James Sturgis
PU, AMU
Research group: Dynamics and Assembly of membrane proteinsGroup leader
Phone: +33 (0)4 91 16 41 27
Room: IM109
Research Interests
I am interested in developing an interdisciplinary approach to the study of membrane proteininteractions. In particular I have developed in the group an approach that combines molecularbiology and biochemistry with modeling, imagery, and spectroscopy. We use this multidisciplinary approach to study several different systems, notably bacterial photosynthetic membranes, the E. colimembranes and single transmembrane helix proteins such as tyrosine kinase associated receptors.
Publications
2023
2023
Nanodiscs as a novel approach to resolve inter-protein energy transfer within the photosynthetic membrane of purple bacteria
Olivia Fiebig, Dihao Wang, Dvir Harris, Hila Toporik, Yi Ji, Chern Chuang, Muath Nairat, Ashley Tong, John Ogren, Stephanie Hart, Jianshu Cao, James N. Sturgis, Yuval Mazor, Gabriela Schlau-Cohen
Biophysical Journal 122:232a (2023)10.1016/j.bpj.2022.11.1368
2023
Elucidating interprotein energy transfer dynamics within the antenna network from purple bacteria
Dihao Wang, Olivia Fiebig, Dvir Harris, Hila Toporik, Yi Ji, Chern Chuang, Muath Nairat, Ashley Tong, John Ogren, Stephanie Hart, Jianshu Cao, James Sturgis, Yuval Mazor, Gabriela Schlau-Cohen
Proceedings of the National Academy of Sciences of the United States of America 120:e2220477120 (2023)10.1073/pnas.2220477120
2022
2022
Membrane-mediated membrane protein interactions drive membrane protein organization
Yining Jiang, Batiste Thienpont, James N. Sturgis, Jeremy Dittman, Simon Scheuring
Biophysical Journal 121:433a (2022)10.1016/j.bpj.2021.11.608
2022
Membrane-mediated protein interactions drive membrane protein organization
Yining Jiang, Batiste Thienpont, Vinay Sapuru, Richard Hite, Jeremy Dittman, James Sturgis, Simon Scheuring
Nature Communications 13:7373 (2022)10.1038/s41467-022-35202-8
2022
Experimental evidence for long-distance electrodynamic intermolecular forces
Mathias Lechelon, Yoann Meriguet, Matteo Gori, Sandra Ruffenach, Ilaria Nardecchia, Elena Floriani, Dominique Coquillat, Frederic Teppe, Sébastien Mailfert, Didier Marguet, Pierre Ferrier, Luca Varani, James Sturgis, Jeremie Torres, Pettini Marco
Science Advances 8:eabl5855 (2022)10.1126/sciadv.abl5855
2020
2020
Comparison of the Energy-Transfer Rates in Structural and Spectral Variants of the B800–850 Complex from Purple Bacteria
Ashley Tong, Olivia Fiebig, Muath Nairat, Dvir Harris, Marcel Giansily, Aurélia Chenu, James N. Sturgis, Gabriela Schlau-Cohen
Journal of Physical Chemistry B 124:1460-1469 (2020)10.1021/acs.jpcb.9b11899
2020
High-speed atomic force microscopy highlights new molecular mechanism of daptomycin action
Francesca Zuttion, Adai Colom, Stefan Matile, Denes Farago, Frédérique Pompeo, Janos Kokavecz, Anne Galinier, James N. Sturgis, Ignacio Casuso
Nature Communications 11 (2020)10.1038/s41467-020-19710-z
2019
2019
The lipid environment of Escherichia coli Aquaporin Z
Victoria Schmidt, Marlon Sidore, Cherine Bechara, Jean-Pierre Duneau, James N. Sturgis
Biochimica et Biophysica Acta:Biomembranes 1861:431-440 (2019)10.1016/j.bbamem.2018.10.017
2018
2018
Out-of-Equilibrium Collective Oscillation as Phonon Condensation in a Model Protein
Ilaria Nardecchia, Jeremie Torres, Mathias Lechelon, Valeria Giliberti, Michele Ortolani, Philippe Nouvel, Matteo Gori, Yoann Meriguet, Irene Donato, Jordane Preto, Luca Varani, James N. Sturgis, Marco Pettini
Physical Review X 8:031061 (2018)10.1103/physrevx.8.031061
2018
Modifying Styrene-maleic Acid Co-polymer for Studying Lipid Nanodiscs by Direct Fluorescent Labeling
Victoria Schmidt, James N. Sturgis
Bio-protocol 8 (2018)10.21769/BioProtoc.2969
2018
Modifying styrene-maleic acid co-polymer for studying lipid nanodiscs
Victoria Schmidt, James N. Sturgis
Biochimica et Biophysica Acta:Biomembranes 1860:777-783 (2018)10.1016/j.bbamem.2017.12.012
2017
2017
Making Monomeric Aquaporin Z by Disrupting the Hydrophobic Tetramer Interface
Victoria Schmidt, James N. Sturgis
ACS Omega 2:3017-3027 (2017)10.1021/acsomega.7b00261
2017
Lipid perturbation by membrane proteins and the lipophobic effect
James N. Sturgis, Jean-Pierre Duneau, Jonathan Khao
Biochimica et Biophysica Acta:Biomembranes 1859:126-134 (2017)10.1016/j.bbamem.2016.10.014
2016
2016
Making dimers of oligomeric membrane proteins using copper-free click chemistry
James N. Sturgis, Wang Dong
F1000Research 5 (2016)10.12688/f1000research.8676.1
2016
Lipid Environment of Aquaporin Z
Schmidt Victoria, Sidore Marlon, Carrière Fredéric, Duneau Jean-Pierre, James N. Sturgis
Biophysical Journal 110:252a-253a (2016)10.1016/j.bpj.2015.11.1389
2015
2015
The E.coli Aquaporin Z interface : a puzzle?
Victoria Schmidt, Pierre Hubert, James N. Sturgis
Eur. Biophys. J. 44:S217 (2015)
2014
2014
Transmembrane Recognition of the Semaphorin Co-Receptors Neuropilin 1 and Plexin A1 : Coarse-Grained Simulations
Samia Aci-Sèche, Paul Sawma, Pierre Hubert, James N. Sturgis, Dominique Bagnard, Laurent Jacob, Monique Genest, Norbert Garnier
PLoS ONE 9:e97779 (2014)10.1371/journal.pone.0097779
2014
Ultrafast excited state processes in Roseobacter denitrificans antennae: comparison of isolated complexes and native membranes.
Marco Ferretti, Katia Duquesne, James N. Sturgis, Rienk Van Grondelle
Physical Chemistry Chemical Physics 16:26059-66 (2014)10.1039/c4cp02986k
2014
Evidence for new homotypic and heterotypic interactions between transmembrane helices of proteins involved in receptor tyrosine kinase and neuropilin signaling.
Paul Sawma, Lise Roth, Cécile Blanchard, Dominique Bagnard, Gérard Crémel, Emmanuelle Bouveret, Jean-Pierre Duneau, James N. Sturgis, Pierre Hubert
Journal of Molecular Biology 426:4099-111 (2014)10.1016/j.jmb.2014.10.007
2014
The architecture of Rhodobacter sphaeroides chromatophores
Simon Scheuring, Reinat Nevo, Lu-Ning Liu, Stéphanie Mangenot, Dana Charuvi, Thomas Boudier, Valerie Prima, Pierre Hubert, James N. Sturgis, Ziv Reich
Biochimica biophysica acta (BBA) - Bioenergetics 1837:1263-1270 (2014)10.1016/j.bbabio.2014.03.011
2014
Destabilizing Aquaporin Z Assembly: Effects on Structure, Function and Dynamics
Victoria Schmidt, Pierre Hubert, Valerie Prima, James N. Sturgis
Biophysical Journal 108:499a-500a (2014)
2013
2013
Molecular mechanisms of Tau binding to microtubules and its role in microtubule dynamics in live cells.
Gilles Breuzard, Pierre Hubert, Roqiya Nouar, Tiphany De Bessa, François Devred, Pascale Barbier, James N. Sturgis, Vincent Peyrot
Journal of Cell Science 126:2810-9 (2013)10.1242/jcs.120832
2013
Lateral organization of biological membranes: role of long-range interactions.
Jean-Pierre Duneau, James N. Sturgis
Eur. Biophys. J. 42:843-50 (2013)10.1007/s00249-013-0933-x
2012
2012
Structural properties of the tubular appendage spinae from marine bacterium Roseobacter sp. strain YSCB.
A Bernadac, L-F Wu, C-L Santini, C Vidaud, James N. Sturgis, N Menguy, P Bergam, C Nicoletti, T Xiao
Scientific Reports 2:950 (2012)10.1038/srep00950
2012
Characterization of the motion of membrane proteins using high-speed atomic force microscopy
Ignacio Casuso, Jonathan Khao, Mohamed Chami, Perrine Paul-Gilloteaux, Mohamed Husain, Jean-Pierre Duneau, Henning Stahlberg, James N. Sturgis, Simon Scheuring
Nature Nanotechnology 525-529 (2012)10.1038/nnano.2012.109
2012
Complete Lateral and Angular Diffusion and Protein-Protein Interaction Description of a Membrane Protein
Ignacio Casuso, Jean-Pierre Duneau, Mohamed Chami, Perrine Paul-Gilloteaux, Mohamed Husain, Jonathan Khao, Henning Stahlberg, James N. Sturgis, Simon Scheuring
Biophysical Journal 102:413a-414a (2012)10.1016/j.bpj.2011.11.2261
2012
Draft genome sequence of the purple photosynthetic bacterium Phaeospirillum molischianum DSM120, a particularly versatile bacterium.
K Duquesne, Valérie Prima, B Ji, Z Rouy, C Médigue, E Talla, James N. Sturgis
Journal of Bacteriology 194:3559-60 (2012)10.1128/JB.00605-12
2012
Shotgun genome sequence of the large purple photosynthetic bacterium Rhodospirillum photometricum DSM122.
K Duquesne, James N. Sturgis
Journal of Bacteriology 194:2380 (2012)10.1128/JB.00168-12
2011
2011
Molecular origins and consequences of High-800 LH2 in Roseobacter denitrificans.
Katia Duquesne, Cecile Blanchard, James N. Sturgis
Biochemistry 50:6723-9 (2011)10.1021/bi200538j
2011
Structure of a protein-detergent complex: the balance between detergent cohesion and binding.
Jonathan Khao, Jaime Arce-Lopera, James N. Sturgis, Jean-Pierre Duneau
Eur. Biophys. J. 40:1143-55 (2011)10.1007/s00249-011-0745-9
2011
Native architecture of the photosynthetic membrane from Rhodobacter veldkampii.
Lu-Ning Liu, James N. Sturgis, Simon Scheuring
Journal of Structural Biology 173:138-45 (2011)10.1016/j.jsb.2010.08.010
2011
Antagonistic regulation of dgkA and plsB genes of phospholipid synthesis by multiple stress responses in Escherichia coli.
Astrid Wahl, Laetitia My, Romain R. Dumoulin, James N. Sturgis, Emmanuelle Bouveret
Mol. Microbiol. 80:1260-75 (2011)10.1111/j.1365-2958.2011.07641.x
2011
The effects of protein crowding in bacterial photosynthetic membranes on the flow of quinone redox species between the photochemical reaction center and the ubiquinol-cytochrome c2 oxidoreductase.
Kamil Woronowicz, Daniel Sha, Raoul N Frese, James N. Sturgis, Vikas Nanda, Robert A Niederman
Metallomics 3:765-74 (2011)10.1039/c1mt00034a
2009
2009
High-resolution architecture of the outer membrane of the Gram-negative bacteria Roseobacter denitrificans.
Szymon Jarosławski, Katia Duquesne, James N. Sturgis, Simon Scheuring
Molecular Microbiology 74:1211-1222 (2009)10.1111/j.1365-2958.2009.06926.x
2007
2007
Rows of ATP Synthase Dimers in Native Mitochondrial Inner Membranes
Nikolay Buzhynskyy, Pierre Sens, Valérie Prima, James N. Sturgis, Simon Scheuring
Biophysical Journal 93:2870-2876 (2007)10.1529/biophysj.107.109728
2006
2006
Confined diffusion in tubular structures analyzed by fluorescence correlation spectroscopy on a mirror
E. Etienne, P.-F. Lenne, James N. Sturgis, H. Rigneault
Applied optics 45:4497-4507 (2006)10.1364/AO.45.004497
2006
The photosynthetic apparatus of Rhodopseudomonas palustris: Structures and organization.
Simon Scheuring, Rui Pedro Goncalves, Valérie Prima, James N. Sturgis
Journal of Molecular Biology in press:in press (2006)
2001
2001
The TolQ-TolR proteins energize TolA and share homologies with the flagellar motor proteins MotA-MotB
E. Cascales, Roland Lloubès, James N. Sturgis
Molecular Microbiology 42:795-807 (2001)10.1046/j.1365-2958.2001.02673.x
2000
2000
Proton motive force drives the interaction of the inner membrane TolA and outer membrane Pal proteins in Escherichia coli
E. Cascales, Marthe Gavioli, James N. Sturgis, Roland Lloubes
Molecular Microbiology 38:904-915 (2000)10.1046/j.1365-2958.2000.02190.x
2000
1H-13C nuclear magnetic resonance assignment and structural characterization of HIV-1 Tat protein.
Jean-Marie Peloponese, C. Gregoire, S Opi, E Esquieu, James N. Sturgis, E Lebrun, E. Meurs, C Collette, D Olive, M Aubertin, M Witvrow, C Pannecouque, E de Clercq, Cedric Bailly, J. Lebreton, E. Loret
Comptes rendus de l’Académie des sciences. Série III, Sciences de la vie 323:883-94 (2000)10.1016/S0764-4469(00)01228-2