The main objective of our team is to provide information on the molecular mechanisms underlying the assembly, the structure and the mode of action of multiprotein complexes associated with the cell envelope in Gram-negative bacteria, with an emphasis on secretion systems.
We currently develop several lines of study on the type VI secretion system (T6SS), a contractile nanomachine that uses a spring-like mechanism to deliver effectors in target cells. We focus our efforts on understanding the regulation of the expression of T6SS gene clusters, on the structure of the apparatus, and defining how it works and how it propels effectors. An important line of study on the T6SS is to characterize effectors – their activities and targets – and how they are recruited, selected and mounted on the secretion system. A more recent project in our group wishes to define the architecture and the mechanism of action of the type IX secretion system (T9SS), a secretion system restricted to the Bacteroidetes phylum, and involved in pathogenesis and gliding motility. For all these projects, we used a combination of bacterial genetics, molecular biology, physiological tests, structural biology, and fluorescence microscopy.
1 – Architecture and biogenesis of the type VI secretion systems
The type VI secretion system (T6SS) is a multi-subunit machine that is widespread in Gram negative bacteria with an over-representation in Proteobacteria and Bacteroidetes. The T6SS is a contractile machine that uses a spring-like mechanism to propel an effector-loaded needle into the target. The T6SS comprises three sub-complexes: (i) the membrane complex that serves as docking station at the cell envelope and as channel for the passage of the needle during contraction, (ii) the baseplate, which is the assembly platform for (iii) the tail tube/sheath complex.
In the recent years we have focused our efforts on the understanding of the structure and assembly pathway of the membrane and baseplate subcomplexes. We have also identified subunits that coordinate or control tail tube/sheath assembly, termination or contraction. We collaborate with several colleagues in France or abroad, notably the group of R. Fronzes for electron microscopy structural studies.
2 – Activity and delivery of type VI secretion toxins
The type VI secretion system (T6SS) injects toxins to either prokaryotic or eukaryotic cells and is thus directly involved in virulence, and/or participate to clear and colonize niches by killing bacterial competitors. The competitive advantage provided by T6SS on intra and inter-species bacterial interaction in the gut and thus on the composition of the microbiota was recently highlighted. Our goal is to identify and determine the mode of action and the targets of T6SS effector toxins, using pathogenic Escherichia coli and related species as models. We also want to understand how T6SS effectors are loaded on the T6SS machine for delivery and how they are activated in the target cell. Specifically, in the recent years, we characterized an antibacterial phospholipase toxin of enteroaggregative E. coli and its immunity protein Tli1. Three Tle1 toxins interact directly with VgrG, the needle spike of the machine, for delivery in the periplasm of target cells.
3 – The Type IX secretion in pathogenesis and gliding motility
The phylum Bacteroidetes is under-studied despite many of its members being important pathogens and commensals. These bacteria distinguish themselves by using the type IX secretion system (T9SS). This machinery is composed of at least 18 proteins assembled to secrete effectors supporting a wide variety of functions.
Notably, in Porphyromonas gingivalis, the main microorganism responsible for periodontitis, the T9SS secretes a cocktail of adhesins and proteases called gingipains to promote tissue invasion.
Several Bacteroidetes members also use the T9SS for gliding. This motility process relies on adhesins translocated to the cell surface and set into motion by the T9SS. Using a multidisciplinary approach, we are dedicated to understand the T9SS at multiple scales, from the organization of the machinery, the secretion pathway, the biological functions, to the link with gliding. We collaborate with Alain Roussel at AFMB and have recently biochemically and structurally characterized key subunits and subcomplexes.
Members
Post doctoral researchers
- Gauthier Dangla-Pélissier (2020-2023)
- Dukas Jurenas (2019-2022)
- Victoria Schmidt (2018-2019)
- Amaury Payelleville (2019)
- Erwan Gueguen (2010-2012)
PhD fellows
- Romain Debiton Ph.D 2023. Thesis entitled “Localization of the T6SS”.
- Solène Beauvois Ph.D 2023. Thesis entitled “Architecture et fonction du complexe de pointe PAAR-VgrG-Tle1 du système de sécrétion de type VI d’EAEC”.
- Boris Taillefer Ph.D 2023. Thesis entitled “Heterogeneity of Type VI secretion system”.
- Ignacio Lunar Silva Ph.D 2021. Thesis entitled “Type IX secretion: characterization of effector transport pathway”.
- Yoann Santin Ph.D 2021. Thesis entitled “Molecular characterization of the type VI secretion system size-control mechanism”.
- Yassine Cherrak Ph.D 2020. entitled “Caractérisation structurale et inhibition d’une nano-machine impliquée dans la compétition bactérienne : le T6SS”.
- Maxence Vincent Ph.D 2019. Thesis entitled “From cell surface anchoring to gliding motility: Structural and functional characterization of the type IX secretion system GldKLMN core complex”.
- Nicolas Flaugnatti Ph.D 2018. Thesis entitled “Le système de sécrétion de type VI: caractérisation et mécanisme de transport de Tle1, un effecteur antibactérien de type phospholipase”.
- Laureen Logger Ph.D 2017. Thesis entitled “The type VI secretion membrane complex: the central role of TssM for assembly and for baseplate recruitment”.
- Abdelrahim Zoued Ph.D 2015. Thesis entitled “Biogenesis and membrane anchoring of the type VI secretion contractile tail”.
- Yannick R. Brunet. Ph.D 2013. Thesis entitled “Structure and function of the type VI secretion system tail”.
- Marie-Stéphanie Aschtgen Ph.D 2011. Thesis entitled “Etude du système de sécrétion de type VI chez Escherichia coli enter-agrégatif. Caractérisation d’un sous-complexe d’ancrage membranaire”.
Technical staff
- Audrey Tempier (2021-2023)
- Caterina Comas (2020-2023)
- Marie Boyer (2020-2023)
- Sophie Gibault (2022)
- Etienne Vanlioglu (2018-2021)
- Xiang Y. Zhang (2011-2013)
B.Sc. and M. Sc undergraduates
Yannick Brunet (AMU, B.Sc., 2008)
Marie Guérin (AMU, M1, 2010)
Abir Benzegoutta (AMU, M1, 2010)
Abdelrahim Zoued (AMU, M1, 2010; M2 2011)
Quentin d’Amalric (AMU, M1, 2011)
Delphine Prévost (AMU, ESIL-PolyTech, 2011)
Valentin Tutagata (BTS, 2012)
Laureen Logger (AMU, M1, 2012; M2, 2012)
Seddik Harchouni (AMU, B.Sc., 2012)
Valentine Lensi (AMU, L2, 2012)
Charlotte Gaviard (AMU, B.Sc., 2012)
Lia Freier (B.Sc. Erasmus, Tuebingen Univ., Germany, 2013)
Jean-Charles Cancel (AMU, L2, 2013)
Emma Long (AMU, ESIL-PolyTech, 2013)
Guillaume Urbain (AMU, M1, 2013)
Aurélie Fosses (AMU, M1, 2013)
Yann Dauchy (AMU, B.Sc., 2013)
Jordi Zamarreno-Beas (AMU, B.Sc., 2013)
Anant Kumar Singh (Indian Research Scholar fellow, 2013)
Mickaël Canestrari (AMU, B.Sc., 2013)
Elodie Pagès (AMU, ESIL-PolyTech, 2014)
Maxence Vincent (AMU, M1, 2014)
Yoann Santin (AMU, L2, 2014)
Doriane Delafosse (AMU, B.Sc., 2014)
Alexandre Espana (AMU, B.Sc., 2014)
Nicolas Flaugnatti (AMU, M.Sc., 2014)
Marcus Höring (M.Sc. Erasmus, Regensburg Univ., Germany, 2014)
Corentin Baussier (AMU, L1 Bio, 2015)
Sam Cascales (3ème, 2015)
Kevin Arnould (AMU, ESIL-PolyTech, 2015)
Sarah Tjaden (Visiting PhD, Münster, Germany, 2015 & 2016)
Vincent Palomo (AMU, L2, 2015)
Tristan Pommier (AMU, B.Sc., 2015)
Maxence Durand (3ème, 2015)
Hugo Perez (3ème, 2016)
Chakib Khenafra (AMU, L1, 2016)
Alexis Dogliani (AMU, M1, 2016)
Viet Anh Nguyen (M.Sc., USTH, Vietnam, 2016)
Chloé Cassaro (University of Glascow, Scotland, 2016)
Mathias Gallique (Visiting Ph.D, Evreux, 2016)
Wanassa Béroual (AMU, B.Sc., 2016)
Théo Dubois (2de, 2017)
Stéphane Alibar (AMU, M1, 2017; M2, 2018)
Melvin Renault (AMU, B.Sc., 2017)
Claire Camy (AMU, B.Sc., 2017)
Camille Garcia (AMU, M2, 2018)
Romain Debiton (B.Sc., 2018; M1, 2019)
Ryan Ellison (Exeter College, Oxford University, Laidlaw scholar, 2018)
Mehdi Zouanbi (AMU, B. Sc., 2018)
Victoria Sgoluppi (AMU, B.Sc., 2018 and 2019)
Zoé Cascales (3ème, 2019)
Benjamin Bagnis (AMU, M1, 2019)
Thibault Bongiovanni (AMU, M1, 2019)
Baptiste Piguet-Ruinet (AMU, L2, 2019)
Ashleigh Morgan (Exeter College, Oxford University, 2019)
Samuel Carien (AMU, L2, 2019)
Tiffany Lihoreau (AMU, ESIL-PolyTech, 2019)
Camille Herrou (AMU, L3, 2020)
Jonas Desjardins (AMU, L3, 2020)
Yousra Onrifani (BTS, 2020)
Fabio Meraihi (AMU, L3, 2020-2021)
Jonathan Julien (AMU, DFGSM3, 2020-2021)
Margot Grimonpont (AMU, M1 MIF, 2021)
Morgane Brigitte (AMU, M1 MIF, 2021)
Jérémy Mom (AMU, M1 MIF, 2021)
Yvann Bourigault (Visiting PhD, Evreux, 2021)
Sophie Du Crest (BTS, 2021)
Floriane Vernay (AMU, L2, 2021)
Pierre Liénart (AMU, L2, 2021)
Noa Guzzi (AMU, L3, 2021)
Julien Giraud (AMU, L3, 2021)
Marine Giraud (AMU, L1, 2022)
Thomas Zur Nedden (Université Libre de Bruxelles, M2, 2022)
Edinia Moraes Soreira (BTS, 2022)
Solé Humbert-Massasso (3ème, 2022)
Camille Field-Ize (3ème, 2022)
Nathan Gombeaud-Saintonge (AMU, ESIL-PolyTech, 2022)
Thomas Vivet (AMU, L2, 2022)
Sofia Hatych (AMU, L3, 2022)
Sirui Li (Wuhan University of Technology, China, M2, 2022)
Maja Andiel (L3 ERASMUS, Tübingen, Germany, 2022)
Margot Dessartine (Visiting Ph.D, Sherbrooke, Canada, 2022)
Corana Nobile (AMU, L1, 2023)
Chloé Piras (AMU, M1 MIF, 2023)
Bastien Charrat (AMU, M1 MIF, 2023)
Maximilien Rouzaud (AMU, M1 BSG, 2023)
Louise-Elisabeth Dintilhac (Université de Rennes, M2, 2023)
Téa Massaroni (3ème, 2023)
Léa Duchemin (AMU, L3, 2023)
Martin Durrmeyer (AMU, M1 MIF, 2023)
Atakan Kabtan (Université Libre de Bruxelles, M2, 2023)
Lou Marchi (INSA Strasbourg, 2023)
Mathias Cardin (AMU, L2, 2023)
Sylvain Lacroix (AMU, L3, 2023)
Thibault Delorbe (AMU, L2, 2014)
Stanislas Padilla (AMU, L2, 2024)
Publications
2024
Type 1 fimbriae-mediated collective protection against type 6 secretion system attacks
Margot Marie Dessartine, Artemis Kosta, Thierry Doan, Éric Cascales, Jean-Philippe Côté
mBio e0255323 (2024)10.1128/mbio.02553-23
2024
The type VI secretion system of Stenotrophomonas rhizophila CFBP13503 limits the transmission of Xanthomonas campestris pv. campestris 8004 from radish seeds to seedlings
Tiffany Garin, Chrystelle Brin, Anne Préveaux, Agathe Brault, Martial Briand, Marie Simonin, Matthieu Barret, Laure Journet, Alain Sarniguet
Molecular Plant Pathology 25:e13412 (2024)10.1111/mpp.13412
2024
Structure–function analysis of PorXFj, the PorX homolog from Flavobacterium johnsioniae, suggests a role of the CheY-like domain in type IX secretion motor activity
Mariotte Zammit, Julia Bartoli, Christine Kellenberger, Pauline Melani, Alain Roussel, Eric Cascales, Philippe Leone
Scientific Reports 14:6577 (2024)10.1038/s41598-024-57089-9
2023
Characterization of a (p)ppApp Synthetase Belonging to a New Family of Polymorphic Toxin Associated with Temperate Phages
Julia Bartoli, Audrey C Tempier, Noa L Guzzi, Chloé M Piras, Eric Cascales, Julie P M Viala
Journal of Molecular Biology 435:168282 (2023)10.1016/j.jmb.2023.168282
2023
The tip protein PAAR is required for the function of the type VI secretion system
Solène G Beauvois, Nicolas Flaugnatti, Marianne Ilbert, Marie Boyer, Esther Gavello-Fernandez, Rémi Fronzes, Dukas Jurėnas, Laure Journet
Microbiology Spectrum (2023)10.1128/spectrum.01478-23
2023
Pseudomonas fluorescens MFE01 delivers a putative type VI secretion amidase that confers biocontrol against the soft‐rot pathogen Pectobacterium atrosepticum
Yvann Bourigault, Charly A Dupont, Jonas B Desjardins, Thierry Doan, Mathilde Bouteiller, Hugo Le Guenno, Sylvie Chevalier, Corinne Barbey, Xavier Latour, Eric Cascales, Annabelle Merieau
Environmental Microbiology (2023)10.1111/1462-2920.16492
2023
Activity and Crystal Structure of the Adherent-Invasive Escherichia coli Tle3/Tli3 T6SS Effector/Immunity Complex Determined Using an AlphaFold2 Predicted Model
Thi Thu Hang Le, Christine Kellenberger, Marie Boyer, Pierre Santucci, Nicolas Flaugnatti, Eric Cascales, Alain Roussel, Stéphane Canaan, Laure Journet, Christian Cambillau
International Journal of Molecular Sciences 24:1740 (2023)10.3390/ijms24021740
2023
The morphogenic protein CopD controls the spatio-temporal dynamics of PBP1a and PBP2b in Streptococcus pneumoniae
Cassandra Lenoir, Anaïs Pelletier, Sylvie Manuse, Hugo Millat, Adrien Ducret, Anne Galinier, Thierry Doan, Christophe Grangeasse
mBio (2023)10.1128/mbio.01411-23
2023
A journey with type IX secretion system effectors: selection, transport, processing and activities
Maëlle Paillat, Ignacio Lunar Silva, Eric Cascales, Thierry Doan
Microbiology 169 (2023)10.1099/mic.0.001320
2023
No fitness cost entailed by type VI secretion system synthesis, assembly, contraction, or disassembly in enteroaggregative Escherichia coli
Boris Taillefer, Julien F Giraud, Eric Cascales
Journal of Bacteriology 205 (2023)10.1128/jb.00357-23
2023
Qualitative and Quantitative Methods to Measure Antibacterial Activity Resulting from Bacterial Competition
Boris Taillefer, Marie Grandjean, Julien Herrou, Donovan Robert, Tam Mignot, Corinne Sebban-Kreuzer, Eric Cascales
Bio-protocol 13:e4706 (2023)10.21769/BioProtoc.4706
2023
Coevolution-guided mapping of the Type VI secretion membrane complexbaseplate interface
Etienne Vanlioğlu, Yoann Santin, Isaac Filella-Merce, Riccardo Pellarin, Eric Cascales
Journal of Molecular Biology 435:167918 (2023)10.1016/j.jmb.2022.167918
2022
Structural and functional analyses of the Porphyromonas gingivalis type IX secretion system PorN protein
Olivier Fuchsbauer, Ignacio Lunar Silva, Eric Cascales, Alain Roussel, Philippe Leone
Journal of Biological Chemistry 298:101618 (2022)10.1016/j.jbc.2022.101618
2022
Protein Interactome Analysis of the Type IX Secretion System Identifies PorW as the Missing Link between the PorK/N Ring Complex and the Sov Translocon
Dhana Gorasia, Ignacio Lunar Silva, Catherine Butler, Maïalène Chabalier, Thierry Doan, Eric Cascales, Paul Veith, Eric Reynolds
Microbiology Spectrum e0160221 (2022)10.1128/spectrum.01602-21
2022
Salmonella antibacterial Rhs polymorphic toxin inhibits translation through ADP-ribosylation of EF-Tu P-loop
Dukas Jurėnas, Martial Rey, Deborah Byrne, Julia Chamot-Rooke, Laurent Terradot, Eric Cascales
Nucleic Acids Research 50:13114-13127 (2022)10.1093/nar/gkac1162
2022
Membrane fission during bacterial spore development requires cellular inflation driven by DNA translocation
Ane Landajuela, Martha Braun, Alejandro Martínez-Calvo, Christopher D.A. Rodrigues, Carolina Gomis Perez, Thierry Doan, David Rudner, Ned Wingreen, Erdem Karatekin
Current Biology - CB 32:4186-4200.e8 (2022)10.1016/j.cub.2022.08.014
2022
A Unique Bacterial Secretion Machinery with Multiple Secretion Centers
Liqiang Song, John Perpich, Chenggang Wu, Thierry Doan, Zuzanna Nowakowska, Jan Potempa, Peter Christie, E. Cascales, Richard Lamont, Bo Hu
Proceedings of the National Academy of Sciences of the United States of America 119 (2022)10.1073/pnas.2119907119
2022
The Antibacterial Type VII Secretion System of Bacillus subtilis: Structure and Interactions of the Pseudokinase YukC/EssB
Matteo Tassinari, Thierry Doan, Marco Bellinzoni, Maïalene Chabalier, Mathilde Ben-Assaya, Mariano Martinez, Quentin Gaday, Pedro Alzari, Eric Cascales, Rémi Fronzes, Francesca Gubellini
mBio e0013422 (2022)10.1128/mbio.00134-22
2022
The Type IX Secretion System and Its Role in Bacterial Function and Pathogenesis
P.D. Veith, M.D. Glew, D.G. Gorasia, E.C. Reynolds, E. Cascales
Journal of Dental Research 101:374-383 (2022)10.1177/00220345211051599
2022
Dynamic proton-dependent motors power type IX secretion and gliding motility in Flavobacterium
Maxence Vincent, Caterina Comas Hervada, Corinne Sebban-Kreuzer, Hugo Le Guenno, Maïalène Chabalier, Artemis Kosta, Françoise Guerlesquin, Tâm Mignot, Mark Mcbride, Thierry Doan, E. Cascales
PLoS Biology 20:e3001443 (2022)10.1371/journal.pbio.3001443
2021
The Azospirillum brasilense Type VI secretion system promotes cell aggregation, biocontrol protection against phytopathogens and attachment to the microalgae Chlorella sorokiniana
Fabricio Cassan, Anahí Coniglio, Edgar Amavizca, Guillermo Maroniche, E. Cascales, Yoav Bashan, Luz De‐bashan
Environmental Microbiology (2021)10.1111/1462-2920.15749
2021
The Azospirillum brasilense type VI secretion system promotes cell aggregation, biocontrol protection against phytopathogens and attachment to the microalgae Chlorella sorokiniana
Fabricio Cassan, Anahí Coniglio, Edgar Amavizca, Guillermo Maroniche, E. Cascales, Yoav Bashan, Luz De‐bashan
Environmental Microbiology 23:6257-6274 (2021)10.1111/1462-2920.15749
2021
Dual Regulation of Phosphatidylserine Decarboxylase Expression by Envelope Stress Responses
Yasmine Hassoun, Julia Bartoli, Astrid Wahl, Julie Viala, Emmanuelle Bouveret
Frontiers in Molecular Biosciences 8 (2021)10.3389/fmolb.2021.665977
2021
Activity, delivery and diversity of Type VI secretion effectors
Dukas Jurėnas, Laure Journet
Molecular Microbiology 115:383-394 (2021)10.1111/mmi.14648
2021
Mounting, structure and autocleavage of a type VI secretion-associated Rhs polymorphic toxin
Dukas Jurėnas, Leonardo Talachia Rosa, Martial Rey, Julia Chamot-Rooke, Rémi Fronzes, E. Cascales
Nature Communications 12 (2021)10.1038/s41467-021-27388-0
2021
Photorhabdus antibacterial Rhs polymorphic toxin inhibits translation through ADP-ribosylation of 23S ribosomal RNA
Dukas Jurėnas, Amaury Payelleville, Mohammad Roghanian, Kathryn Turnbull, Alain Givaudan, Julien Brillard, Vasili Hauryliuk, E. Cascales
Nucleic Acids Research 49:8384-8395 (2021)10.1093/nar/gkab608
2021
T6SS: killing two bugs with one stone
Dukas Jurėnas, E. Cascales
Trends in Microbiology (2021)10.1016/j.tim.2021.10.006
2021
FisB relies on homo-oligomerization and lipid binding to catalyze membrane fission in bacteria
Ane Landajuela, Martha Braun, Christopher Rodrigues, Alejandro Martínez-Calvo, Thierry Doan, Florian Horenkamp, Anna Andronicos, Vladimir Shteyn, Nathan Williams, Chenxiang Lin, Ned Wingreen, David Rudner, Erdem Karatekin
PLoS Biology 19:e3001314 (2021)10.1371/journal.pbio.3001314
2021
Molecular Strategies Underlying Porphyromonas gingivalis Virulence
Ignacio Lunar Silva, E. Cascales
Journal of Molecular Biology 433:166836 (2021)10.1016/j.jmb.2021.166836
2021
Anchoring the T6SS to the cell wall: Crystal structure of the peptidoglycan binding domain of the TagL accessory protein
van Son Nguyen, Silvia Spinelli, E. Cascales, Alain Roussel, Christian Cambillau, Philippe Leone
PLoS ONE 16:e0254232 (2021)10.1371/journal.pone.0254232
2021
A Tad-like apparatus is required for contact-dependent prey killing in predatory social bacteria
Sofiene Seef, Julien Herrou, Paul de Boissier, Laetitia My, Gael Brasseur, Donovan Robert, Rikesh Jain, Romain Mercier, Eric Cascales, Bianca Habermann, Tâm Mignot
eLife 10 (2021)10.7554/eLife.72409
2020
SlyA Transcriptional Regulator Is Not Directly Affected by ppGpp Levels
Julia Bartoli, Julie Viala, Emmanuelle Bouveret
Frontiers in Microbiology 11:1856 (2020)10.3389/fmicb.2020.01856
2020
Fur -Dam regulatory interplay at an internal promoter of the enteroaggregative Escherichia coli Type VI secretion sci1 gene cluster
Yannick R Brunet, Christophe S Bernard, E. Cascales
Journal of Bacteriology (2020)
2020
Structural basis for loading and inhibition of a bacterial T6 SS phospholipase effector by the VgrG spike
Nicolas Flaugnatti, Chiara Rapisarda, Martial Rey, Solène G Beauvois, Viet Anh Nguyen, Stéphane Canaan, Eric Durand, Julia Chamot‐rooke, E. Cascales, Rémi Fronzes, Laure Journet
EMBO Journal 39:e104129 (2020)10.15252/embj.2019104129
2019
Deciphering the specific interaction between the acyl carrier protein IacP and the T3SS‐major hydrophobic translocator SipB from Salmonella
Mickaël J Canestrari, Bastien Serrano, Julia Bartoli, Valerie Prima, Olivier Bornet, Rémy Puppo, Emmanuelle Bouveret, Francoise Guerlesquin, Julie Viala
FEBS Letters (2019)10.1002/1873-3468.13593
2019
Structure and Activity of the Type VI Secretion System
Yassine Cherrak, Nicolas Flaugnatti, Eric Durand, Laure Journet, E. Cascales
Microbiology Spectrum 7 (2019)10.1128/microbiolspec.PSIB-0031-2019
2019
In situ and high-resolution Cryo-EM structure of the Type VI secretion membrane complex
Chiara Rapisarda, Yassine Cherrak, Romain Kooger, Victoria Schmidt, Riccardo Pellarin, Laureen Logger, E. Cascales, Martin Pilhofer, Eric Durand, Rémi Fronzes
EMBO Journal 38 (2019)10.15252/embj.2018100886
2019
Cell width dictates Type VI secretion tail length
Yoann G Santin, Thierry Doan, Laure Journet, E. Cascales
Current Biology - CB (2019)10.1016/j.cub.2019.08.058
2019
Role and recruitment of the TagL peptidoglycan-binding protein during Type VI secretion system biogenesis
Yoann G Santin, Claire Camy, Abdelrahim Zoued, Thierry Doan, Marie-Stéphanie Aschtgen, E. Cascales
Journal of Bacteriology (2019)10.1128/JB.00173-19
2018
Antibacterial Weapons: Targeted Destruction in the Microbiota
Benoit Chassaing, E. Cascales
Trends in Microbiology 26:329-338 (2018)10.1016/j.tim.2018.01.006
2018
Biogenesis and structure of a type VI secretion baseplate
Yassine Cherrak, Chiara Rapisarda, Riccardo Pellarin, Guillaume Bouvier, Benjamin Bardiaux, Fabrice Allain, Christian Malosse, Martial Jean-Pierre Rey, Julia Chamot-Rooke, E. Cascales, Rémi Fronzes, Eric Durand
Nature Microbiology 3:1404-1416 (2018)10.1038/s41564-018-0260-1
2018
Structure–Function Analysis of the C-Terminal Domain of the Type VI Secretion TssB Tail Sheath Subunit
Badreddine Douzi, Laureen Logger, Silvia Spinelli, Stephanie Blangy, Christian Cambillau, E. Cascales
Journal of Molecular Biology 430:297-309 (2018)10.1016/j.jmb.2017.11.015
2018
Type IX secretion system PorM and gliding machinery GldM form extended arches spanning the periplasmic space
Philippe Leone, Jennifer Roche, Maxence S Vincent, Quang Hieu Tran, Aline Desmyter, E. Cascales, Christine Kellenberger, Christian Cambillau, Alain Roussel
Nature Communications 9 (2018)10.1038/s41467-017-02784-7
2018
Towards a complete structural deciphering of Type VI secretion system
van Son Nguyen, Badreddine Douzi, Eric Durand, Alain Roussel, E. Cascales, Christian Cambillau
Current Opinion in Structural Biology 49:77-84 (2018)10.1016/j.sbi.2018.01.007
2018
The gp27-like Hub of VgrG Serves as Adaptor to Promote Hcp Tube Assembly
Melvin G Renault, Jordi Zamarreño Beas, Badreddine Douzi, Maïalène Chabalier, Abdelrahim Zoued, Yannick R Brunet, Christian Cambillau, Laure Journet, E. Cascales
Journal of Molecular Biology 430:3143-3156 (2018)10.1016/j.jmb.2018.07.018
2018
In vivo TssA proximity labeling reveals temporal interactions during Type VI secretion 1 biogenesis and TagA, a protein that stops and holds the sheath.
Yoann G Santin, Thierry Doan, Régine Lebrun, Leon Espinosa, Laure Journet, E. Cascales
Nature Microbiology 3:1304-1313 (2018)10.1038/s41564-018-0234-3
2018
Tryptophan-mediated Dimerization of the TssL Transmembrane Anchor Is Required for Type VI Secretion System Activity
Abdelrahim Zoued, Jean-Pierre Duneau, Eric Durand, Alexandre España, Laure Journet, Françoise Guerlesquin, E. Cascales
Journal of Molecular Biology 430:987-1003 (2018)10.1016/j.jmb.2018.02.008
2017
The Long Hunt for pssR —Looking for a Phospholipid Synthesis Transcriptional Regulator, Finding the Ribosome
J. Bartoli, L. My, Lucid Belmudes, Yohann Coute, Julie Viala, E. Bouveret
Journal of Bacteriology 199 (2017)10.1128/JB.00202-17
2017
Inside the Chamber of Secrets of the Type III Secretion System
E. Cascales
Cell 168:949-951 (2017)10.1016/j.cell.2017.02.028
2017
Microbiology: And Amoebophilus Invented the Machine Gun!
E. Cascales
Current Biology - CB 27:R1170-R1173 (2017)10.1016/j.cub.2017.09.025
2017
Type VI secretion TssK baseplate protein exhibits structural similarity with phage receptor-binding proteins and evolved to bind the membrane complex
van Son Nguyen, Laureen Logger, Silvia Spinelli, Pierre Legrand, Thi Thanh Huyen Pham, Thi Trang Nhung Trinh, Yassine Cherrak, Abdelrahim Zoued, Aline Desmyter, Eric Durand, Alain Roussel, Christine Kellenberger, E. Cascales, Christian Cambillau
Nature Microbiology 2:17103 (2017)10.1038/nmicrobiol.2017.103
2017
Domestication of a housekeeping transglycosylase for assembly of a Type VI secretion system
Yoann G Santin, E. Cascales
EMBO Reports 18:138-149 (2017)10.15252/embr.201643206
2017
The iron-sulfur cluster sensor IscR is a negative regulator of Spi1 Type III Secretion System in Salmonella enterica Cellular Microbiology
Alexandra Vergnes, Julie Viala, Ouadah‐tsabet Rabah, Bérengère Pocachard, Laurent Loiseau, Stephane Meresse, Frédéric Barras, Laurent Aussel
Cellular Microbiology 19:e12680 (2017)10.1111/cmi.12680
2017
Acylation of the Type 3 Secretion System Translocon Using a Dedicated Acyl Carrier Protein
Julie Viala, Valerie Prima, Remy Puppo, Rym Agrebi, Mickael J. Canestrari, Sabrina Lignon, Nicolas Chauvin, Stephane Meresse, Tam Mignot, Regine Lebrun, Emmanuelle Bouveret
PLoS Genetics 13 (2017)10.1371/journal.pgen.1006556
2017
Characterization of the Porphyromonas gingivalis Type IX Secretion Trans-envelope PorKLMNP Core Complex
Maxence S Vincent, Mickaël J Canestrari, Philippe Leone, Julien Stathopulos, Bérengère Ize, Abdelrahim Zoued, Christian Cambillau, Christine Kellenberger, Alain Roussel, E. Cascales
Journal of Biological Chemistry 292:3252-3261 (2017)10.1074/jbc.M116.765081
2017
TssA: The cap protein of the Type VI secretion system tail
Abdelrahim Zoued, Eric Durand, Yoann G Santin, Laure Journet, Alain Roussel, Christian Cambillau, E. Cascales
BioEssays 39 (2017)10.1002/bies.201600262
2016
Meeting report: Adaptation and communication of bacterial pathogens
Laurent Aussel, Carmen Beuzón, E. Cascales
Virulence 7:481-490 (2016)10.1080/21505594.2016.1152441
2016
Polyamino-Isoprenic Derivatives Block Intrinsic Resistance of P. aeruginosa to Doxycycline and Chloramphenicol In Vitro
Diane Borselli, Aurélie Lieutaud, Hélène Thefenne, Eric Garnotel, Jean-Marie Pagès, Jean Michel Brunel, Jean-Michel Bolla, E. Cascales
PLoS ONE 11:e154490 (2016)10.1371/journal.pone.0154490
2016
Structure and specificity of the Type VI secretion system ClpV-TssC interaction in enteroaggregative Escherichia coli
Badreddine Douzi, Yannick R Brunet, Silvia Spinelli, Valentine Lensi, Stephanie Blangy, Anant Kumar, Laure Journet, Pierre Legrand, E. Cascales, Christian Cambillau
Scientific Reports 6 (2016)10.1038/srep34405
2016
A phospholipase A 1 antibacterial Type VI secretion effector interacts directly with the C-terminal domain of the VgrG spike protein for delivery
Nicolas Flaugnatti, Thi Thu Hang Le, Stéphane Canaan, Marie-Stéphanie Aschtgen, Vân Nguyên, Stephanie Blangy, Christine Kellenberger, Alain Roussel, Christian Cambillau, E. Cascales, Laure Journet
Molecular Microbiology 99:1099-1118 (2016)10.1111/mmi.13292
2016
The Type VI Secretion System in Escherichia coli and Related Species
Laure Journet, E. Cascales
EcoSal Plus 7 (2016)10.1128/ecosalplus.ESP-0009-2015
2016
Molecular Dissection of the Interface between the Type VI Secretion TssM Cytoplasmic Domain and the TssG Baseplate Component
Laureen Logger, Marie-Stéphanie Aschtgen, Marie Guérin, E. Cascales, Eric Durand
Journal of Molecular Biology 428:4424-4437 (2016)10.1016/j.jmb.2016.08.032
2016
Coexpression of Escherichia coli obgE, Encoding the Evolutionarily Conserved Obg GTPase, with Ribosomal Proteins L21 and L27.
Rim Maouche, Hector L Burgos, Laetitia My, Julie Viala, Richard L Gourse, Emmanuelle Bouveret
Journal of Bacteriology 198:1857-67 (2016)10.1128/JB.00159-16
2016
Salmonella Typhimurium utilizes a T6SS-mediated antibacterial weapon to establish in the host gut
Thibault G Sana, Nicolas Flaugnatti, Kyler A Lugo, Lilian H Lam, Amanda Jacobson, Virginie Baylot, Eric Durand, Laure Journet, E. Cascales, Denise M Monack
Proceedings of the National Academy of Sciences of the United States of America 113:E5044-E5051 (2016)10.1073/pnas.1608858113
2016
The PorX Response Regulator of the Porphyromonas gingivalis PorXY Two-Component System Does Not Directly Regulate the Type IX Secretion Genes but Binds the PorL Subunit
Maxence S Vincent, Eric Durand, E. Cascales
Frontiers in Cellular and Infection Microbiology 6 (2016)10.3389/fcimb.2016.00096
2016
Priming and polymerization of a bacterial contractile tail structure. 1" 2" 17
Abdelrahim Zoued, Eric Durand, Yannick R Brunet, Silvia Spinelli, Badreddine Douzi, Mathilde Guzzo, Nicolas Flaugnatti, Pierre Legrand, Laure Journet, Rémi Fronzes, Tam Mignot, Christian Cambillau, E. Cascales
Nature 531:59-63 (2016)10.1038/nature17182
2016
Structure–Function Analysis of the TssL Cytoplasmic Domain Reveals a New Interaction between the Type VI Secretion Baseplate and Membrane Complexes
Abdelrahim Zoued, Chloé J Cassaro, Eric Durand, Badreddine Douzi, Alexandre España, Christian Cambillau, Laure Journet, E. Cascales
Journal of Molecular Biology 428:4413-4423 (2016)10.1016/j.jmb.2016.08.030
2015
H-NS Silencing of the Salmonella Pathogenicity Island 6-Encoded Type VI Secretion System Limits Salmonella enterica Serovar Typhimurium Interbacterial Killing
Yannick Brunet, Ahmad Khodr, Laureen Logger, Laurent Aussel, Tâm Mignot, Sylvie Rimsky, E. Cascales
Infection and Immunity 83:2738-2750 (2015)10.1128/IAI.00198-15
2015
The Type VI Secretion TssEFGK-VgrG Phage-Like Baseplate Is Recruited to the TssJLM Membrane Complex via Multiple Contacts and Serves As Assembly Platform for Tail Tube/Sheath Polymerization
Yannick Brunet, Abdelrahim Zoued, Frédéric Boyer, Badreddine Douzi, E. Cascales
PLoS Genetics 11:e1005545 (2015)10.1371/journal.pgen.1005545
2015
Biogenesis and structure of a Type VI secretion membrane core complex
Eric Durand, van Son Nguyen, Abdelrahim Zoued, Laureen Logger, Gerard Pehau-Arnaudet, Marie-Stéphanie Aschtgen, Silvia Spinelli, Aline Desmyter, Benjamin Bardiaux, Annick Dujeancourt, Alain Roussel, Christian Cambillau, E. Cascales, Rémi Fronzes
Nature 523:555-560 (2015)10.1038/nature14667
2015
Reassessment of the Genetic Regulation of Fatty Acid Synthesis in Escherichia coli: Global Positive Control by the Dual Functional Regulator FadR
L My, N Ghandour Achkar, Julie Viala, Emmanuelle Bouveret
Journal of Bacteriology 197:1862-72 (2015)10.1128/JB.00064-15
2015
Inhibition of Type VI Secretion by an Anti-TssM Llama Nanobody
van Son Nguyen, Laureen Logger, Silvia Spinelli, Aline Desmyter, Thi Thu Hang Le, Christine Kellenberger, Badreddine Douzi, Eric Durand, Alain Roussel, E. Cascales, Christian Cambillau
PLoS ONE 10:1-14 (2015)
2015
Production, crystallization and X-ray diffraction analysis of a complex between a fragment of the TssM T6SS protein and a camelid nanobody
van Son Nguyen, Silvia Spinelli, Aline Desmyter, Thi Thu Hang Le, Christine Kellenberger, E. Cascales, Christian Cambillau, Alain Roussel
Acta crystallographica Section F : Structural biology communications [2014-...] 71:266-271 (2015)10.1107/S2053230X15000709
2015
Crystallization and preliminary X-ray analysis of the C-terminal fragment of PorM, a subunit of the Porphyromonas gingivalis type IX secretion system
Julien Stathopulos, Christian Cambillau, E. Cascales, Alain Roussel, Philippe Leone
Acta crystallographica Section F : Structural biology communications [2014-...] 71:71-74 (2015)10.1107/S2053230X1402559X
2014
Anchoring the type VI secretion system to the peptidoglycan: TssL, TagL, TagP... what else?
Marie-Stéphanie Aschtgen, Mark Thomas, E. Cascales
Virulence 1:535-540 (2014)10.4161/viru.1.6.13732
2014
Type VI secretion and bacteriophage tail tubes share a common assembly pathway.
Yannick R Brunet, Jérôme Hénin, Hervé H. Celia, E. Cascales
EMBO Reports 15:315-21 (2014)10.1002/embr.201337936
2014
Type VI secretion and bacteriophage tail tubes share a common assembly pathway
Yannick Brunet, Jérôme Hénin, Hervé Celia, E. Cascales
EMBO Reports 15:315-321 (2014)10.1002/embr.201337936
2014
Crystal Structure and Self-Interaction of the Type VI Secretion Tail-Tube Protein from Enteroaggregative Escherichia coli
Badreddine Douzi, Silvia Spinelli, Stéphanie Blangy, Alain Roussel, Eric Durand, Yannick R Brunet, E. Cascales, Christian Cambillau
PLoS ONE 9:e86918 (2014)10.1371/journal.pone.0086918
2014
VgrG, Tae, Tle, and beyond: the versatile arsenal of Type VI secretion effectors.
Eric Durand, Christian Cambillau, E. Cascales, Laure Journet
Trends Microbiol. 22:498-507 (2014)10.1016/j.tim.2014.06.004
2014
VgrG, Tae, Tle, and beyond: the versatile arsenal of Type VI secretion effectors
Eric Durand, Christian Cambillau, E. Cascales, Laure Journet
Trends in Microbiology 22:498-507 (2014)10.1016/j.tim.2014.06.004
2014
IHF is required for the transcriptional regulation of the Desulfovibrio vulgaris Hildenborough orp operons
Anouchka Fiévet, E. Cascales, Odile Valette, Alain Dolla, Corinne Aubert
PLoS ONE 9:e86507 (2014)10.1371/journal.pone.0086507
2014
Transcriptional Frameshifting Rescues Citrobacter rodentium Type VI Secretion by the Production of Two Length Variants from the Prematurely Interrupted tssM Gene
Erwan Gueguen, Norma M. Wills, John F. Atkins, E. Cascales
PLoS Genetics 10 (2014)10.1371/journal.pgen.1004869
2014
Architecture and assembly of the Type VI secretion system
Abdelrahim Zoued, Yannick Brunet, Eric Durand, Marie-Stéphanie Aschtgen, Laureen Logger, Badreddine Douzi, Laure Journet, Christian Cambillau, E. Cascales
Biochimica et Biophysica Acta - Molecular Cell Research 1843:1664-1673 (2014)10.1016/j.bbamcr.2014.03.018
2014
Architecture and assembly of the Type VI secretion system.
Abdelrahim Zoued, Yannick R Brunet, Eric Durand, Marie-Stéphanie Aschtgen, Laureen Logger, Badreddine Douzi, Laure Journet, Christian Cambillau, E. Cascales
Biochim. Biophys. Acta 1843:1664-73 (2014)10.1016/j.bbamcr.2014.03.018
2013
Imaging type VI secretion-mediated bacterial killing.
Yannick R Brunet, Leon Espinosa, Seddik Harchouni, Tam Mignot, E. Cascales
Cell Rep 3:36-41 (2013)10.1016/j.celrep.2012.11.027
2013
Imaging Type VI Secretion-Mediated Bacterial Killing
Yannick r. Brunet, Leon Espinosa, Seddik Harchouni, Tâm Mignot, E. Cascales
Cell Reports 3:36-41 (2013)10.1016/j.celrep.2012.11.027
2013
DNA Substrate-Induced Activation of the Agrobacterium VirB/VirD4 Type IV Secretion System
E. Cascales, Krishnamohan Atmakuri, Mayukh Sarkar, Peter Christie
Journal of Bacteriology 195:2691-2704 (2013)10.1128/JB.00114-13
2013
DNA substrate-induced activation of the Agrobacterium VirB/VirD4 type IV secretion system.
E. Cascales, Krishnamohan Atmakuri, Mayukh K Sarkar, Peter J Christie
Journal of Bacteriology 195:2691-704 (2013)10.1128/JB.00114-13
2013
Expression of a Yersinia pseudotuberculosis Type VI Secretion System Is Responsive to Envelope Stresses through the OmpR Transcriptional Activator
Erwan Gueguen, Eric Durand, Xiang Zhang, Quentin D’amalric, Laure Journet, E. Cascales
PLoS ONE 8:e66615 (2013)10.1371/journal.pone.0066615
2013
Promoter swapping unveils the role of the Citrobacter rodentium CTS1 type VI secretion system in interbacterial competition.
Erwan Gueguen, E. Cascales
Applied and Environmental Microbiology 79:32-8 (2013)10.1128/AEM.02504-12
2013
Transcription of the Escherichia coli fatty acid synthesis operon fabHDG is directly activated by FadR and inhibited by ppGpp.
Laetitia My, Brian Rekoske, Justin J Lemke, Julie Viala, Richard L Gourse, Emmanuelle Bouveret
Journal of Bacteriology 195:3784-95 (2013)10.1128/JB.00384-13
2013
Posttranslational maturation of the invasion acyl carrier protein of Salmonella enterica serovar Typhimurium requires an essential phosphopantetheinyl transferase of the fatty acid biosynthesis pathway.
Julie Viala, Rémy Puppo, Lætitia My, Emmanuelle Bouveret
Journal of Bacteriology 195:4399-405 (2013)10.1128/JB.00472-13
2013
TssK Is a Trimeric Cytoplasmic Protein Interacting with Components of Both Phage-like and Membrane Anchoring Complexes of the Type VI Secretion System
Abdelrahim Zoued, Eric Durand, Cecilia C. Bebeacua, Yannick R Brunet, Badreddine Douzi, Christian Cambillau, E. Cascales, Laure Journet
Journal of Biological Chemistry 288:27031-27041 (2013)10.1074/jbc.M113.499772
Collaborations
- National
- Sophie Abby and Nelle Varoquaux, TIMC UMR5525, Grenoble
- Nicolas Barnich, M2iSH UMR1071, Clermont-Ferrand
- Stéphane Canaan, LISM UMR7255, Marseille
- Julia Chamot-Rooke, Pasteur Institute USR2000, Paris
- Rémi Fronzes, MFP UMR5234, Pessac
- Jean-Marc Ghigo, Pasteur Institute UMR2001, Paris
- Alain Givaudan and Julien Brillard, DGIMI, INRAE, Montpellier
- Marianne Ilbert, BIP UMR7281, Marseille
- Olivier Lambert, CBNM, Pessac
- Annabelle Merieau, LMSM UR4312, Evreux
- Sylvie Miquel and Christiane Forestier, LMGE UMR6023, Clermont-Ferrand
- Olivier Namy, I2BC UMR9198, Gif-sur-Yvette
- Alain Roussel and Philippe Leone, LISM UMR7255, Marseille
- International
- Carlos Blondel, Universidad Andres Bello, Santiago, Chile
- Bo Hu, McGovern Medical School, The University of Texas, USA
- Susann Müller, Helmholtz Centre for Environmental Research GmbH – UFZ, Leipzig, Germany
- Eric Reynolds and Paul Veith, The University of Melbourne, Australia
- Patrick Viollier, Université de Genève, Suisse
- Gong Zhang, Institute of Life and Health Engineering, Jinan University, Guangzhou, China
Funding
