The main objective of our team is to provide information on the molecular mechanisms underlying the assembly, the structure and the mode of action of multiprotein complexes associated with the cell envelope in Gram-negative bacteria, with an emphasis on secretion systems.

We currently develop several lines of study on the type VI secretion system (T6SS), a contractile nanomachine that uses a spring-like mechanism to deliver effectors in target cells. We focus our efforts on understanding the regulation of the expression of T6SS gene clusters, on the structure of the apparatus, and defining how it works and how it propels effectors. An important line of study on the T6SS is to characterize effectors – their activities and targets – and how they are recruited, selected and mounted on the secretion system. A more recent project in our group wishes to define the architecture and the mechanism of action of the type IX secretion system (T9SS), a secretion system restricted to the Bacteroidetes phylum, and involved in pathogenesis and gliding motility. For all these projects, we used a combination of bacterial genetics, molecular biology, physiological tests, structural biology, and fluorescence microscopy.

1 – Architecture and biogenesis of the type VI secretion systems

The type VI secretion system (T6SS) is a multi-subunit machine that is widespread in Gram negative bacteria with an over-representation in Proteobacteria and Bacteroidetes. The T6SS is a contractile machine that uses a spring-like mechanism to propel an effector-loaded needle into the target. The T6SS comprises three sub-complexes: (i) the membrane complex that serves as docking station at the cell envelope and as channel for the passage of the needle during contraction, (ii) the baseplate, which is the assembly platform for (iii) the tail tube/sheath complex.

In the recent years we have focused our efforts on the understanding of the structure and assembly pathway of the membrane and baseplate subcomplexes. We have also identified subunits that coordinate or control tail tube/sheath assembly, termination or contraction. We collaborate with several colleagues in France or abroad, notably the group of R. Fronzes for electron microscopy structural studies.

2 – Activity and delivery of type VI secretion toxins

The type VI secretion system (T6SS) injects toxins to either prokaryotic or eukaryotic cells and is thus directly involved in virulence, and/or participate to clear and colonize niches by killing bacterial competitors. The competitive advantage provided by T6SS on intra and inter-species bacterial interaction in the gut and thus on the composition of the microbiota was recently highlighted. Our goal is to identify and determine the mode of action and the targets of T6SS effector toxins, using pathogenic Escherichia coli and related species as models. We also want to understand how T6SS effectors are loaded on the T6SS machine for delivery and how they are activated in the target cell. Specifically, in the recent years, we characterized an antibacterial phospholipase toxin of enteroaggregative E. coli and its immunity protein Tli1. Three Tle1 toxins interact directly with VgrG, the needle spike of the machine, for delivery in the periplasm of target cells.

3 – The Type IX secretion in pathogenesis and gliding motility

The phylum Bacteroidetes is under-studied despite many of its members being important pathogens and commensals. These bacteria distinguish themselves by using the type IX secretion system (T9SS). This machinery is composed of at least 18 proteins assembled to secrete effectors supporting a wide variety of functions.

Notably, in Porphyromonas gingivalis, the main microorganism responsible for periodontitis, the T9SS secretes a cocktail of adhesins and proteases called gingipains to promote tissue invasion.

Several Bacteroidetes members also use the T9SS for gliding. This motility process relies on adhesins translocated to the cell surface and set into motion by the T9SS. Using a multidisciplinary approach, we are dedicated to understand the T9SS at multiple scales, from the organization of the machinery, the secretion pathway, the biological functions, to the link with gliding. We collaborate with Alain Roussel at AFMB and have recently biochemically and structurally characterized key subunits and subcomplexes.

Publications

2021
The Azospirillum brasilense Type VI secretion system promotes cell aggregation, biocontrol protection against phytopathogens and attachment to the microalgae Chlorella sorokiniana

Fabricio Cassan, Anahí Coniglio, Edgar Amavizca, Guillermo Maroniche, E. Cascales, Yoav Bashan, Luz De‐bashan

Environmental Microbiology (2021)10.1111/1462-2920.15749

2021
Dual Regulation of Phosphatidylserine Decarboxylase Expression by Envelope Stress Responses

Yasmine Hassoun, Julia Bartoli, Astrid Wahl, Julie Viala, Emmanuelle Bouveret

Frontiers in Molecular Biosciences 8 (2021)10.3389/fmolb.2021.665977

2021
Activity, delivery and diversity of Type VI secretion effectors

Dukas Jurėnas, Laure Journet

Molecular Microbiology 115:383-394 (2021)10.1111/mmi.14648

2021
Photorhabdus antibacterial Rhs polymorphic toxin inhibits translation through ADP-ribosylation of 23S ribosomal RNA

Dukas Jurėnas, Amaury Payelleville, Mohammad Roghanian, Kathryn Turnbull, Alain Givaudan, Julien Brillard, Vasili Hauryliuk, E. Cascales

Nucleic Acids Research 49:8384-8395 (2021)10.1093/nar/gkab608

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2021
FisB relies on homo-oligomerization and lipid binding to catalyze membrane fission in bacteria

Ane Landajuela, Martha Braun, Christopher Rodrigues, Alejandro Martínez-Calvo, Thierry Doan, Florian Horenkamp, Anna Andronicos, Vladimir Shteyn, Nathan Williams, Chenxiang Lin, Ned Wingreen, David Rudner, Erdem Karatekin

PLoS Biology 19:e3001314 (2021)10.1371/journal.pbio.3001314

2021
Molecular Strategies Underlying Porphyromonas gingivalis Virulence

Ignacio Lunar Silva, E. Cascales

Journal of Molecular Biology 433:166836 (2021)10.1016/j.jmb.2021.166836

2021
Anchoring the T6SS to the cell wall: Crystal structure of the peptidoglycan binding domain of the TagL accessory protein

van Son Nguyen, Silvia Spinelli, E. Cascales, Alain Roussel, Christian Cambillau, Philippe Leone

PLoS ONE 16:e0254232 (2021)10.1371/journal.pone.0254232

2020
2020
SlyA Transcriptional Regulator Is Not Directly Affected by ppGpp Levels

Julia Bartoli, Julie Viala, Emmanuelle Bouveret

Frontiers in Microbiology 11:1856 (2020)10.3389/fmicb.2020.01856

2020
Structural basis for loading and inhibition of a bacterial T6 SS phospholipase effector by the VgrG spike

Nicolas Flaugnatti, Chiara Rapisarda, Martial Rey, Solène Beauvois, Viet Anh Nguyen, Stéphane Canaan, Eric Durand, Julia Chamot‐rooke, E. Cascales, Rémi Fronzes, Laure Journet

EMBO Journal 39:e104129 (2020)10.15252/embj.2019104129

2019
Deciphering the specific interaction between the acyl carrier protein IacP and the T3SS‐major hydrophobic translocator SipB from Salmonella

Mickaël Canestrari, Bastien Serrano, Julia Bartoli, Valérie Prima, Olivier Bornet, Rémy Puppo, Emmanuelle Bouveret, Françoise Guerlesquin, Julie Viala

FEBS Letters 594:251-265 (2019)10.1002/1873-3468.13593

2019
Structure and Activity of the Type VI Secretion System

Yassine Cherrak, Nicolas Flaugnatti, Eric Durand, Laure Journet, E. Cascales

Microbiology Spectrum 7 (2019)10.1128/microbiolspec.PSIB-0031-2019

2019
In situ and high-resolution Cryo-EM structure of the Type VI secretion membrane complex

Chiara Rapisarda, Yassine Cherrak, Romain Kooger, Victoria Schmidt, Riccardo Pellarin, Laureen Logger, E. Cascales, Martin Pilhofer, Eric Durand, Rémi Fronzes

EMBO Journal 38 (2019)10.15252/embj.2018100886

2019
Cell width dictates Type VI secretion tail length

Yoann Santin, Thierry Doan, Laure Journet, E. Cascales

Current Biology - CB (2019)10.1016/j.cub.2019.08.058

2019
Role and recruitment of the TagL peptidoglycan-binding protein during Type VI secretion system biogenesis

Yoann Santin, Claire Camy, Abdelrahim Zoued, Thierry Doan, Marie-Stéphanie Aschtgen, E. Cascales

Journal of Bacteriology (2019)10.1128/JB.00173-19

2018
Antibacterial Weapons: Targeted Destruction in the Microbiota

Benoit Chassaing, E. Cascales

Trends in Microbiology 26:329-338 (2018)10.1016/j.tim.2018.01.006

2018
Biogenesis and structure of a type VI secretion baseplate

Yassine Cherrak, Chiara Rapisarda, Riccardo Pellarin, Guillaume Bouvier, Benjamin Bardiaux, Fabrice Allain, Christian Malosse, Martial Rey, Julia Chamot-Rooke, E. Cascales, Rémi Fronzes, Eric Durand

Nature Microbiology 3:1404-1416 (2018)10.1038/s41564-018-0260-1

2018
Structure–Function Analysis of the C-Terminal Domain of the Type VI Secretion TssB Tail Sheath Subunit

Badreddine Douzi, Laureen Logger, Silvia Spinelli, Stephanie Blangy, Christian Cambillau, E. Cascales

Journal of Molecular Biology 430:297-309 (2018)10.1016/j.jmb.2017.11.015

2018
Type IX secretion system PorM and gliding machinery GldM form extended arches spanning the periplasmic space

Philippe Leone, Jennifer Roche, Maxence Vincent, Quang Hieu Tran, Aline Desmyter, E. Cascales, Christine Kellenberger, Christian Cambillau, Alain Roussel

Nature Communications 9 (2018)10.1038/s41467-017-02784-7

2018
Towards a complete structural deciphering of Type VI secretion system

van Son Nguyen, Badreddine Douzi, Eric Durand, Alain Roussel, E. Cascales, Christian Cambillau

Current Opinion in Structural Biology 49:77-84 (2018)10.1016/j.sbi.2018.01.007

2018
The gp27-like Hub of VgrG Serves as Adaptor to Promote Hcp Tube Assembly

Melvin Renault, Jordi Zamarreño Beas, Badreddine Douzi, Maïalène Chabalier, Abdelrahim Zoued, Yannick Brunet, Christian Cambillau, Laure Journet, E. Cascales

Journal of Molecular Biology 430:3143-3156 (2018)10.1016/j.jmb.2018.07.018

2018
In vivo TssA proximity labeling reveals temporal interactions during Type VI secretion 1 biogenesis and TagA, a protein that stops and holds the sheath.

Yoann Santin, Thierry Doan, Régine Lebrun, Leon Espinosa, Laure Journet, E. Cascales

Nature Microbiology 3:1304-1313 (2018)10.1038/s41564-018-0234-3

2018
Tryptophan-mediated Dimerization of the TssL Transmembrane Anchor Is Required for Type VI Secretion System Activity

Abdelrahim Zoued, Jean-Pierre Duneau, Eric Durand, Alexandre España, Laure Journet, Françoise Guerlesquin, E. Cascales

Journal of Molecular Biology 430:987-1003 (2018)10.1016/j.jmb.2018.02.008

2017
Microbiology: And Amoebophilus Invented the Machine Gun!

E. Cascales

Current Biology - CB 27:R1170-R1173 (2017)10.1016/j.cub.2017.09.025

2017
Type VI secretion TssK baseplate protein exhibits structural similarity with phage receptor-binding proteins and evolved to bind the membrane complex

van Son Nguyen, Laureen Logger, Silvia Spinelli, Pierre Legrand, Thi Thanh Huyen Pham, Thi Trang Nhung Trinh, Yassine Cherrak, Abdelrahim Zoued, Aline Desmyter, Eric Durand, Alain Roussel, Christine Kellenberger, E. Cascales, Christian Cambillau

Nature Microbiology 2:17103 (2017)10.1038/nmicrobiol.2017.103

2017
Acylation of the Type 3 Secretion System Translocon Using a Dedicated Acyl Carrier Protein

Julie Viala, Valerie Prima, Remy Puppo, Rym Agrebi, Mickael J. Canestrari, Sabrina Lignon, Nicolas Chauvin, Stephane Meresse, Tam Mignot, Regine Lebrun, Emmanuelle Bouveret

PLoS Genetics 13 (2017)10.1371/journal.pgen.1006556

2017
Characterization of the Porphyromonas gingivalis Type IX Secretion Trans-envelope PorKLMNP Core Complex

Maxence Vincent, Mickaël Canestrari, Philippe Leone, Julien Stathopulos, Bérengère Ize, Abdelrahim Zoued, Christian Cambillau, Christine Kellenberger, Alain Roussel, E. Cascales

Journal of Biological Chemistry 292:3252-3261 (2017)10.1074/jbc.M116.765081

2017
TssA: The cap protein of the Type VI secretion system tail

Abdelrahim Zoued, Eric Durand, Yoann Santin, Laure Journet, Alain Roussel, Christian Cambillau, E. Cascales

BioEssays 39 (2017)10.1002/bies.201600262

2016
Meeting report: Adaptation and communication of bacterial pathogens

Laurent Aussel, Carmen Beuzón, E. Cascales

Virulence 7:481-490 (2016)10.1080/21505594.2016.1152441

2016
Polyamino-Isoprenic Derivatives Block Intrinsic Resistance of P. aeruginosa to Doxycycline and Chloramphenicol In Vitro

Diane Borselli, Aurélie Lieutaud, Hélène Thefenne, Eric Garnotel, Jean-Marie Pagès, Jean Michel Brunel, Jean-Michel Bolla, E. Cascales

PLoS ONE 11:e154490 (2016)10.1371/journal.pone.0154490

2016
Structure and specificity of the Type VI secretion system ClpV-TssC interaction in enteroaggregative Escherichia coli

Badreddine Douzi, Yannick Brunet, Silvia Spinelli, Valentine Lensi, Stephanie Blangy, Anant Kumar, Laure Journet, Pierre Legrand, E. Cascales, Christian Cambillau

Scientific Reports 6 (2016)10.1038/srep34405

2016
A phospholipase A 1 antibacterial Type VI secretion effector interacts directly with the C-terminal domain of the VgrG spike protein for delivery

Nicolas Flaugnatti, Thi Thu Hang Le, Stéphane Canaan, Marie-Stéphanie Aschtgen, Vân Nguyên, Stephanie Blangy, Christine Kellenberger, Alain Roussel, Christian Cambillau, E. Cascales, Laure Journet

Molecular Microbiology 99:1099-1118 (2016)10.1111/mmi.13292

2016
Molecular Dissection of the Interface between the Type VI Secretion TssM Cytoplasmic Domain and the TssG Baseplate Component

Laureen Logger, Marie-Stéphanie Aschtgen, Marie Guérin, E. Cascales, Eric Durand

Journal of Molecular Biology 428:4424-4437 (2016)10.1016/j.jmb.2016.08.032

2016
Coexpression of Escherichia coli obgE, Encoding the Evolutionarily Conserved Obg GTPase, with Ribosomal Proteins L21 and L27.

Rim Maouche, Hector L Burgos, Laetitia My, Julie Viala, Richard L Gourse, Emmanuelle Bouveret

Journal of Bacteriology 198:1857-67 (2016)10.1128/JB.00159-16

2016
Salmonella Typhimurium utilizes a T6SS-mediated antibacterial weapon to establish in the host gut

Thibault Sana, Nicolas Flaugnatti, Kyler Lugo, Lilian Lam, Amanda Jacobson, Virginie Baylot, Eric Durand, Laure Journet, E. Cascales, Denise Monack

Proceedings of the National Academy of Sciences of the United States of America 113:E5044-E5051 (2016)10.1073/pnas.1608858113

2016
The iron-sulfur cluster sensor IscR is a negative regulator of Spi1 Type III Secretion System in Salmonella enterica Cellular Microbiology

Alexandra Vergnes, Julie Viala, Ouadah‐tsabet Rabah, Bérengère Pocachard, Laurent Loiseau, Stephane Meresse, Frédéric Barras, Laurent Aussel

Cellular Microbiology 19:e12680 (2016)10.1111/cmi.12680

2016
Priming and polymerization of a bacterial contractile tail structure. 1" 2" 17

Abdelrahim Zoued, Eric Durand, Yannick Brunet, Silvia Spinelli, Badreddine Douzi, Mathilde Guzzo, Nicolas Flaugnatti, Pierre Legrand, Laure Journet, Rémi Fronzes, Tam Mignot, Christian Cambillau, E. Cascales

Nature 531:59-63 (2016)10.1038/nature17182

2016
Structure–Function Analysis of the TssL Cytoplasmic Domain Reveals a New Interaction between the Type VI Secretion Baseplate and Membrane Complexes

Abdelrahim Zoued, Chloé Cassaro, Eric Durand, Badreddine Douzi, Alexandre España, Christian Cambillau, Laure Journet, E. Cascales

Journal of Molecular Biology 428:4413-4423 (2016)10.1016/j.jmb.2016.08.030

2015
H-NS Silencing of the Salmonella Pathogenicity Island 6-Encoded Type VI Secretion System Limits Salmonella enterica Serovar Typhimurium Interbacterial Killing

Yannick R. Brunet, Ahmad Khodr, Laureen Logger, Laurent Aussel, Tam Mignot, Sylvie Rimsky, E. Cascales

Infection and Immunity 83:2738-2750 (2015)10.1128/IAI.00198-15

2015
Biogenesis and structure of a Type VI secretion membrane core complex

Eric Durand, van Son Nguyen, Abdelrahim Zoued, Laureen Logger, Gerard Pehau-Arnaudet, Marie-Stéphanie Aschtgen, Silvia Spinelli, Aline Desmyter, Benjamin Bardiaux, Annick Dujeancourt, Alain Roussel, Christian Cambillau, E. Cascales, Rémi Fronzes

Nature 523:555-560 (2015)10.1038/nature14667

2015
Inhibition of Type VI Secretion by an Anti-TssM Llama Nanobody

van Son Nguyen, Laureen Logger, Silvia Spinelli, Aline Desmyter, Thi Thu Hang Le, Christine Kellenberger, Badreddine Douzi, Eric Durand, Alain Roussel, E. Cascales, Christian Cambillau

PLoS ONE 10:1-14 (2015)

2015
Production, crystallization and X-ray diffraction analysis of a complex between a fragment of the TssM T6SS protein and a camelid nanobody

van Son Nguyen, Silvia Spinelli, Aline Desmyter, Thi Thu Hang Le, Christine Kellenberger, E. Cascales, Christian Cambillau, Alain Roussel

Acta crystallographica. Section F, Structural biology communications 71:266-271 (2015)10.1107/S2053230X15000709

2015
Crystallization and preliminary X-ray analysis of the C-terminal fragment of PorM, a subunit of the Porphyromonas gingivalis type IX secretion system

Julien Stathopulos, Christian Cambillau, E. Cascales, Alain Roussel, Philippe Leone

Acta crystallographica. Section F, Structural biology communications 71:71-74 (2015)10.1107/S2053230X1402559X

2014
Anchoring the type VI secretion system to the peptidoglycan: TssL, TagL, TagP... what else?

Marie-Stéphanie Aschtgen, Mark Thomas, E. Cascales

Virulence 1:535-540 (2014)10.4161/viru.1.6.13732

2014
Type VI secretion and bacteriophage tail tubes share a common assembly pathway.

Yannick R Brunet, Jérôme Hénin, Hervé Celia, E. Cascales

EMBO Reports 15:315-21 (2014)10.1002/embr.201337936

2014
Type VI secretion and bacteriophage tail tubes share a common assembly pathway

Yannick Brunet, Jérôme Hénin, Hervé Celia, E. Cascales

EMBO Reports 15:315-321 (2014)10.1002/embr.201337936

2014
Crystal Structure and Self-Interaction of the Type VI Secretion Tail-Tube Protein from Enteroaggregative Escherichia coli

Badreddine Douzi, Silvia Spinelli, Stéphanie Blangy, Alain Roussel, Eric Durand, Yannick Brunet, E. Cascales, Christian Cambillau

PLoS ONE 9:e86918 (2014)10.1371/journal.pone.0086918

2014
VgrG, Tae, Tle, and beyond: the versatile arsenal of Type VI secretion effectors

Eric Durand, Christian Cambillau, E. Cascales, Laure Journet

Trends in Microbiology 22:498-507 (2014)10.1016/j.tim.2014.06.004

2014
VgrG, Tae, Tle, and beyond: the versatile arsenal of Type VI secretion effectors.

Eric Durand, Christian Cambillau, E. Cascales, Laure Journet

Trends Microbiol. 22:498-507 (2014)10.1016/j.tim.2014.06.004

2014
IHF Is Required for the Transcriptional Regulation of the Desulfovibrio vulgaris Hildenborough orp Operons

Anouchka Fiévet, E. Cascales, Odile Valette, Alain Dolla, Corinne Aubert

PLoS ONE 9:e86507 (2014)10.1371/journal.pone.0086507

2014
IHF is required for the transcriptional regulation of the Desulfovibrio vulgaris Hildenborough orp operons

Anouchka Fiévet, E. Cascales, Odile Valette, Alain Dolla, Corinne Aubert

PLoS ONE 9:e86507 (2014)10.1371/journal.pone.0086507

2014
Architecture and assembly of the Type VI secretion system

Abdelrahim Zoued, Yannick Brunet, Eric Durand, Marie-Stéphanie Aschtgen, Laureen Logger, Badreddine Douzi, Laure Journet, Christian Cambillau, E. Cascales

Biochimica et Biophysica Acta - Molecular Cell Research 1843:1664-1673 (2014)10.1016/j.bbamcr.2014.03.018

2014
Architecture and assembly of the Type VI secretion system.

Abdelrahim Zoued, Yannick R Brunet, Eric Durand, Marie-Stéphanie Aschtgen, Laureen Logger, Badreddine Douzi, Laure Journet, Christian Cambillau, E. Cascales

Biochim. Biophys. Acta 1843:1664-73 (2014)10.1016/j.bbamcr.2014.03.018

2013
Imaging type VI secretion-mediated bacterial killing.

Yannick R Brunet, Leon Espinosa, Seddik Harchouni, Tam Mignot, E. Cascales

Cell Rep 3:36-41 (2013)10.1016/j.celrep.2012.11.027

2013
Imaging Type VI Secretion-Mediated Bacterial Killing

Yannick r. Brunet, Leon Espinosa, Seddik Harchouni, Tâm Mignot, E. Cascales

Cell Reports 3:36-41 (2013)10.1016/j.celrep.2012.11.027

2013
DNA Substrate-Induced Activation of the Agrobacterium VirB/VirD4 Type IV Secretion System

E. Cascales, Krishnamohan Atmakuri, Mayukh Sarkar, Peter Christie

Journal of Bacteriology 195:2691-2704 (2013)10.1128/JB.00114-13

2013
DNA substrate-induced activation of the Agrobacterium VirB/VirD4 type IV secretion system.

E. Cascales, Krishnamohan Atmakuri, Mayukh K Sarkar, Peter J Christie

Journal of Bacteriology 195:2691-704 (2013)10.1128/JB.00114-13

2013
2013
Transcription of the Escherichia coli fatty acid synthesis operon fabHDG is directly activated by FadR and inhibited by ppGpp.

Laetitia My, Brian Rekoske, Justin J Lemke, Julie Viala, Richard L Gourse, Emmanuelle Bouveret

Journal of Bacteriology 195:3784-95 (2013)10.1128/JB.00384-13

2013
Dissection of the TssB-TssC Interface during Type VI Secretion Sheath Complex Formation

Xiang Zhang, Yannick Brunet, Laureen Logger, Badreddine Douzi, Christian Cambillau, Laure Journet, E. Cascales

PLoS ONE 8:e81074 (2013)10.1371/journal.pone.0081074

2013
TssK Is a Trimeric Cytoplasmic Protein Interacting with Components of Both Phage-like and Membrane Anchoring Complexes of the Type VI Secretion System

Abdelrahim Zoued, Eric Durand, Cecilia Bebeacua, Yannick Brunet, Badreddine Douzi, Christian Cambillau, E. Cascales, Laure Journet

Journal of Biological Chemistry 288:27031-27041 (2013)10.1074/jbc.M113.499772

2012
The C‐tail anchored TssL subunit, an essential protein of the enteroaggregative Escherichia coli Sci‐1 Type VI secretion system, is inserted by YidC

Marie-Stéphanie Aschtgen, Abdelrahim Zoued, Roland Lloubès, Laure Journet, E. Cascales

MicrobiologyOpen 1:71-82 (2012)10.1002/mbo3.9

2012
Structural biology of type VI secretion systems

E. Cascales, Christian Cambillau

Philosophical Transactions of the Royal Society B: Biological Sciences 367:1102-1111 (2012)10.1098/rstb.2011.0209

2012
Structural biology of type VI secretion systems.

E. Cascales, Christian Cambillau

Philos. Trans. R. Soc. Lond., B, Biol. Sci. 367:1102-11 (2012)10.1098/rstb.2011.0209

2012
Crystal Structure of the VgrG1 Actin Cross-linking Domain of the Vibrio cholerae Type VI Secretion System

Eric Durand, Estelle Derrez, Gilles Audoly, Silvia Spinelli, Miguel Ortiz-Lombardia, Didier Raoult, E. Cascales, Christian Cambillau

Journal of Biological Chemistry 287:38190-38199 (2012)10.1074/jbc.M112.390153

2012
Crystal structure of the VgrG1 actin cross-linking domain of the Vibrio cholerae type VI secretion system.

Eric Durand, Estelle Derrez, Gilles Audoly, Silvia Spinelli, Miguel Ortiz-Lombardia, Didier Raoult, E. Cascales, Christian Cambillau

Journal of Biological Chemistry 287:38190-9 (2012)10.1074/jbc.M112.390153

2012
Structural characterization and oligomerization of the TssL protein, a component shared by bacterial type VI and type IVb secretion systems.

Eric Durand, Abdelrahim Zoued, Silvia Spinelli, Paul J H Watson, Marie-Stéphanie Aschtgen, Laure Journet, Christian Cambillau, E. Cascales

Journal of Biological Chemistry 287:14157-68 (2012)10.1074/jbc.M111.338731

2012
Strategies to adapt cellular processes to nutrient availability in bacteria.

Elodie Foulquier, Thierry Doan, Frederique Pompeo, Anne Galinier

Frontiers in Bioscience (Scholar edition) 4:1007-16 (2012)

2012
Energetics of colicin import revealed by genetic cross-complementation between the Tol and Ton systems.

Roland Lloubes, Emilie Goemaere, Xiang Zhang, E. Cascales, Denis Duché

Biochem. Soc. Trans. 40:1480-5 (2012)10.1042/BST20120181

2012
Energetics of colicin import revealed by genetic cross-complementation between the Tol and Ton systems

Roland Lloubès, Emilie Goemaere, Xiang Zhang, E. Cascales, Denis Duché

Biochemical Society Transactions 40:1480-1485 (2012)10.1042/BST20120181

2012
Structure and regulation of the type VI secretion system.

Julie M Silverman, Yannick R Brunet, E. Cascales, Joseph D Mougous

Annual Review of Microbiology 66:453-72 (2012)10.1146/annurev-micro-121809-151619

2012
Structure and Regulation of the Type VI Secretion System

Julie Silverman, Yannick Brunet, E. Cascales, Joseph Mougous

Annual Review of Microbiology 66:453-472 (2012)10.1146/annurev-micro-121809-151619

2012
The C-tail anchored TssL subunit, an essential protein of the enteroaggregative Escherichia coli Sci-1 Type VI secretion system, is inserted by YidC.

Abdelrahim Zoued, Roland Lloubes, Laure Journet, E. Cascales, Marie-Stéphanie Aschtgen

MicrobiologyOpen 1:71-82 (2012)10.1002/mbo3.9

2011
An Agrobacterium VirB10 mutation conferring a type IV secretion system gating defect.

Lois M Banta, Jennifer E Kerr, E. Cascales, Meghan E Giuliano, Megan E Bailey, Cedar Mckay, Vidya Chandran, Gabriel Waksman, Peter J Christie

Journal of Bacteriology 193:2566-74 (2011)10.1128/JB.00038-11

2011
Regulation of type VI secretion gene clusters by sigma54 and cognate enhancer binding proteins.

Christophe S Bernard, Yannick R Brunet, Marthe Gavioli, Roland Lloubes, E. Cascales

Journal of Bacteriology 193:2158-67 (2011)10.1128/JB.00029-11

2011
An Epigenetic Switch Involving Overlapping Fur and DNA Methylation Optimizes Expression of a Type VI Secretion Gene Cluster

Yannick Brunet, Christophe Bernard, Marthe Gavioli, Roland Lloubès, E. Cascales

PLoS Genetics 7:e1002205 (2011)10.1371/journal.pgen.1002205

2011
Towards a Structural Comprehension of Bacterial Type VI Secretion Systems: Characterization of the TssJ-TssM Complex of an Escherichia coli Pathovar

Catarina Felisberto-Rodrigues, Eric Durand, Marie-Stéphanie Aschtgen, Stéphanie Blangy, Miguel Ortiz-Lombardia, Badreddine Douzi, Christian Cambillau, E. Cascales

PLoS Pathogens 7:e1002386 (2011)10.1371/journal.ppat.1002386

2011
The anaerobe-specific orange protein complex of Desulfovibrio vulgaris hildenborough is encoded by two divergent operons coregulated by ?54 and a cognate transcriptional regulator.

Anouchka Fiévet, Laetitia My, E. Cascales, Mireille Ansaldi, Sofia R Pauleta, Isabel Moura, Zorah Dermoun, Christophe S Bernard, Alain Dolla, Corinne Aubert

Journal of Bacteriology 193:3207-19 (2011)10.1128/JB.00044-11

2011
Motor-driven intracellular transport powers bacterial gliding motility.

Mingzhai Sun, Morgane Wartel, E. Cascales, Joshua W Shaevitz, Tam Mignot

Proceedings of the National Academy of Sciences of the United States of America 108:7559-64 (2011)10.1073/pnas.1101101108

2011
Mapping the interactions between Escherichia coli TolQ transmembrane segments.

Xiang Y-Z Zhang, Emilie L Goemaere, Nadir Seddiki, Hervé Celia, Marthe Gavioli, E. Cascales, Roland Lloubes

Journal of Biological Chemistry 286:11756-64 (2011)10.1074/jbc.M110.192773

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