35215-24 (2014)10.1074/jbc.M114.604272

2014
Destabilizing Aquaporin Z Assembly: Effects on Structure, Function and Dynamics

Victoria Schmidt, Pierre Hubert, Valerie Prima, James Sturgis

Biophysical Journal 108:499a-500a (2014)

2012
Structural Basis for Galectin-1-dependent Pre-B Cell Receptor (Pre-BCR) Activation

Latifa Elantak, Marion Espeli, Annie Boned, Olivier Bornet, Jeremy Bonzi, Laurent Gauthier, Mikael Feracci, Philippe Roche, Francoise Guerlesquin, Claudine Schiff

Journal of Biological Chemistry 287:44703-44713 (2012)10.1074/jbc.m112.395152

2012
Structural and Mechanistic Insights into Unusual Thiol Disulfide Oxidoreductase

Edwige Garcin, Olivier Bornet, Latifa Elantak, Nicolas Vita, Laetitia Pieulle, Francoise Guerlesquin, Corinne Sebban-Kreuzer

Journal of Biological Chemistry 287:1688-1697 (2012)10.1074/jbc.m111.288316

2012
DnaJ (Hsp40 Protein) Binding to Folded Substrate Impacts KplE1 Prophage Excision Efficiency

Tania Puvirajesinghe, Latifa Elantak, Sabrina Lignon, Nathalie Franche, Marianne Ilbert, Mireille Ansaldi

Journal of Biological Chemistry 287:14169-14177 (2012)10.1074/jbc.M111.331462

2010
The Indispensable N-Terminal Half of eIF3j/HCR1 Cooperates with its Structurally Conserved Binding Partner eIF3b/PRT1-RRM and with eIF1A in Stringent AUG Selection

Latifa Elantak, Susan Wagner, Anna Herrmannová, Martina Karásková, Edit Rutkai, Peter Lukavsky, Leoš Valášek

Journal of Molecular Biology 396:1097-1116 (2010)10.1016/j.jmb.2009.12.047

2007
Structure of eIF3b RNA Recognition Motif and Its Interaction with eIF3j

Latifa Elantak, Andreas Tzakos, Nicolas Locker, Peter Lukavsky

Journal of Biological Chemistry 282:8165-8174 (2007)10.1074/jbc.m610860200

2005
Structural and Genetic Analyses Reveal a Key Role in Prophage Excision for the TorI Response Regulator Inhibitor

Latifa Elantak, Mireille Ansaldi, Francoise Guerlesquin, Vincent Méjean, Xavier Morelli

Journal of Biological Chemistry 280:36802-36808 (2005)10.1074/jbc.m507409200

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Collaborations

  • National
    • Tâm Mignot, LCB UMR7283, Marseille
    • Romain Mercier, LCB UMR7283, Marseille
    • Stephane Mancini, Rennes
    • Guido Pintacuda, Lyon
  • International
    • Victor Thijssen, Amsterdam
    • Daniel Abankwa, Luxembourg
    • Lincoln Scott, San Diego

Funding

© 2021 LISM - Laboratoire d'Ingénierie des Systèmes Macromoléculaires - CNRS UMR7255

31 Chemin Joseph Aiguier, 13402 Marseille cedex 20, France