The “Sensing environment & community lifestyle” team is studying the regulatory pathways
that control biofilm formation in P. aeruginosa as well as the processes of evolution and
adaptation that this bacterium undergoes in this lifestyle.

90% of the microbiological activity in an ecosystem (aquatic environment, soil, human body, etc.) comes for the most part from sedentary flora adhering to surfaces in a structure called biofilm, which is an aggregate of micro-organisms (mostly bacteria), fixed to a surface, and stuck in a self-produced gangue of exopolymers.

The presence of biofilms is increasingly identified as the recurring source of both public health and industrial problems, which are often serious and sometimes dramatic. Pseudomonas aeruginosa is a gram-negative bacterium, an opportunistic human pathogen with a very high adaptive capacity since it can colonise many ecological niches.

Practically harmless in healthy people, this Gram-negative environmental bacillus can become a deadly threat to immunodeficient individuals, intubated-ventilated patients in intensive care units or those suffering from chronic diseases such as cystic fibrosis.

Biofilm formation monitored in flow-cell after 4 Days of growth and treats exposed to tobramycin. Dead cells were labeled red.

During chromic infections, P. aeruginosa adopts a biofilm lifestyle that allows it to evade the immune system, limits the penetration of any antibiotic treatment and promotes the emergence of specific resistances.

The Sensing environment & community lifestyle team studies, using genomic, fluorescent microscopy and structural biology the regulatory pathways that trigger the formation of the biofilm and the evolutionary and adaptive processes that P. aeruginosa undergoes in its lifestyle. Our aim is to propose alternative therapeutic strategies for the treatment of P. aeruginosa infections.

Members

Past Members / Alumni

  • Gaël Chambonnier : PhD student
  • Firas Fadel : Post-Doc
  • Coralie Marchand : Master 2 MBVB
  • Gianlucca Nicastro : Post-Doc
  • David Redelberger : Technical staff
  • Sarah Bigot : Researcher
  • Nicolas Roux : PhD student
  • Sophie de Bentzmann : INSERM Researcher
  • Lorène Roux : PhD student
  • Jennifer Spagnolo : PhD student
  • David Redelberger : Technical staff
  • Christophe Bernard : PhD student
  • Caroline Giraud : PhD student
  • Friederike Ewald : Master 2 BBSG
  • Laetitia Houot : Post Doc
  • Segolène Ruer : PhD student

Publications

2020
The phosphorylated regulator of chemotaxis is crucial throughout biofilm biogenesis in Shewanella oneidensis

Anne Boyeldieu, Amine Ali Chaouche, Moly Ba, Flora Ambre Honoré, Vincent Méjean, Cécile Jourlin-Castelli

npj Biofilms and Microbiomes 6 (2020)10.1038/s41522-020-00165-5

2019
Connected partner-switches control the life style of Pseudomonas aeruginosa through RpoS regulation

Sophie Bouillet, Moly Ba, Laetitia Houot, Chantal Iobbi-Nivol, Christophe Bordi

Scientific Reports 9 (2019)10.1038/s41598-019-42653-5

2018
Constitutive activation of MexT by amino acid substitutions results in MexEF-OprN overproduction in clinical isolates of Pseudomonas aeruginosa

Paulo Juarez, Isabelle Broutin, Christophe Bordi, Patrick Plesiat, Catherine Llanes

Antimicrobial Agents and Chemotherapy (2018)10.1128/AAC.02445-17

2017
NMR assignments of the GacS histidine-kinase periplasmic detection domain from Pseudomonas aeruginosa PAO1

Ahmad Ali-Ahmad, Olivier Bornet, Firas Fadel, Yves Bourne, Florence Vincent, Christophe Bordi, Françoise Guerlesquin, Corinne Sebban-Kreuzer

Biomolecular NMR Assignments 11:25-28 (2017)10.1007/s12104-016-9714-7

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2017
Structural and functional insights into the periplasmic detector domain of the GacS histidine kinase controlling biofilm formation in Pseudomonas aeruginosa

Ahmad Ali-Ahmad, Firas Fadel, Corinne Sebban-Kreuzer, Moly Ba, Gauthier Pélissier, Olivier Bornet, Françoise Guerlesquin, Yves Bourne, Christophe Bordi, Florence Vincent

Scientific Reports 7:11262 (2017)10.1038/s41598-017-11361-3

2017
Cyclic-di-GMP regulates lipopolysaccharide modification and contributes to Pseudomonas aeruginosa immune evasion Europe PMC Funders Group

Ronan Mccarthy, Maria Mazon-Moya, Joana Moscoso, Youai Hao, Joseph Lam, Christophe Bordi, Serge Mostowy, Alain Filloux

Nature Microbiology 2:17027 (2017)10.1038/nmicrobiol.2017.27

2016
The Hybrid Histidine Kinase LadS Forms a Multicomponent Signal Transduction System with the GacS/GacA Two-Component System in Pseudomonas aeruginosa

Gaël Chambonnier, Lorène Roux, David Redelberger, Firas Fadel, Alain Filloux, Melissa Sivaneson, Sophie Bentzmann, Christophe Bordi

PLoS Genetics 12:e1006032 (2016)10.1371/journal.pgen.1006032

2016
M-CSF improves protection against bacterial and fungal infections after hematopoietic stem/progenitor cell transplantation

Prashanth Kandalla, Sandrine Sarrazin, Kaaweh Molawi, Carole Berruyer, David Redelberger, Anne Favel, Christophe Bordi, Sophie de Bentzmann, Michael Sieweke

Journal of Experimental Medicine 213:2269-2279 (2016)10.1084/jem.20151975

2015
Pre-B cell receptor binding to galectin-1 modifies galectin-1/carbohydrate affinity to modulate specific galectin-1/glycan lattice interactions

Jeremy Bonzi, Olivier Bornet, Stéphane Betzi, Brian Kasper, Lara Mahal, Stéphane Mancini, Claudine Schiff, Corinne Sebban-Kreuzer, Francoise Guerlesquin, Latifa Elantak

Nature Communications 6:6194 (2015)10.1038/ncomms7194

2014
Construction of Pseudomonas aeruginosa two-hybrid libraries for high-throughput assays.

Sophie Bentzmann, Christophe Bordi

Methods in Molecular Biology 1149:225-41 (2014)10.1007/978-1-4939-0473-0_19

2014
Construction of a Pseudomonas aeruginosa genomic DNA library.

Christophe Bordi

Methods in Molecular Biology 1149:555-63 (2014)10.1007/978-1-4939-0473-0_42

2014
Identification of a Src kinase SH3 binding site in the C-terminal domain of the human ErbB2 receptor tyrosine kinase.

Olivier Bornet, Matthieu Nouailler, Michaël Feracci, Corinne Sebban-Kreuzer, Deborah Byrne, Hubert Halimi, Xavier Morelli, Ali Badache, Francoise Guerlesquin

FEBS Letters 588:2031-6 (2014)10.1016/j.febslet.2014.04.029

2014
1H, 13C and 15N backbone and side-chain chemical shift assignments for reduced unusual thioredoxin Patrx2 of Pseudomonas aeruginosa.

Edwige B Garcin, Olivier Bornet, Latifa El Antak, Matthieu Nouailler, Francoise Guerlesquin, Corinne Sebban-Kreuzer

Biomol NMR Assign 8:247-50 (2014)10.1007/s12104-013-9493-3

2014
Glycan Dependence of Galectin-3 Self-Association Properties

H Halimi, A. Rigato, D. Byrne, G. Ferracci, Corinne Sebban-Kreuzer, Latifa Elantak, Francoise Guerlesquin

PLoS ONE 9 (2014)

2014
Pseudomonas aeruginosa Genome Evolution in Patients and under the Hospital Environment.

Céline Lucchetti-Miganeh, David Redelberger, Gaël Chambonnier, François Rechenmann, Sylvie Elsen, Christophe Bordi, Katy Jeannot, Ina Attrée, Patrick Plésiat, Sophie Bentzmann

Pathogens 3:309-40 (2014)10.3390/pathogens3020309

2014
Structural and Functional Characterization of the Clostridium perfringens N-Acetylmannosamine-6-phosphate 2-Epimerase Essential for the Sialic Acid Salvage Pathway

Marie-Cécile Pélissier, Corinne Sebban-Kreuzer, Françoise Guerlesquin, James Brannigan, Yves Bourne, Florence Vincent

Journal of Biological Chemistry 289:35215-35224 (2014)10.1074/jbc.m114.604272

2014
Structural and functional characterization of the Clostridium perfringens N-acetylmannosamine-6-phosphate 2-epimerase essential for the sialic acid salvage pathway.

Marie-Cécile Pélissier, Corinne Sebban-Kreuzer, Francoise Guerlesquin, James A Brannigan, Yves Bourne, Florence Vincent

Journal of Biological Chemistry 289:35215-24 (2014)10.1074/jbc.M114.604272

2014
A gacS deletion in Pseudomonas aeruginosa cystic fibrosis isolate CHA shapes its virulence

Khady Mayebine Sall, Maria Guillermina Casabona, Christophe Bordi, Philippe Huber, Sophie Bentzmann, Ina Attrée, Sylvie Elsen

PLoS ONE 9:e95936 (2014)10.1371/journal.pone.0095936

2012
Unique biofilm signature, drug susceptibility and decreased virulence in Drosophila through the Pseudomonas aeruginosa two-component system PprAB.

Sophie de Bentzmann, Caroline Giraud, Christophe Bernard, Virginie Calderon, Friederike Ewald, Patrick Plésiat, Cathy Nguyen, Didier Grunwald, Ina Attree, Katy Jeannot, Marie-Odile Fauvarque, Christophe Bordi

PLoS Pathogens 8:e1003052 (2012)10.1371/journal.ppat.1003052

2012
Structural and mechanistic insights into unusual thiol disulfide oxidoreductase.

Edwige B Garcin, Olivier Bornet, Latifa El Antak, Nicolas Vita, Laetitia Pieulle, Françoise Guerlesquin, Corinne Sebban-Kreuzer

Journal of Biological Chemistry 287:1688-97 (2012)10.1074/jbc.M111.288316

2012
Structural and Mechanistic Insights into Unusual Thiol Disulfide Oxidoreductase

Edwige Garcin, Olivier Bornet, Latifa Elantak, Nicolas Vita, Laetitia Pieulle, Francoise Guerlesquin, Corinne Sebban-Kreuzer

Journal of Biological Chemistry 287:1688-1697 (2012)10.1074/jbc.m111.288316

2012
A bacterial two-hybrid genome fragment library for deciphering regulatory networks of the opportunistic pathogen Pseudomonas aeruginosa.

Laetitia Houot, Adeline Fanni, Sophie Bentzmann, Christophe Bordi

Microbiology (Reading, Engl.) 158:1964-71 (2012)10.1099/mic.0.057059-0

2012
2011
Hacking into bacterial biofilms: a new therapeutic challenge.

Christophe Bordi, Sophie Bentzmann

Annals of Intensive Care 1:19 (2011)10.1186/2110-5820-1-19

2011
1H, 13C and 15N chemical shift assignments of the thioredoxin from the obligate anaerobe Desulfovibrio vulgaris Hildenborough.

Edwige B Garcin, Olivier Bornet, Laetitia Pieulle, Françoise Guerlesquin, Corinne Sebban-Kreuzer

Biomol NMR Assign 5:177-9 (2011)10.1007/s12104-011-9294-5

2011
Two-component regulatory systems in Pseudomonas aeruginosa: an intricate network mediating fimbrial and efflux pump gene expression.

Melissa Sivaneson, Helga Mikkelsen, Isabelle Ventre, Christophe Bordi, Alain Filloux

Mol. Microbiol. 79:1353-66 (2011)10.1111/j.1365-2958.2010.07527.x

2009
Distinct oligomeric forms of the Pseudomonas aeruginosa RetS sensor domain modulate accessibility to the ligand binding site

Florence Vincent, Adam Round, Aline Reynaud, Christophe Bordi, Alain Filloux, Yves Bourne

Environmental Microbiology 12:1775-1786 (2009)10.1111/j.1462-2920.2010.02264.x

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Collaborations

  • National
    • Dr Chantal Iobbi-Nivol, BIP, Marseille
    • Dr Marie Thérèse Giudici-Orticoni, BIP, Marseille

Funding

© 2021 LISM - Laboratoire d'Ingénierie des Systèmes Macromoléculaires - CNRS UMR7255

31 Chemin Joseph Aiguier, 13402 Marseille cedex 20, France